INV5_ORYSJ
ID INV5_ORYSJ Reviewed; 542 AA.
AC Q56UD1; Q7XPK5;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 3.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 5;
DE EC=3.2.1.26;
DE AltName: Full=Cell wall beta-fructosidase 5;
DE AltName: Full=Invertase 5;
DE AltName: Full=OsCIN5;
DE AltName: Full=Sucrose hydrolase 5;
GN Name=CIN5; OrderedLocusNames=Os04g0664900, LOC_Os04g56930;
GN ORFNames=OSJNBa0087O24.4;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 17-542, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Nipponbare;
RX PubMed=15759120; DOI=10.1007/s00299-004-0910-z;
RA Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H.,
RA Lee K.-W., An G., Hahn T.-R., Jeon J.-S.;
RT "Molecular cloning and expression analysis of the cell-wall invertase gene
RT family in rice (Oryza sativa L.).";
RL Plant Cell Rep. 24:225-236(2005).
CC -!- FUNCTION: May play a role in stress response.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC the cell wall. {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and leaves.
CC {ECO:0000269|PubMed:15759120}.
CC -!- INDUCTION: By rice blast fungus (M.grisea) 48 hours after infection.
CC Down-regulated by sucrose in excised leaves.
CC {ECO:0000269|PubMed:15759120}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT84405.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAE03581.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL606646; CAE03581.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014960; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY578162; AAT84405.1; ALT_INIT; mRNA.
DR AlphaFoldDB; Q56UD1; -.
DR SMR; Q56UD1; -.
DR STRING; 4530.OS04T0664900-01; -.
DR PaxDb; Q56UD1; -.
DR PRIDE; Q56UD1; -.
DR eggNOG; KOG0228; Eukaryota.
DR InParanoid; Q56UD1; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.115.10.20; -; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF75005; SSF75005; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Cell wall; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW Reference proteome; Secreted.
FT CHAIN 1..542
FT /note="Beta-fructofuranosidase, insoluble isoenzyme 5"
FT /id="PRO_0000169867"
FT ACT_SITE 53
FT /evidence="ECO:0000250"
FT BINDING 50..53
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 69
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 77
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 114..115
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 178..179
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 233
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 510
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 516
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 389..436
FT /evidence="ECO:0000250"
SQ SEQUENCE 542 AA; 59533 MW; C5B64DB46B00FBDE CRC64;
MQLLVPIYKR HSNSNFKVSS CSSSPMCPVN GKLQLHDGRT AYHFQPAKFW QNDPNGPLYH
NGLYHFFYQY NPHGPLWDTG KLSWGHSVSG DLVNWAFLGT AIDPTDPFDV NGCWSGSATV
LLGGRPAFLY TGRDAGGVQV QNVSFAKNPL DPLLREWEKP SCNPIIAFPA DVINNNFRDP
TTAWLGRDGL WRMVVAAEVA GAGSALVYRS ADFLRWERNA APMHSSAAVP VLECPDFFPV
AEHGIDGLDT SANGGGTGVK HVLKLSEFDT HQDFYMVGRN RRVQWLWVNE YDSKADDVAK
GWAGVQAFPR KVWLDGDGKQ LLQWPVDEIE TLRTKRVGLQ GTEVKAGGLH EIVGVASSQA
DVEVVFEIPN LEDEAESFDP DWLDPHKLCK DKGAASAHGG VGPFGLIVMA SGDLQEQTAV
FFRVFKHHGK YKVFMCTDLT RSSTKADVYK DAYGGFVDVD IQKDKSISLR TLIDHSMIES
FGGGGRACIT TRVYPEHAAT SSSHLYVFNN GSGTVNVSKL EAWEMATATV NSADALDAIT
RS
