ID   INV6_ORYSJ              Reviewed;         596 AA.
AC   Q56UD0; Q0J9A1; Q7XPK6;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 6;
DE            EC=3.2.1.26;
DE   AltName: Full=Cell wall beta-fructosidase 6;
DE   AltName: Full=Invertase 6;
DE   AltName: Full=OsCIN6;
DE   AltName: Full=Sucrose hydrolase 6;
DE   Flags: Precursor;
GN   Name=CIN6; OrderedLocusNames=Os04g0664800, LOC_Os04g56920;
GN   ORFNames=OSJNBa0087O24.3;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15759120; DOI=10.1007/s00299-004-0910-z;
RA   Cho J.-I., Lee S.-K., Ko S., Kim H.-K., Jun S.-H., Lee Y.-H., Bhoo S.H.,
RA   Lee K.-W., An G., Hahn T.-R., Jeon J.-S.;
RT   "Molecular cloning and expression analysis of the cell-wall invertase gene
RT   family in rice (Oryza sativa L.).";
RL   Plant Cell Rep. 24:225-236(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC       the cell wall. {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots. Weakly expressed in flowers.
CC       {ECO:0000269|PubMed:15759120}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
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DR   EMBL; AY578163; AAT84406.1; -; mRNA.
DR   EMBL; AL606646; CAE03580.1; -; Genomic_DNA.
DR   EMBL; AP008210; BAF16086.2; -; Genomic_DNA.
DR   EMBL; AP014960; BAS91495.1; -; Genomic_DNA.
DR   RefSeq; XP_015634384.1; XM_015778898.1.
DR   AlphaFoldDB; Q56UD0; -.
DR   SMR; Q56UD0; -.
DR   STRING; 4530.OS04T0664800-00; -.
DR   CAZy; GH32; Glycoside Hydrolase Family 32.
DR   PaxDb; Q56UD0; -.
DR   PRIDE; Q56UD0; -.
DR   EnsemblPlants; Os04t0664800-00; Os04t0664800-00; Os04g0664800.
DR   GeneID; 4337312; -.
DR   Gramene; Os04t0664800-00; Os04t0664800-00; Os04g0664800.
DR   KEGG; osa:4337312; -.
DR   eggNOG; KOG0228; Eukaryota.
DR   HOGENOM; CLU_001528_6_0_1; -.
DR   InParanoid; Q56UD0; -.
DR   OMA; YGGPVCA; -.
DR   OrthoDB; 405663at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   Genevisible; Q56UD0; OS.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR001362; Glyco_hydro_32.
DR   InterPro; IPR013189; Glyco_hydro_32_C.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF08244; Glyco_hydro_32C; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SMART; SM00640; Glyco_32; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Cell wall; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..596
FT                   /note="Beta-fructofuranosidase, insoluble isoenzyme 6"
FT                   /id="PRO_0000033383"
FT   ACT_SITE        52
FT                   /evidence="ECO:0000250"
FT   BINDING         49..52
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         76
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         113..114
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         177..178
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         232
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        80
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        335
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        556
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        436..482
FT                   /evidence="ECO:0000250"
FT   CONFLICT        52..54
FT                   /note="Missing (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   596 AA;  64743 MW;  1FD62385B1389301 CRC64;
     MALAGLPLSV FAIAVHFCLV FSSSSSPPVC PANGHRDRTA YHFQPAKNWQ NDPNGPVYYN
     GMYHLFYQYN PHGALWDVGN LSWGHSVSGD LVNWAALDNA LDPTAPFDAN GCASGSVTIL
     PDGVPVVMYS GIDARRRQVQ NVAFPKNPRD PLLREWTKPG YNPVIPVPAD VSPDNFRDPT
     TAWLGSDGLW RFAISAVADG VGATLVYRSA DFLRWERNAA PLHASRDAVM AECPDLFPVA
     EHGEDGLDLD ASAIGGAGAG VRHVLKVSMP DTLEDYYMVG RYDDADDTFT VPPEDLEAHG
     DDYRRWRRID HGHLYASKTF YDAGKKRRVL WAWVNESDSE ADDVTKGWSG LQSFPRAVWL
     DEGGRQLVQW PVEEIETLRR KRGVLLGGNE VEAGGLREIG GIAGSQADVE VAFEIASLAG
     ADRLEPDHLR DPDALCGENG AAVHGGIGPF GLLVMASGDL RERTAVFFRV FRLSHGYTVL
     MCTDLTRSTS RAGVYKPSHG GFVDIDIEKD RAISLRTLID HSIVESFGGG GRTCMTARVY
     PEHVATGSSH LYVFNNASDA VKVSKLEAWE LATASVNAGD DGLISYGGPV CAAQVQ