ID   INV7_ORYSI              Reviewed;         596 AA.
AC   A2YZ01; Q56UC9; Q56UM5; Q6K311; Q9XGV7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 2.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Beta-fructofuranosidase, insoluble isoenzyme 7;
DE            EC=3.2.1.26;
DE   AltName: Full=Cell wall beta-fructosidase 7;
DE   AltName: Full=Invertase 7;
DE   AltName: Full=OsCIN7;
DE   AltName: Full=Sucrose hydrolase 7;
DE   Flags: Precursor;
GN   Name=CIN7; Synonyms=INV1; ORFNames=OsI_029544;
OS   Oryza sativa subsp. indica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39946;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. IR36;
RA   Fernandes S.Q., Dennis E.S., Dolferus R.;
RT   "Cloning of rice invertase genes.";
RL   Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. 93-11;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 503-596.
RC   STRAIN=cv. IR64;
RA   Ji X., Kathiresan A., Bennett J.;
RT   "Evolution of rice invertase gene family.";
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: May play a role in sucrose partitioning during seed
CC       development. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000305}. Secreted, cell wall {ECO:0000305}. Note=Associated to
CC       the cell wall. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD38399.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=AAV28806.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF155121; AAD38399.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; CM000134; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AY575555; AAV28806.1; ALT_FRAME; mRNA.
DR   AlphaFoldDB; A2YZ01; -.
DR   SMR; A2YZ01; -.
DR   STRING; 39946.A2YZ01; -.
DR   CAZy; GH32; Glycoside Hydrolase Family 32.
DR   Proteomes; UP000007015; Chromosome 9.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR001362; Glyco_hydro_32.
DR   InterPro; IPR013189; Glyco_hydro_32_C.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF08244; Glyco_hydro_32C; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SMART; SM00640; Glyco_32; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Cell wall; Disulfide bond; Glycoprotein; Glycosidase; Hydrolase;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..596
FT                   /note="Beta-fructofuranosidase, insoluble isoenzyme 7"
FT                   /id="PRO_0000303013"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000250"
FT   BINDING         51..54
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         70
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         78
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         115..116
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         179..180
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         234
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        82
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        330
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        552
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        432..478
FT                   /evidence="ECO:0000250"
FT   CONFLICT        19
FT                   /note="Missing (in Ref. 2; CM000134)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        22
FT                   /note="T -> I (in Ref. 1; AAD38399)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        309
FT                   /note="L -> V (in Ref. 1; AAD38399)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        318
FT                   /note="V -> A (in Ref. 1; AAD38399)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        381
FT                   /note="W -> R (in Ref. 1; AAD38399)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        388
FT                   /note="V -> A (in Ref. 1; AAD38399)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   596 AA;  65561 MW;  EBEA9B1D5BAF188B CRC64;
     MARLGLAVCA ASFHLFLLLA STSSLRRAPT EADTANHARR TAYHFQPAKN WQNDPNGPMY
     HNGMYHLFYQ YNPHSALWDI GNLSWGHSVS GDLLNWAALD TALDPTSPFD ANGCWSGSAT
     ILPGALPAIL YTGIDASKEQ VQNVAFAKNP SDPLLREWEK PAYNPVIALP ADVPGDKFRD
     PSTAWLGRDG LWRIAVSAEV DGVASTLVYR SKDFVRWERN AAPLHASRAA GMVECPDLFP
     VAERGEDGLD TSANGAGGVR HVLKLSVMDT LQDYYMVGTY DDAADAFSPA EPERGDDCRS
     WRRLDYGHLY ASKSFFDVRK NRRVLWAWAN ESDSQADDVA RGWSGVQTFP RKMWLAKDGK
     QLLQWPIEEI ETLRRKRAGL WRGTRLGVGA VQEIVGVASS QADVEVVFKI PSLEEAERVD
     DPNRLLDPQK LCGEKGAAVR GGVGPFGLLV MASGDLHEHT AVFFRVFRHH DKYKLLMCTD
     LTKSSTRAGV YKPAYGGFVD MDIDDHKTIS LRTLIDHSVV ESFGGGGRAC ITARVYPEHV
     ATSSSHLYVF NNGSDAVKVA KLEAWDLATA TVNVVVGDHH GLVAPALELE PTRTTQ