ID   INVA_MAIZE              Reviewed;         590 AA.
AC   P49174;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Beta-fructofuranosidase, cell wall isozyme;
DE            EC=3.2.1.26;
DE   AltName: Full=Invertase;
DE   AltName: Full=Sucrose hydrolase;
DE   Flags: Precursor;
OS   Zea mays (Maize).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX   NCBI_TaxID=4577;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Black Mexican Sweet;
RA   Shanker S.;
RL   Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10067};
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall.
CC   -!- INDUCTION: By wounding and bacterial infection.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family. {ECO:0000305}.
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DR   EMBL; U17695; AAA64487.1; -; mRNA.
DR   PIR; T02096; T02096.
DR   RefSeq; NP_001105369.1; NM_001111899.1.
DR   AlphaFoldDB; P49174; -.
DR   SMR; P49174; -.
DR   STRING; 4577.GRMZM2G139300_P01; -.
DR   CAZy; GH32; Glycoside Hydrolase Family 32.
DR   PaxDb; P49174; -.
DR   PRIDE; P49174; -.
DR   GeneID; 542314; -.
DR   KEGG; zma:542314; -.
DR   MaizeGDB; 113032; -.
DR   eggNOG; KOG0228; Eukaryota.
DR   OrthoDB; 405663at2759; -.
DR   Proteomes; UP000007305; Unplaced.
DR   ExpressionAtlas; P49174; baseline and differential.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR013320; ConA-like_dom_sf.
DR   InterPro; IPR001362; Glyco_hydro_32.
DR   InterPro; IPR018053; Glyco_hydro_32_AS.
DR   InterPro; IPR013189; Glyco_hydro_32_C.
DR   InterPro; IPR013148; Glyco_hydro_32_N.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF08244; Glyco_hydro_32C; 1.
DR   Pfam; PF00251; Glyco_hydro_32N; 1.
DR   SMART; SM00640; Glyco_32; 1.
DR   SUPFAM; SSF49899; SSF49899; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
DR   PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE   2: Evidence at transcript level;
KW   Cell wall; Glycoprotein; Glycosidase; Hydrolase; Reference proteome;
KW   Secreted; Signal.
FT   SIGNAL          1..28
FT                   /evidence="ECO:0000255"
FT   CHAIN           29..590
FT                   /note="Beta-fructofuranosidase, cell wall isozyme"
FT                   /id="PRO_0000033376"
FT   ACT_SITE        68
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10067"
FT   CARBOHYD        190
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        341
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   590 AA;  65198 MW;  296455E61E99B892 CRC64;
     MGTRPRGVVL APWAVVLVLV LALRLAGASH VIHRSLEAEA APSVPASIVS PLLRTGYHFQ
     PPMNWINDPN APLYYKGWYH LFYQYNPKGA VWGNIVWAHS VSRDLINWVA LEPAIYPSIP
     SDKYGCWSGS ATILEDGTPA ILYTGIDRAD INYQVQVLAL PKDASDPLLR EWEKPEEYNP
     VATPAAGGIN ATQFRDPTTA WRHAGHWRML VGSVRGARGM ALVYRSRDFR KWTKAKHPLH
     SAALTGMWEC PDFFPVSGPG LQAGLDTSAP GTKYVLKSSL DLTRYDYYTI GSYDGGKDRY
     YPDDPAGDYH HRRRYDYGNY YASKTFYDPV ERRRVLLGWA NESDSVPDDK AKGWAGIHAI
     PRKIWLDPTG KQLLQWPIHE VEKLRGKAVS VDAKLVKPGD HFEVTGIATY QADVEVSFEL
     ELEAGTSLLE KAEAFDPAYD DDAQKLCGVK GADARGGVGP FGLWVLASAD LQERTAVFFR
     VFRDGHGKPK VLMCTDPTKS SLSPDLYKPT FAGFVDADIS SGKITLRSLI DRSVVESFGA
     GGKTCILSRV YPSIAVGKDA HLYVFNNGEV DVTVSGLTAW EMKKPLMNGA