ID   INVB_ZYMMA              Reviewed;         413 AA.
AC   F8DT27; Q5NQK5; Q60115; Q60117; Q60125;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Extracellular sucrase;
DE            EC=3.2.1.26;
DE   AltName: Full=Beta-fructofuranosidase;
DE   AltName: Full=Invertase;
DE   AltName: Full=Protein B46;
DE   AltName: Full=Saccharase;
GN   Name=sacC; Synonyms=invB, sucE3; OrderedLocusNames=Zmob_0915;
OS   Zymomonas mobilis subsp. mobilis (strain ATCC 10988 / DSM 424 / LMG 404 /
OS   NCIMB 8938 / NRRL B-806 / ZM1).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Zymomonadaceae; Zymomonas.
OX   NCBI_TaxID=555217;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=8086457; DOI=10.1016/0167-4781(94)90262-3;
RA   Song K.B., Lee S.K., Joo H.K., Rhee S.-K.;
RT   "Nucleotide and derived amino acid sequences of an extracellular sucrase
RT   gene (invB) of Zymomonas mobilis ZM1 (ATCC10988).";
RL   Biochim. Biophys. Acta 1219:163-166(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, AND SUBCELLULAR
RP   LOCATION.
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=7778976; DOI=10.1007/bf00305353;
RA   Kannan R., Mukundan G., Ait-Abdelkader N., Augier-Magro V., Baratti J.,
RA   Gunasekaran P.;
RT   "Molecular cloning and characterization of the extracellular sucrase gene
RT   (sacC) of Zymomonas mobilis.";
RL   Arch. Microbiol. 163:195-204(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10988 / DSM 424 / CCUG 17860 / LMG 404 / NCIMB 8938 / NRRL
RC   B-806 / ZM1;
RX   PubMed=21725006; DOI=10.1128/jb.05395-11;
RA   Pappas K.M., Kouvelis V.N., Saunders E., Brettin T.S., Bruce D., Detter C.,
RA   Balakireva M., Han C.S., Savvakis G., Kyrpides N.C., Typas M.A.;
RT   "Genome sequence of the ethanol-producing Zymomonas mobilis subsp. mobilis
RT   lectotype strain ATCC 10988.";
RL   J. Bacteriol. 193:5051-5052(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC         Evidence={ECO:0000269|PubMed:7778976};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:7778976,
CC       ECO:0000269|PubMed:8086457}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
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DR   EMBL; AF081588; AAA61488.1; -; Genomic_DNA.
DR   EMBL; L33403; AAC36942.1; -; Genomic_DNA.
DR   EMBL; CP002850; AEH62750.1; -; Genomic_DNA.
DR   PIR; JC2520; JC2520.
DR   PIR; S47527; S47527.
DR   RefSeq; WP_012817354.1; NC_017262.1.
DR   AlphaFoldDB; F8DT27; -.
DR   SMR; F8DT27; -.
DR   STRING; 555217.Zmob_0915; -.
DR   CAZy; GH68; Glycoside Hydrolase Family 68.
DR   EnsemblBacteria; AEH62750; AEH62750; Zmob_0915.
DR   GeneID; 58026223; -.
DR   KEGG; zmm:Zmob_0915; -.
DR   eggNOG; COG1621; Bacteria.
DR   HOGENOM; CLU_031862_1_0_5; -.
DR   OMA; NGWSVIV; -.
DR   Proteomes; UP000001494; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050053; F:levansucrase activity; IEA:InterPro.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycosidase; Hydrolase; Secreted.
FT   CHAIN           1..413
FT                   /note="Extracellular sucrase"
FT                   /id="PRO_0000414234"
FT   ACT_SITE        44
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   ACT_SITE        276
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   SITE            192
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   CONFLICT        85
FT                   /note="G -> A (in Ref. 2; AAC36942)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        404..407
FT                   /note="PVWP -> LGMA (in Ref. 2; AAC36942)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   413 AA;  46100 MW;  BACC12D167332E47 CRC64;
     MFNFNASRWT RAQAMKVNKF DLTTSMPEIG TDFPIMRDDL WLWDTWPLRD INGNPVSFKG
     WNVIFSLVAD RNIPWNDRHS HARIGYFYSK DGKSWVYGGH LLQESANTRT AEWSGGTIMA
     PGSRNQVETF FTSTLFDKNG VREAVAAVTK GRIYADSEGV WFKGFDQSTD LFQADGLFYQ
     NYAENNLWNF RDPHVFINPE DGETYALFEA NVATVRGEDD IGEDEIGPVP ANTVVPKDAN
     LCSASIGIAR CLSPDRTEWE LLPPLLTAFG VNDQMERPHV IFQNGLTYLF TISHDSTYAD
     GLTGSDGLYG FVSENGIFGP YEPLNGSGLV LGGPASQPTE AYAHYIMNNG LVESFINEII
     DPKSGKVIAG GSLAPTVRVE LQGHETFATE VFDYGYIPAS YAWPVWPFPD RRK