ID   INVB_ZYMMO              Reviewed;         413 AA.
AC   P0DJA4; Q5NQK5; Q60115; Q60117; Q60125;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 45.
DE   RecName: Full=Extracellular sucrase;
DE            EC=3.2.1.26;
DE   AltName: Full=Beta-fructofuranosidase;
DE   AltName: Full=Invertase;
DE   AltName: Full=Protein B46;
DE   AltName: Full=Saccharase;
GN   Name=sacC; Synonyms=invB, sucE3; OrderedLocusNames=ZMO0375;
OS   Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Zymomonadaceae; Zymomonas.
OX   NCBI_TaxID=264203;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29191 / DSM 3580 / JCM 10190 / CECT 560 / NBRC 13756 / NCIMB
RC   11199 / NRRL B-4490 / ZM6;
RX   PubMed=7766026; DOI=10.1271/bbb.59.289;
RA   Kyono K., Yanase H., Tonomura K., Kawasaki H., Sakai T.;
RT   "Cloning and characterization of Zymomonas mobilis genes encoding
RT   extracellular levansucrase and invertase.";
RL   Biosci. Biotechnol. Biochem. 59:289-293(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 31821 / ZM4 / CP4;
RA   Ahn J.Y., Kang H.S.;
RT   "Sequence analysis of 44B6 fosmid clone of Zymomonas mobilis ZM4.";
RL   Submitted (OCT-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31821 / ZM4 / CP4;
RX   PubMed=15592456; DOI=10.1038/nbt1045;
RA   Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H.,
RA   Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J.,
RA   Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y., Kang H.L., Lee S.Y., Lee K.J.,
RA   Kang H.S.;
RT   "The genome sequence of the ethanologenic bacterium Zymomonas mobilis
RT   ZM4.";
RL   Nat. Biotechnol. 23:63-68(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC         residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 68 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAV88999.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; D17524; BAA04476.1; -; Genomic_DNA.
DR   EMBL; AF313764; AAG29871.1; -; Genomic_DNA.
DR   EMBL; AE008692; AAV88999.2; ALT_INIT; Genomic_DNA.
DR   PIR; JC2520; JC2520.
DR   RefSeq; WP_012817354.1; NZ_CP035711.1.
DR   AlphaFoldDB; P0DJA4; -.
DR   SMR; P0DJA4; -.
DR   STRING; 264203.ZMO0375; -.
DR   CAZy; GH68; Glycoside Hydrolase Family 68.
DR   EnsemblBacteria; AAV88999; AAV88999; ZMO0375.
DR   GeneID; 58026223; -.
DR   KEGG; zmo:ZMO0375; -.
DR   eggNOG; COG1621; Bacteria.
DR   HOGENOM; CLU_031862_1_0_5; -.
DR   OrthoDB; 1622507at2; -.
DR   Proteomes; UP000001173; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0050053; F:levansucrase activity; IEA:InterPro.
DR   GO; GO:0009758; P:carbohydrate utilization; IEA:InterPro.
DR   GO; GO:0008152; P:metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd08997; GH68; 1.
DR   Gene3D; 2.115.10.20; -; 1.
DR   InterPro; IPR003469; Glyco_hydro_68.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   Pfam; PF02435; Glyco_hydro_68; 1.
DR   SUPFAM; SSF75005; SSF75005; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Reference proteome; Secreted.
FT   CHAIN           1..413
FT                   /note="Extracellular sucrase"
FT                   /id="PRO_0000057722"
FT   ACT_SITE        44
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   ACT_SITE        276
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   SITE            192
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q74K42"
FT   CONFLICT        105
FT                   /note="S -> L (in Ref. 1; BAA04476)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        108
FT                   /note="T -> S (in Ref. 1; BAA04476)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   413 AA;  46100 MW;  BACC12D167332E47 CRC64;
     MFNFNASRWT RAQAMKVNKF DLTTSMPEIG TDFPIMRDDL WLWDTWPLRD INGNPVSFKG
     WNVIFSLVAD RNIPWNDRHS HARIGYFYSK DGKSWVYGGH LLQESANTRT AEWSGGTIMA
     PGSRNQVETF FTSTLFDKNG VREAVAAVTK GRIYADSEGV WFKGFDQSTD LFQADGLFYQ
     NYAENNLWNF RDPHVFINPE DGETYALFEA NVATVRGEDD IGEDEIGPVP ANTVVPKDAN
     LCSASIGIAR CLSPDRTEWE LLPPLLTAFG VNDQMERPHV IFQNGLTYLF TISHDSTYAD
     GLTGSDGLYG FVSENGIFGP YEPLNGSGLV LGGPASQPTE AYAHYIMNNG LVESFINEII
     DPKSGKVIAG GSLAPTVRVE LQGHETFATE VFDYGYIPAS YAWPVWPFPD RRK