INVC_ARATH
ID INVC_ARATH Reviewed; 664 AA.
AC B9DFA8; Q0WWN9; Q7Y209; Q9C8Z1;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 24-MAR-2009, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Alkaline/neutral invertase C, mitochondrial {ECO:0000305};
DE Short=A/N-INVC {ECO:0000303|PubMed:21441406};
DE EC=3.2.1.26 {ECO:0000269|PubMed:23135328};
DE Flags: Precursor;
GN Name=INVC {ECO:0000303|PubMed:21441406};
GN OrderedLocusNames=At3g06500 {ECO:0000312|Araport:AT3G06500};
GN ORFNames=F5E6.17 {ECO:0000312|EMBL:AAG51337.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=21441406; DOI=10.1093/jxb/err069;
RA Xiang L., Le Roy K., Bolouri-Moghaddam M.R., Vanhaecke M., Lammens W.,
RA Rolland F., Van den Ende W.;
RT "Exploring the neutral invertase-oxidative stress defence connection in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 62:3849-3862(2011).
RN [7]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=23135328; DOI=10.1007/s00425-012-1794-8;
RA Martin M.L., Lechner L., Zabaleta E.J., Salerno G.L.;
RT "A mitochondrial alkaline/neutral invertase isoform (A/N-InvC) functions in
RT developmental energy-demanding processes in Arabidopsis.";
RL Planta 237:813-822(2013).
CC -!- FUNCTION: Mitochondrial invertase that cleaves sucrose into glucose and
CC fructose and is involved in the regulation of aerial tissue development
CC and floral transition. May be modulating hormone balance in relation to
CC the radicle emergence. {ECO:0000269|PubMed:23135328}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000269|PubMed:23135328};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 6.0. {ECO:0000269|PubMed:23135328};
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:23135328}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, roots and flowers.
CC {ECO:0000269|PubMed:23135328}.
CC -!- DISRUPTION PHENOTYPE: Delayed germination time, reduced plant growth,
CC delayed flowering and reduced oxygen consumption in the dark.
CC {ECO:0000269|PubMed:23135328}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 100 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51337.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC020580; AAG51337.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; AEE74403.1; -; Genomic_DNA.
DR EMBL; BT008650; AAP40464.1; -; mRNA.
DR EMBL; AK316698; BAH19425.1; -; mRNA.
DR EMBL; AK226304; BAE98459.1; -; mRNA.
DR RefSeq; NP_187302.2; NM_111526.4.
DR AlphaFoldDB; B9DFA8; -.
DR SMR; B9DFA8; -.
DR STRING; 3702.AT3G06500.1; -.
DR CAZy; GH100; Glycoside Hydrolase Family 100.
DR iPTMnet; B9DFA8; -.
DR PaxDb; B9DFA8; -.
DR PRIDE; B9DFA8; -.
DR ProteomicsDB; 247217; -.
DR EnsemblPlants; AT3G06500.1; AT3G06500.1; AT3G06500.
DR GeneID; 819828; -.
DR Gramene; AT3G06500.1; AT3G06500.1; AT3G06500.
DR KEGG; ath:AT3G06500; -.
DR Araport; AT3G06500; -.
DR TAIR; locus:2084329; AT3G06500.
DR eggNOG; ENOG502QT23; Eukaryota.
DR HOGENOM; CLU_020846_0_0_1; -.
DR InParanoid; B9DFA8; -.
DR OMA; YPTMLVP; -.
DR OrthoDB; 373994at2759; -.
DR PRO; PR:B9DFA8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; B9DFA8; baseline and differential.
DR Genevisible; B9DFA8; AT.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0033926; F:glycopeptide alpha-N-acetylgalactosaminidase activity; IEA:InterPro.
DR GO; GO:0004575; F:sucrose alpha-glucosidase activity; IDA:TAIR.
DR GO; GO:0007623; P:circadian rhythm; IEP:TAIR.
DR GO; GO:0010029; P:regulation of seed germination; IMP:TAIR.
DR GO; GO:0048510; P:regulation of timing of transition from vegetative to reproductive phase; IMP:TAIR.
DR GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR024746; Glyco_hydro_100.
DR PANTHER; PTHR31916; PTHR31916; 1.
DR Pfam; PF12899; Glyco_hydro_100; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism; Glycosidase; Hydrolase; Mitochondrion;
KW Phosphoprotein; Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000305"
FT CHAIN ?..664
FT /note="Alkaline/neutral invertase C, mitochondrial"
FT /evidence="ECO:0000305"
FT /id="PRO_0000431499"
FT MOD_RES 41
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT MOD_RES 125
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT MOD_RES 657
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT CONFLICT 9
FT /note="V -> I (in Ref. 5; BAE98459)"
FT /evidence="ECO:0000305"
FT CONFLICT 575
FT /note="V -> F (in Ref. 3; AAP40464)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 664 AA; 74722 MW; 14E8108C181727D0 CRC64;
MNSRSCICVS AMKPCCRFLI SFRSSSLFGF SPPNSGKFIN SSKLHCTKID SRSIRSGIHC
RRIVLDRNAF CDSDSISWGG GGSRVLRARG SSRGRGRGVL VIPHVASDFR NYSTSSLDSH
VNDKSFESMF VKPLVFKEVE KTEGIPKRER GNVGGGKDAN FGNVGVRKET ERCLSQTEVE
KEAWKLLRGA VVNYCGFPVG TVAANDPGDT QTLNYDQVFI RDFVPSAYAF MLDGEGEIVR
NFLLHTLQLQ SWEKTVDCHS PGPGLMPASF KVKSAPLEGN DGSFEEFLDP DFGGSAIGRV
SPVDSGLWWI ILLRAYGKLT GDYTLQERID VQTGIKLILK LCLADGFDMF PTLLVTDGSC
MVDRRMGIHG HPLEIQALFY SALRCAREML IVNDGTKSLV TAVNNRLSAL SFHIREYYWV
DIKKINEIYR YNTEEYSADA TNKFNIYPEQ IPTWLVDWIP DKGGYFIGNL QPAHMDFRFF
TLGNLWAVIS SLGNQEQNEG VMTLIEEKWD DLVANMPLKI CFPALEKDEW RIITGSDPKN
TPWSYHNGGS WPTLLWQFTL ACIKMGKLEL AKKAVAVAEK RLKEDEWPEY YDTKSGRFVG
KQSRLYQTWT IAGFLAAKKL IEQPEKASLL FWEEDYQLLE TCVCGLSKSS GRKNKCSRFT
PPRS
