INVD_ARATH
ID INVD_ARATH Reviewed; 534 AA.
AC F4I2X9; O80556; Q8L9K0;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 62.
DE RecName: Full=Probable alkaline/neutral invertase D {ECO:0000305};
DE Short=A/N-INVD {ECO:0000303|PubMed:21441406};
DE EC=3.2.1.26 {ECO:0000250|UniProtKB:Q9FXA8};
GN Name=INVD {ECO:0000303|PubMed:21441406};
GN OrderedLocusNames=At1g22650 {ECO:0000312|Araport:AT1G22650};
GN ORFNames=T22J18.18 {ECO:0000312|EMBL:AAC25521.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=21441406; DOI=10.1093/jxb/err069;
RA Xiang L., Le Roy K., Bolouri-Moghaddam M.R., Vanhaecke M., Lammens W.,
RA Rolland F., Van den Ende W.;
RT "Exploring the neutral invertase-oxidative stress defence connection in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 62:3849-3862(2011).
CC -!- FUNCTION: Invertase that cleaves sucrose into glucose and fructose.
CC {ECO:0000250|UniProtKB:Q9LQF2}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000250|UniProtKB:Q9FXA8};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 100 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC25521.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003979; AAC25521.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30264.1; -; Genomic_DNA.
DR EMBL; AY088388; AAM65926.1; -; mRNA.
DR PIR; T00779; T00779.
DR RefSeq; NP_564177.1; NM_102112.4.
DR AlphaFoldDB; F4I2X9; -.
DR SMR; F4I2X9; -.
DR STRING; 3702.AT1G22650.1; -.
DR CAZy; GH100; Glycoside Hydrolase Family 100.
DR iPTMnet; F4I2X9; -.
DR PaxDb; F4I2X9; -.
DR PRIDE; F4I2X9; -.
DR ProteomicsDB; 248512; -.
DR EnsemblPlants; AT1G22650.1; AT1G22650.1; AT1G22650.
DR GeneID; 838871; -.
DR Gramene; AT1G22650.1; AT1G22650.1; AT1G22650.
DR KEGG; ath:AT1G22650; -.
DR Araport; AT1G22650; -.
DR TAIR; locus:2199690; AT1G22650.
DR eggNOG; ENOG502QPS0; Eukaryota.
DR HOGENOM; CLU_020846_1_1_1; -.
DR InParanoid; F4I2X9; -.
DR OMA; GRPDFRF; -.
DR OrthoDB; 344496at2759; -.
DR PRO; PR:F4I2X9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; F4I2X9; baseline and differential.
DR Genevisible; F4I2X9; AT.
DR GO; GO:0033926; F:glycopeptide alpha-N-acetylgalactosaminidase activity; IEA:InterPro.
DR GO; GO:0004575; F:sucrose alpha-glucosidase activity; IBA:GO_Central.
DR GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR024746; Glyco_hydro_100.
DR PANTHER; PTHR31916; PTHR31916; 1.
DR Pfam; PF12899; Glyco_hydro_100; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Glycosidase; Hydrolase; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..534
FT /note="Probable alkaline/neutral invertase D"
FT /id="PRO_0000431500"
FT MOD_RES 7
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT MOD_RES 37
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT MOD_RES 55
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT MOD_RES 532
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
FT CONFLICT 400
FT /note="E -> A (in Ref. 3; AAM65926)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 534 AA; 60868 MW; 1D5451CD48D492DA CRC64;
MEGVNSSSSI SDLDELARLL DRPRVNIERK RSFDERSFSE MGIFDNVNSP GGWETPVSSA
RNSFEPHPMV AEAWDALRRS LVYFRGQPVG TIAAYDHATE EVLNYDQVFV RDFVPSALAF
LMNGEPDIVK NFLLKTIQIQ GREKRIDRFK LGEGAMPASF KVIHDPIKET DSINADFGES
AIGRVAPVDS GFWWIILLRA YTKSTGDTSL AETSECQKGM RLILSLCLSE GFDTFPTLLC
ADGCSMIDRR MGVYGYPIEI QALFFMALRS AMSMLKHDAE GKEFMERIVK RLHALSFHMR
SYFWLDFQQL NDIYRYKTEE YSHTAVNKFN VIPDSIPEWV FDFMPLRGGY FIGNVSPARM
DFRWFALGNC VAILASLATP EQSASIMDLI EERWEELVGE MPVKICHPAI ESHEWRIVTG
CDPKNTRWSY HNGGSWPVLL WLLTAACIKT GRPQIARRAI DLAEARLLKD GWPEYYDGKS
GRFIGKQARK FQTWSIAGYL VAKMLLEDPS HLGMISLEED KQTKPVIKRS YSWT
