INVH_ARATH
ID INVH_ARATH Reviewed; 633 AA.
AC Q84JL5; F4J8I9; Q9M9L4;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Probable alkaline/neutral invertase A, chloroplastic {ECO:0000305};
DE Short=A/N-INVA {ECO:0000303|PubMed:21441406};
DE EC=3.2.1.26 {ECO:0000250|UniProtKB:Q9FXA8};
DE Flags: Precursor;
GN Name=INVH {ECO:0000303|PubMed:21441406};
GN OrderedLocusNames=At3g05820 {ECO:0000312|Araport:AT3G05820};
GN ORFNames=F10A16.11 {ECO:0000312|EMBL:AAF26084.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=21441406; DOI=10.1093/jxb/err069;
RA Xiang L., Le Roy K., Bolouri-Moghaddam M.R., Vanhaecke M., Lammens W.,
RA Rolland F., Van den Ende W.;
RT "Exploring the neutral invertase-oxidative stress defence connection in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 62:3849-3862(2011).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=18034262; DOI=10.1007/s00425-007-0657-1;
RA Vargas W.A., Pontis H.G., Salerno G.L.;
RT "New insights on sucrose metabolism: evidence for an active A/N-Inv in
RT chloroplasts uncovers a novel component of the intracellular carbon
RT trafficking.";
RL Planta 227:795-807(2008).
CC -!- FUNCTION: Chloroplastic invertase that cleaves sucrose into glucose and
CC fructose and may participate in the carbon flux between the cytosol and
CC plastids in leaves. {ECO:0000250|UniProtKB:Q9FK88}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000250|UniProtKB:Q9FXA8};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers.
CC {ECO:0000269|PubMed:18034262}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 100 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26084.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC012393; AAF26084.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002686; ANM64512.1; -; Genomic_DNA.
DR EMBL; BT004140; AAO42161.1; -; mRNA.
DR EMBL; BT005695; AAO64115.1; -; mRNA.
DR RefSeq; NP_001326532.1; NM_001337613.1.
DR AlphaFoldDB; Q84JL5; -.
DR SMR; Q84JL5; -.
DR STRING; 3702.AT3G05820.1; -.
DR CAZy; GH100; Glycoside Hydrolase Family 100.
DR PaxDb; Q84JL5; -.
DR PRIDE; Q84JL5; -.
DR ProteomicsDB; 248472; -.
DR EnsemblPlants; AT3G05820.2; AT3G05820.2; AT3G05820.
DR GeneID; 819751; -.
DR Gramene; AT3G05820.2; AT3G05820.2; AT3G05820.
DR KEGG; ath:AT3G05820; -.
DR Araport; AT3G05820; -.
DR TAIR; locus:2074434; AT3G05820.
DR eggNOG; ENOG502QT23; Eukaryota.
DR HOGENOM; CLU_020846_0_0_1; -.
DR OMA; WIPESHR; -.
DR OrthoDB; 373994at2759; -.
DR PhylomeDB; Q84JL5; -.
DR PRO; PR:Q84JL5; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q84JL5; baseline and differential.
DR Genevisible; Q84JL5; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0033926; F:glycopeptide alpha-N-acetylgalactosaminidase activity; IEA:InterPro.
DR GO; GO:0004575; F:sucrose alpha-glucosidase activity; IBA:GO_Central.
DR GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR024746; Glyco_hydro_100.
DR PANTHER; PTHR31916; PTHR31916; 1.
DR Pfam; PF12899; Glyco_hydro_100; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 2: Evidence at transcript level;
KW Carbohydrate metabolism; Chloroplast; Glycosidase; Hydrolase;
KW Phosphoprotein; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..71
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 72..633
FT /note="Probable alkaline/neutral invertase A,
FT chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000431503"
FT MOD_RES 623
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LQF2"
SQ SEQUENCE 633 AA; 71920 MW; 93E100F9596DA92C CRC64;
MNAITFLGNS TMIPSQCILR AFTRISPSKY IRDTSFRSYP SRFSSCINQY RNADSDRIIR
PTNAVPFCTD RQSSVTAQVV SEARSHSAST TCANDTTLDQ IYTKNGLNVK PLVVERLKRD
EKDEEAVNED EEGVKRDGFE GVKCNDVEEE AWRLLRDSIV TYCDSPVGTV AAKDPTDTTP
SNYDQVFIRD FVPSALAFLL KGESEIVRNF LLHTLQLQSW EKTVDCYSPG QGLMPASFKV
RTLPLEEDKF EEVLDPDFGE AAIGRVAPVD SGLWWIILLR AYGKITGDYS LQERIDVQTG
IKMIANLCLA DGFDMFPTLL VTDGSCMIDR RMGIHGHPLE IQALFYSALR SSREMITVND
SSKNIIKTIS NRLSALSFHI RENYWVDKNK INEIYRYKTE EYSMDATNKF NIYPEQVSPW
LMDWVPESPD SGFLIGNLQP AHMDFRFFTL GNLWSIISSL GTPKQNQAIL NLVEEKWDDL
VGHMPLKICY PALESSEWHI ITGSDPKNTP WSYHNGGSWP TLLWQFTLAC IKMGRPELAE
KAVTLAEKRL QADRWPEYYD TRDGKFIGKQ SRLYQTWTIA GFLTSKQLLQ NPEIASSLFW
EEDLELLESC VCVLTKSGRK KCSRAAAKSQ ILI
