INX4_DROME
ID INX4_DROME Reviewed; 367 AA.
AC Q9VRX6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Innexin inx4;
DE Short=Innexin-4;
DE AltName: Full=Protein zero population growth;
GN Name=zpg; Synonyms=inx4; ORFNames=CG10125;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Ovary;
RX PubMed=11960713; DOI=10.1016/s0925-4773(02)00025-4;
RA Stebbings L.A., Todman M.G., Phillips R., Greer C.E., Tam J., Phelan P.,
RA Jacobs K., Bacon J.P., Davies J.A.;
RT "Gap junctions in Drosophila: developmental expression of the entire
RT innexin gene family.";
RL Mech. Dev. 113:197-205(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RC TISSUE=Ovary;
RX PubMed=11973283; DOI=10.1242/dev.129.10.2529;
RA Tazuke S.I., Schulz C., Gilboa L., Fogarty M., Mahowald A.P., Guichet A.,
RA Ephrussi A., Wood C.G., Lehmann R., Fuller M.T.;
RT "A germline-specific gap junction protein required for survival of
RT differentiating early germ cells.";
RL Development 129:2529-2539(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Structural component of the gap junctions in germline cells.
CC Required for differentiation and survival of germline cysts in females
CC and of spermatogonia in males; gap junctional communication between
CC spermatogonia and somatic cyst cells may be required for normal
CC differentiation and survival of spermatogonia.
CC {ECO:0000269|PubMed:11960713, ECO:0000269|PubMed:11973283}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11973283};
CC Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00351,
CC ECO:0000269|PubMed:11973283}. Cell junction, gap junction
CC {ECO:0000269|PubMed:11973283}. Note=Concentrated at the interface
CC between germline and somatic support cells in spermatogonia, early
CC spermatocytes and germ cells in the ovary.
CC -!- TISSUE SPECIFICITY: Expressed in nurse cells and oocyte during
CC oogenesis. Uniform expression in imaginal wing disk and low expression
CC in developing imaginal CNS. Expressed in embryonic pole cells and
CC primordial germ cells. {ECO:0000269|PubMed:11960713,
CC ECO:0000269|PubMed:11973283}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. First
CC seen at the embryonic syncytial blastoderm stage.
CC {ECO:0000269|PubMed:11973283}.
CC -!- SIMILARITY: Belongs to the pannexin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00351}.
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DR EMBL; AF271718; AAL36976.1; -; mRNA.
DR EMBL; AE014296; AAF50655.1; -; Genomic_DNA.
DR EMBL; AY094856; AAM11209.1; -; mRNA.
DR RefSeq; NP_001261478.1; NM_001274549.1.
DR RefSeq; NP_648049.1; NM_139792.3.
DR AlphaFoldDB; Q9VRX6; -.
DR BioGRID; 75539; 4.
DR DIP; DIP-17426N; -.
DR STRING; 7227.FBpp0303218; -.
DR TCDB; 1.A.25.1.8; the gap junction-forming innexin (innexin) family.
DR PaxDb; Q9VRX6; -.
DR DNASU; 251414; -.
DR EnsemblMetazoa; FBtr0076983; FBpp0076692; FBgn0024177.
DR EnsemblMetazoa; FBtr0330185; FBpp0303218; FBgn0024177.
DR GeneID; 251414; -.
DR KEGG; dme:Dmel_CG10125; -.
DR CTD; 251414; -.
DR FlyBase; FBgn0024177; zpg.
DR VEuPathDB; VectorBase:FBgn0024177; -.
DR eggNOG; ENOG502QR27; Eukaryota.
DR GeneTree; ENSGT00530000064205; -.
DR HOGENOM; CLU_035763_1_1_1; -.
DR InParanoid; Q9VRX6; -.
DR OMA; YQWVVLV; -.
DR OrthoDB; 738314at2759; -.
DR PhylomeDB; Q9VRX6; -.
DR BioGRID-ORCS; 251414; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 251414; -.
DR PRO; PR:Q9VRX6; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0024177; Expressed in adult abdomen and 13 other tissues.
DR ExpressionAtlas; Q9VRX6; baseline and differential.
DR Genevisible; Q9VRX6; DM.
DR GO; GO:0005921; C:gap junction; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0005243; F:gap junction channel activity; IBA:GO_Central.
DR GO; GO:0055077; F:gap junction hemi-channel activity; NAS:UniProtKB.
DR GO; GO:0007281; P:germ cell development; IMP:FlyBase.
DR GO; GO:0010496; P:intercellular transport; ISS:FlyBase.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0036098; P:male germ-line stem cell population maintenance; IMP:FlyBase.
DR GO; GO:0007602; P:phototransduction; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR InterPro; IPR000990; Innexin.
DR PANTHER; PTHR11893; PTHR11893; 1.
DR Pfam; PF00876; Innexin; 1.
DR PRINTS; PR01262; INNEXIN.
DR PROSITE; PS51013; PANNEXIN; 1.
PE 2: Evidence at transcript level;
KW Cell junction; Cell membrane; Gap junction; Ion channel; Ion transport;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..367
FT /note="Innexin inx4"
FT /id="PRO_0000208499"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TOPO_DOM 43..110
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TOPO_DOM 132..186
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TOPO_DOM 208..268
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TOPO_DOM 290..367
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 367 AA; 42784 MW; B984A4035DA7CF3E CRC64;
MYAAVKPLSK YLQFKSVHIY DAIFTLHSKV TVALLLACTF LLSSKQYFGD PIQCFGDKDM
DYVHAFCWIY GAYVSDNVTV TPLRNGAAQC RPDAVSKVVP PENRNYITYY QWVVLVLLLE
SFVFYMPAFL WKIWEGGRLK HLCDDFHKMA VCKDKSRTHL RVLVNYFSSD YKETHFRYFV
SYVFCEILNL SISILNFLLL DVFFGGFWGR YRNALLSLYN GDYNQWNIIT MAVFPKCAKC
EMYKGGPSGS SNIYDYLCLL PLNILNEKIF AFLWIWFILV AMLISLKFLY RLATVLYPGM
RLQLLRARAR FMPKKHLQVA LRNCSFGDWF VLMRVGNNIS PELFRKLLEE LYEAQSLIKI
PPGADKI
