ID   IOD1_PIG                Reviewed;         249 AA.
AC   Q6QN13;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   26-FEB-2008, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Type I iodothyronine deiodinase;
DE            EC=1.21.99.4;
DE   AltName: Full=5DI;
DE   AltName: Full=DIOI;
DE   AltName: Full=Type 1 DI;
DE   AltName: Full=Type-I 5'-deiodinase;
GN   Name=DIO1;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   STRAIN=Landrace X Yorkshire;
RX   PubMed=15192045; DOI=10.1210/en.2004-0356;
RA   Wassen F.W.J.S., Klootwijk W., Kaptein E., Duncker D.J., Visser T.J.,
RA   Kuiper G.G.J.M.;
RT   "Characteristics and thyroid state-dependent regulation of iodothyronine
RT   deiodinases in pigs.";
RL   Endocrinology 145:4251-4263(2004).
CC   -!- FUNCTION: Responsible for the deiodination of T4 (3,5,3',5'-
CC       tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2
CC       (3,3'-diiodothyronine). {ECO:0000269|PubMed:15192045}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3,3',5-triiodo-L-thyronine + A + H(+) + iodide = AH2 + L-
CC         thyroxine; Xref=Rhea:RHEA:19745, ChEBI:CHEBI:13193,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16382, ChEBI:CHEBI:17499,
CC         ChEBI:CHEBI:58448, ChEBI:CHEBI:533015; EC=1.21.99.4;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10107};
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the iodothyronine deiodinase family.
CC       {ECO:0000305}.
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DR   EMBL; AY533206; AAS48447.1; -; mRNA.
DR   RefSeq; NP_001001627.1; NM_001001627.1.
DR   STRING; 9823.ENSSSCP00000025038; -.
DR   PaxDb; Q6QN13; -.
DR   Ensembl; ENSSSCT00000026326; ENSSSCP00000025038; ENSSSCG00000028896.
DR   Ensembl; ENSSSCT00005052219; ENSSSCP00005032283; ENSSSCG00005032671.
DR   Ensembl; ENSSSCT00025013973; ENSSSCP00025005431; ENSSSCG00025010634.
DR   Ensembl; ENSSSCT00030094739; ENSSSCP00030043644; ENSSSCG00030067761.
DR   Ensembl; ENSSSCT00035068667; ENSSSCP00035027792; ENSSSCG00035051563.
DR   Ensembl; ENSSSCT00040034925; ENSSSCP00040014474; ENSSSCG00040026128.
DR   Ensembl; ENSSSCT00045039841; ENSSSCP00045027739; ENSSSCG00045023320.
DR   Ensembl; ENSSSCT00050035418; ENSSSCP00050014729; ENSSSCG00050026315.
DR   Ensembl; ENSSSCT00055054455; ENSSSCP00055043435; ENSSSCG00055027536.
DR   Ensembl; ENSSSCT00065107599; ENSSSCP00065047982; ENSSSCG00065077758.
DR   Ensembl; ENSSSCT00070060565; ENSSSCP00070051609; ENSSSCG00070030119.
DR   GeneID; 414380; -.
DR   KEGG; ssc:414380; -.
DR   CTD; 1733; -.
DR   VGNC; VGNC:87304; DIO1.
DR   eggNOG; ENOG502QUGZ; Eukaryota.
DR   GeneTree; ENSGT00940000154482; -.
DR   HOGENOM; CLU_089345_2_0_1; -.
DR   InParanoid; Q6QN13; -.
DR   OMA; SFGSCTX; -.
DR   OrthoDB; 977776at2759; -.
DR   SABIO-RK; Q6QN13; -.
DR   Proteomes; UP000008227; Chromosome 6.
DR   Proteomes; UP000314985; Chromosome 6.
DR   Bgee; ENSSSCG00000028896; Expressed in adult mammalian kidney and 18 other tissues.
DR   Genevisible; Q6QN13; SS.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004800; F:thyroxine 5'-deiodinase activity; IBA:GO_Central.
DR   GO; GO:0006520; P:cellular amino acid metabolic process; IEA:Ensembl.
DR   GO; GO:0042446; P:hormone biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0042403; P:thyroid hormone metabolic process; IBA:GO_Central.
DR   InterPro; IPR000643; Iodothyronine_deiodinase.
DR   InterPro; IPR008261; Iodothyronine_deiodinase_AS.
DR   InterPro; IPR027252; Iodothyronine_deiodinase_I/III.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   PANTHER; PTHR11781; PTHR11781; 1.
DR   Pfam; PF00837; T4_deiodinase; 1.
DR   PIRSF; PIRSF001330; IOD; 1.
DR   PIRSF; PIRSF500144; IODI_III; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   PROSITE; PS01205; T4_DEIODINASE; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; Oxidoreductase; Reference proteome;
KW   Selenocysteine; Thyroid hormones biosynthesis; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..249
FT                   /note="Type I iodothyronine deiodinase"
FT                   /id="PRO_0000318638"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        126
FT   NON_STD         126
FT                   /note="Selenocysteine"
SQ   SEQUENCE   249 AA;  28888 MW;  5D805F25F64B1465 CRC64;
     MELPLPGLWL KRLWVLFQVA LHVAMGKVLM TLFPGRVKQD ILAMSQKTGM AKNPHFSHEN
     WIPTFFSAQY FWFVLKVRWQ RLEDKTEEGG LAPNCPVVSL SGQRCHIWDF MQGNRPLVLN
     FGSCTUPSFI FKFDQFKRLI EDFSSIADFL IIYIEEAHAS DGWAFKNNVD IKNHQNLQDR
     LRAAHLLLDR SPQCPVVVDT MKNQSSRLYA ALPERLYVLQ AGRILYKGKP GPWNYHPEEV
     RAVLEKLHS