IOD2_MOUSE
ID IOD2_MOUSE Reviewed; 266 AA.
AC Q9Z1Y9; Q05A70; Q9JHH1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 4.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Type II iodothyronine deiodinase;
DE EC=1.21.99.4;
DE AltName: Full=5DII;
DE AltName: Full=DIOII;
DE AltName: Full=Type 2 DI;
DE AltName: Full=Type-II 5'-deiodinase;
GN Name=Dio2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=9927339; DOI=10.1210/endo.140.2.6678;
RA Davey J.C., Schneider M.J., Becker K.B., Galton V.A.;
RT "Cloning of a 5.8 kb cDNA for a mouse type 2 deiodinase.";
RL Endocrinology 140:1022-1025(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE
RP SPECIFICITY.
RC STRAIN=C3H/HeN; TISSUE=Brain;
RX PubMed=10715551; DOI=10.1016/s0303-7207(99)00249-x;
RA Song S., Sorimachi K., Adachi K., Oka T.;
RT "Biochemical and molecular biological evidence for the presence of type II
RT iodothyronine deiodinase in mouse mammary gland.";
RL Mol. Cell. Endocrinol. 160:173-181(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Cochlea;
RX PubMed=10655523; DOI=10.1073/pnas.97.3.1287;
RA Campos-Barros A., Amma L.L., Faris J.S., Shailam R., Kelley M.W.,
RA Forrest D.;
RT "Type 2 iodothyronine deiodinase expression in the cochlea before the onset
RT of hearing.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:1287-1292(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Responsible for the deiodination of T4 (3,5,3',5'-
CC tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for
CC providing the brain with appropriate levels of T3 during the critical
CC period of development. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3,3',5-triiodo-L-thyronine + A + H(+) + iodide = AH2 + L-
CC thyroxine; Xref=Rhea:RHEA:19745, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16382, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:58448, ChEBI:CHEBI:533015; EC=1.21.99.4;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10107};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=4.4 nM for T4 {ECO:0000269|PubMed:10715551};
CC -!- SUBUNIT: Interacts with USP20 and USP33. Interacts with MARCHF6 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in mammary gland and in brain.
CC {ECO:0000269|PubMed:10715551}.
CC -!- PTM: Ubiquitinated by MARCHF6, leading to its degradation by the
CC proteasome. Deubiquitinated by USP20 and USP33 (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the iodothyronine deiodinase family.
CC {ECO:0000305}.
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DR EMBL; AF096875; AAD11422.1; -; mRNA.
DR EMBL; AF177196; AAD53113.1; -; mRNA.
DR EMBL; AF093137; AAF00069.2; -; mRNA.
DR EMBL; BC125383; AAI25384.1; -; mRNA.
DR EMBL; BC125385; AAI25386.1; -; mRNA.
DR RefSeq; NP_034180.1; NM_010050.3.
DR STRING; 10090.ENSMUSP00000081013; -.
DR PaxDb; Q9Z1Y9; -.
DR PRIDE; Q9Z1Y9; -.
DR ProteomicsDB; 269074; -.
DR Antibodypedia; 47379; 212 antibodies from 31 providers.
DR DNASU; 13371; -.
DR Ensembl; ENSMUST00000082432; ENSMUSP00000081013; ENSMUSG00000007682.
DR GeneID; 13371; -.
DR KEGG; mmu:13371; -.
DR UCSC; uc007okf.1; mouse.
DR CTD; 1734; -.
DR MGI; MGI:1338833; Dio2.
DR VEuPathDB; HostDB:ENSMUSG00000007682; -.
DR eggNOG; ENOG502QS2F; Eukaryota.
DR GeneTree; ENSGT00940000154482; -.
DR HOGENOM; CLU_089345_1_0_1; -.
DR InParanoid; Q9Z1Y9; -.
DR OMA; TPPFISH; -.
DR OrthoDB; 977776at2759; -.
DR PhylomeDB; Q9Z1Y9; -.
DR TreeFam; TF329721; -.
DR BRENDA; 1.21.99.4; 3474.
DR Reactome; R-MMU-350864; Regulation of thyroid hormone activity.
DR SABIO-RK; Q9Z1Y9; -.
DR BioGRID-ORCS; 13371; 0 hits in 75 CRISPR screens.
DR ChiTaRS; Dio2; mouse.
DR PRO; PR:Q9Z1Y9; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; Q9Z1Y9; protein.
DR Bgee; ENSMUSG00000007682; Expressed in median eminence of neurohypophysis and 152 other tissues.
DR Genevisible; Q9Z1Y9; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004800; F:thyroxine 5'-deiodinase activity; IDA:UniProtKB.
DR GO; GO:0033798; F:thyroxine 5-deiodinase activity; ISO:MGI.
DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:MGI.
DR GO; GO:0050873; P:brown fat cell differentiation; IDA:MGI.
DR GO; GO:0044255; P:cellular lipid metabolic process; IMP:MGI.
DR GO; GO:0042446; P:hormone biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0120162; P:positive regulation of cold-induced thermogenesis; IMP:YuBioLab.
DR GO; GO:0009409; P:response to cold; ISO:MGI.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0042404; P:thyroid hormone catabolic process; IMP:MGI.
DR GO; GO:0006590; P:thyroid hormone generation; ISO:MGI.
DR GO; GO:0042403; P:thyroid hormone metabolic process; ISO:MGI.
DR GO; GO:0070460; P:thyroid-stimulating hormone secretion; TAS:UniProtKB.
DR InterPro; IPR000643; Iodothyronine_deiodinase.
DR InterPro; IPR008261; Iodothyronine_deiodinase_AS.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR11781; PTHR11781; 1.
DR Pfam; PF00837; T4_deiodinase; 1.
DR PIRSF; PIRSF001330; IOD; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS01205; T4_DEIODINASE; 1.
PE 1: Evidence at protein level;
KW Membrane; Oxidoreductase; Reference proteome; Selenocysteine;
KW Thyroid hormones biosynthesis; Transmembrane; Transmembrane helix;
KW Ubl conjugation.
FT CHAIN 1..266
FT /note="Type II iodothyronine deiodinase"
FT /id="PRO_0000154318"
FT TRANSMEM 10..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 130
FT NON_STD 130
FT /note="Selenocysteine"
FT NON_STD 263
FT /note="Selenocysteine"
SQ SEQUENCE 266 AA; 29929 MW; B2FD28E171DC5486 CRC64;
MGLLSVDLLI TLQILPVFFS NCLFLALYDS VILLKHVALL LSRSKSTRGE WRRMLTSEGL
RCVWNSFLLD AYKQVKLGED APNSSVVHVS NPESGNNYAS EKTADGAECH LLDFASAERP
LVVNFGSATU PPFTRQLPAF RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSLSFEVK
KHRNQEDRCA AAHQLLERFS LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG
KGPFSYNLQE VRSWLEKNFS KRUILD
