IOD3_PIG
ID IOD3_PIG Reviewed; 305 AA.
AC Q6QN11;
DT 21-FEB-2006, integrated into UniProtKB/Swiss-Prot.
DT 14-MAY-2014, sequence version 3.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Thyroxine 5-deiodinase;
DE EC=1.21.99.3;
DE AltName: Full=5DIII;
DE AltName: Full=DIOIII;
DE AltName: Full=Type 3 DI;
DE AltName: Full=Type III iodothyronine deiodinase;
GN Name=DIO3;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Porcine genome sequencing project;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 28-305, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Landrace X Yorkshire; TISSUE=Brain;
RX PubMed=15192045; DOI=10.1210/en.2004-0356;
RA Wassen F.W.J.S., Klootwijk W., Kaptein E., Duncker D.J., Visser T.J.,
RA Kuiper G.G.J.M.;
RT "Characteristics and thyroid state-dependent regulation of iodothyronine
RT deiodinases in pigs.";
RL Endocrinology 145:4251-4263(2004).
CC -!- FUNCTION: Responsible for the deiodination of T4 (3,5,3',5'-
CC tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3
CC (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2
CC are inactive metabolites. May play a role in preventing premature
CC exposure of developing fetal tissues to adult levels of thyroid
CC hormones. Can regulate circulating fetal thyroid hormone concentrations
CC throughout gestation. Essential role for regulation of thyroid hormone
CC inactivation during embryological development.
CC {ECO:0000269|PubMed:15192045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3,3',5'-triiodo-L-thyronine + A + H(+) + iodide = AH2 + L-
CC thyroxine; Xref=Rhea:RHEA:18897, ChEBI:CHEBI:13193,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16382, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:57261, ChEBI:CHEBI:58448; EC=1.21.99.3;
CC Evidence={ECO:0000250|UniProtKB:P49897};
CC -!- SUBUNIT: Homodimer. May undergo minor heretodimerization with DIO1 and
CC DIO2 (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type II
CC membrane protein {ECO:0000250}. Endosome membrane {ECO:0000250};
CC Single-pass type II membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in brain only.
CC {ECO:0000269|PubMed:15192045}.
CC -!- SIMILARITY: Belongs to the iodothyronine deiodinase family.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-28 is the initiator.
CC {ECO:0000305}.
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DR EMBL; JH116169; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY533208; AAS48449.1; -; mRNA.
DR RefSeq; NP_001001625.2; NM_001001625.2.
DR STRING; 9823.ENSSSCP00000002752; -.
DR PaxDb; Q6QN11; -.
DR Ensembl; ENSSSCT00000053125; ENSSSCP00000052428; ENSSSCG00000036990.
DR Ensembl; ENSSSCT00015038415; ENSSSCP00015015253; ENSSSCG00015028984.
DR Ensembl; ENSSSCT00025041252; ENSSSCP00025017548; ENSSSCG00025030370.
DR Ensembl; ENSSSCT00030053715; ENSSSCP00030024518; ENSSSCG00030038604.
DR Ensembl; ENSSSCT00035073325; ENSSSCP00035029743; ENSSSCG00035054970.
DR Ensembl; ENSSSCT00040105323; ENSSSCP00040048194; ENSSSCG00040075810.
DR Ensembl; ENSSSCT00050004265; ENSSSCP00050001661; ENSSSCG00050003235.
DR Ensembl; ENSSSCT00055037029; ENSSSCP00055029431; ENSSSCG00055018913.
DR Ensembl; ENSSSCT00060007036; ENSSSCP00060002493; ENSSSCG00060005571.
DR Ensembl; ENSSSCT00065051409; ENSSSCP00065022317; ENSSSCG00065037645.
DR GeneID; 414378; -.
DR KEGG; ssc:414378; -.
DR CTD; 1735; -.
DR VGNC; VGNC:87305; DIO3.
DR eggNOG; ENOG502S5FA; Eukaryota.
DR GeneTree; ENSGT00940000154482; -.
DR HOGENOM; CLU_1717459_0_0_1; -.
DR InParanoid; Q6QN11; -.
DR OrthoDB; 977776at2759; -.
DR Reactome; R-SSC-350864; Regulation of thyroid hormone activity.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Unplaced.
DR Bgee; ENSSSCG00000036990; Expressed in Ammon's horn and 16 other tissues.
DR Genevisible; Q6QN11; SS.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004800; F:thyroxine 5'-deiodinase activity; IBA:GO_Central.
DR GO; GO:0033798; F:thyroxine 5-deiodinase activity; IEA:UniProtKB-EC.
DR GO; GO:0042446; P:hormone biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0042403; P:thyroid hormone metabolic process; IBA:GO_Central.
DR InterPro; IPR000643; Iodothyronine_deiodinase.
DR InterPro; IPR008261; Iodothyronine_deiodinase_AS.
DR InterPro; IPR027252; Iodothyronine_deiodinase_I/III.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR11781; PTHR11781; 1.
DR Pfam; PF00837; T4_deiodinase; 1.
DR PIRSF; PIRSF001330; IOD; 1.
DR PIRSF; PIRSF500144; IODI_III; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
DR PROSITE; PS01205; T4_DEIODINASE; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Endosome; Membrane; Oxidoreductase; Reference proteome;
KW Selenocysteine; Signal-anchor; Thyroid hormones biosynthesis;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..305
FT /note="Thyroxine 5-deiodinase"
FT /id="PRO_0000223868"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..63
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 64..305
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT ACT_SITE 171
FT NON_STD 171
FT /note="Selenocysteine"
FT CONFLICT 30
FT /note="R -> H (in Ref. 2; AAS48449)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 34042 MW; 119C9527D57302B2 CRC64;
MPGQAGRRRL VGGGCRGSQG PLGGAATMLR SLLLHSLRLC AQTASCLVLF PRFLGTACML
WLLDFLCIRK HLLGRRRRGE PETEVELNSD GDEVPPDDPP ICVSDDNRLC TLASLRAVWH
GQKLDFFKQA HEGGPAPNSE VVLPDGFQNQ HILDYARGNR PLVLNFGSCT UPPFMARMSA
FQRLVTKYQR DVDFLIIYIE EAHPSDGWVT TDSPYSIPQH RSLEDRVSAA RVLQQGAPEC
SLVLDTMANS SSSAYGAYFE RLYVIQSGTI MYQGGRGPDG YQVSELRTWL ERYDQQLHGP
QPRRV
