ID   IOJAP_ALKHC             Reviewed;         117 AA.
AC   Q9KD89;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Ribosomal silencing factor RsfS {ECO:0000255|HAMAP-Rule:MF_01477};
GN   Name=rsfS {ECO:0000255|HAMAP-Rule:MF_01477}; OrderedLocusNames=BH1328;
OS   Alkalihalobacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344
OS   / JCM 9153 / C-125) (Bacillus halodurans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalihalobacillus.
OX   NCBI_TaxID=272558;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125;
RX   PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA   Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N., Fuji F.,
RA   Hirama C., Nakamura Y., Ogasawara N., Kuhara S., Horikoshi K.;
RT   "Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans
RT   and genomic sequence comparison with Bacillus subtilis.";
RL   Nucleic Acids Res. 28:4317-4331(2000).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS).
RA   Benach J., Su M., Seetharaman J., Ho C.H., Janjua H., Cunningham K.,
RA   Ma L.-C., Xiao R., Liu J., Baran M.C., Acton T.B., Rost B.,
RA   Montelione G.T., Hunt J.F., Tong L.;
RT   "Crystal structure of Q9KD89 from Bacillus halodurans. Northeast structural
RT   genomics target BhR21.";
RL   Submitted (DEC-2006) to the PDB data bank.
CC   -!- FUNCTION: Functions as a ribosomal silencing factor. Interacts with
CC       ribosomal protein L14 (rplN), blocking formation of intersubunit bridge
CC       B8. Prevents association of the 30S and 50S ribosomal subunits and the
CC       formation of functional ribosomes, thus repressing translation.
CC       {ECO:0000255|HAMAP-Rule:MF_01477}.
CC   -!- SUBUNIT: Interacts with ribosomal protein L14 (rplN).
CC       {ECO:0000255|HAMAP-Rule:MF_01477}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01477}.
CC   -!- SIMILARITY: Belongs to the Iojap/RsfS family. {ECO:0000255|HAMAP-
CC       Rule:MF_01477}.
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DR   EMBL; BA000004; BAB05047.1; -; Genomic_DNA.
DR   PIR; H83815; H83815.
DR   RefSeq; WP_010897495.1; NC_002570.2.
DR   PDB; 2O5A; X-ray; 2.70 A; A/B=1-117.
DR   PDBsum; 2O5A; -.
DR   AlphaFoldDB; Q9KD89; -.
DR   SMR; Q9KD89; -.
DR   STRING; 272558.10173944; -.
DR   EnsemblBacteria; BAB05047; BAB05047; BAB05047.
DR   KEGG; bha:BH1328; -.
DR   eggNOG; COG0799; Bacteria.
DR   HOGENOM; CLU_092688_2_2_9; -.
DR   OMA; VVHVFQK; -.
DR   OrthoDB; 1990650at2; -.
DR   EvolutionaryTrace; Q9KD89; -.
DR   Proteomes; UP000001258; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042256; P:mature ribosome assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0090071; P:negative regulation of ribosome biogenesis; IEA:UniProtKB-UniRule.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.460.10; -; 1.
DR   HAMAP; MF_01477; Iojap_RsfS; 1.
DR   InterPro; IPR004394; Iojap/RsfS/C7orf30.
DR   InterPro; IPR043519; NT_sf.
DR   PANTHER; PTHR21043; PTHR21043; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
DR   TIGRFAMs; TIGR00090; rsfS_iojap_ybeB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Reference proteome; Repressor;
KW   Translation regulation.
FT   CHAIN           1..117
FT                   /note="Ribosomal silencing factor RsfS"
FT                   /id="PRO_0000419623"
FT   HELIX           4..16
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   STRAND          20..26
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   STRAND          36..44
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   HELIX           45..61
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   STRAND          68..71
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   TURN            72..75
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   STRAND          76..81
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   STRAND          83..91
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   HELIX           95..97
FT                   /evidence="ECO:0007829|PDB:2O5A"
FT   TURN            100..102
FT                   /evidence="ECO:0007829|PDB:2O5A"
SQ   SEQUENCE   117 AA;  13201 MW;  139776DB1DFE892F CRC64;
     MSNQELLQLA VNAVDDKKAE QVVALNMKGI SLIADFFLIC HGNSEKQVQA IAHELKKVAQ
     EQGIEIKRLE GYEQARWVLI DLGDVVVHVF HKDERAYYNL EKLWGDAPTV ELEGVIS