IOJAP_CHRVO
ID IOJAP_CHRVO Reviewed; 122 AA.
AC Q7P0P8;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Ribosomal silencing factor RsfS {ECO:0000255|HAMAP-Rule:MF_01477};
GN Name=rsfS {ECO:0000255|HAMAP-Rule:MF_01477}; OrderedLocusNames=CV_0518;
OS Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC
OS 12614 / NCIMB 9131 / NCTC 9757).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
OC Chromobacteriaceae; Chromobacterium.
OX NCBI_TaxID=243365;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC
RC 9757;
RX PubMed=14500782; DOI=10.1073/pnas.1832124100;
RA Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T.,
RA Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R.,
RA Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F.,
RA Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S.,
RA Belo A., van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M.,
RA Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P.,
RA Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S.,
RA Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N.,
RA Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P.,
RA Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R.,
RA Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B.,
RA Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J.,
RA Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F.,
RA Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z.,
RA de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F.,
RA Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A.,
RA Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C.,
RA Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M.,
RA Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C.,
RA Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R.,
RA Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M.,
RA Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R.,
RA Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G.;
RT "The complete genome sequence of Chromobacterium violaceum reveals
RT remarkable and exploitable bacterial adaptability.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RG Northeast structural genomics consortium (NESG);
RT "X-ray crystal structure of protein CV0518 from Chromobacterium
RT violaceum.";
RL Submitted (JUL-2011) to the PDB data bank.
CC -!- FUNCTION: Functions as a ribosomal silencing factor. Interacts with
CC ribosomal protein L14 (rplN), blocking formation of intersubunit bridge
CC B8. Prevents association of the 30S and 50S ribosomal subunits and the
CC formation of functional ribosomes, thus repressing translation.
CC {ECO:0000255|HAMAP-Rule:MF_01477}.
CC -!- SUBUNIT: Interacts with ribosomal protein L14 (rplN).
CC {ECO:0000255|HAMAP-Rule:MF_01477}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01477}.
CC -!- SIMILARITY: Belongs to the Iojap/RsfS family. {ECO:0000255|HAMAP-
CC Rule:MF_01477}.
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DR EMBL; AE016825; AAQ58195.1; -; Genomic_DNA.
DR RefSeq; WP_011134073.1; NC_005085.1.
DR PDB; 2ID1; X-ray; 3.00 A; A/B=1-122.
DR PDBsum; 2ID1; -.
DR AlphaFoldDB; Q7P0P8; -.
DR SMR; Q7P0P8; -.
DR STRING; 243365.CV_0518; -.
DR EnsemblBacteria; AAQ58195; AAQ58195; CV_0518.
DR GeneID; 66365572; -.
DR KEGG; cvi:CV_0518; -.
DR eggNOG; COG0799; Bacteria.
DR HOGENOM; CLU_092688_6_1_4; -.
DR OMA; VVHVFQK; -.
DR OrthoDB; 1990650at2; -.
DR EvolutionaryTrace; Q7P0P8; -.
DR Proteomes; UP000001424; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042256; P:mature ribosome assembly; IEA:UniProtKB-UniRule.
DR GO; GO:0090071; P:negative regulation of ribosome biogenesis; IEA:UniProtKB-UniRule.
DR GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.460.10; -; 1.
DR HAMAP; MF_01477; Iojap_RsfS; 1.
DR InterPro; IPR004394; Iojap/RsfS/C7orf30.
DR InterPro; IPR043519; NT_sf.
DR PANTHER; PTHR21043; PTHR21043; 1.
DR SUPFAM; SSF81301; SSF81301; 1.
DR TIGRFAMs; TIGR00090; rsfS_iojap_ybeB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; Repressor;
KW Translation regulation.
FT CHAIN 1..122
FT /note="Ribosomal silencing factor RsfS"
FT /id="PRO_0000419624"
FT HELIX 3..16
FT /evidence="ECO:0007829|PDB:2ID1"
FT STRAND 20..26
FT /evidence="ECO:0007829|PDB:2ID1"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:2ID1"
FT STRAND 35..41
FT /evidence="ECO:0007829|PDB:2ID1"
FT HELIX 45..61
FT /evidence="ECO:0007829|PDB:2ID1"
FT TURN 72..74
FT /evidence="ECO:0007829|PDB:2ID1"
FT STRAND 75..82
FT /evidence="ECO:0007829|PDB:2ID1"
FT STRAND 85..90
FT /evidence="ECO:0007829|PDB:2ID1"
FT HELIX 92..98
FT /evidence="ECO:0007829|PDB:2ID1"
FT HELIX 100..103
FT /evidence="ECO:0007829|PDB:2ID1"
SQ SEQUENCE 122 AA; 13193 MW; 80EDD9D5B220E909 CRC64;
MEIQEISKLA IEALEDIKGK DIIELDTSKL TSLFQRMIVA TGDSNRQVKA LANSVQVKLK
EAGVDIVGSE GHESGEWVLV DAGDVVVHVM LPAVRDYYDI EALWGGQKPS FAVGAAKPWS
AV
