ID   IOJAP_PSEAB             Reviewed;         118 AA.
AC   Q02SH2;
DT   07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT   14-NOV-2006, sequence version 1.
DT   03-AUG-2022, entry version 69.
DE   RecName: Full=Ribosomal silencing factor RsfS {ECO:0000255|HAMAP-Rule:MF_01477};
GN   Name=rsfS {ECO:0000255|HAMAP-Rule:MF_01477};
GN   OrderedLocusNames=PA14_12030 {ECO:0000312|EMBL:ABJ13279.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND PHOSPHORYLATION AT TYR-36.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=25096199; DOI=10.1007/s00216-014-8045-8;
RA   Ouidir T., Jarnier F., Cosette P., Jouenne T., Hardouin J.;
RT   "Potential of liquid-isoelectric-focusing protein fractionation to improve
RT   phosphoprotein characterization of Pseudomonas aeruginosa PA14.";
RL   Anal. Bioanal. Chem. 406:6297-6309(2014).
CC   -!- FUNCTION: Functions as a ribosomal silencing factor. Interacts with
CC       ribosomal protein L14 (rplN), blocking formation of intersubunit bridge
CC       B8. Prevents association of the 30S and 50S ribosomal subunits and the
CC       formation of functional ribosomes, thus repressing translation.
CC       {ECO:0000255|HAMAP-Rule:MF_01477}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01477}.
CC   -!- SIMILARITY: Belongs to the Iojap/RsfS family. {ECO:0000255|HAMAP-
CC       Rule:MF_01477}.
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DR   EMBL; CP000438; ABJ13279.1; -; Genomic_DNA.
DR   RefSeq; WP_003100305.1; NZ_CP034244.1.
DR   AlphaFoldDB; Q02SH2; -.
DR   SMR; Q02SH2; -.
DR   iPTMnet; Q02SH2; -.
DR   PRIDE; Q02SH2; -.
DR   EnsemblBacteria; ABJ13279; ABJ13279; PA14_12030.
DR   KEGG; pau:PA14_12030; -.
DR   HOGENOM; CLU_092688_6_1_6; -.
DR   OMA; VVHVFQK; -.
DR   BioCyc; PAER208963:G1G74-999-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042256; P:mature ribosome assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0090071; P:negative regulation of ribosome biogenesis; IEA:UniProtKB-UniRule.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.460.10; -; 1.
DR   HAMAP; MF_01477; Iojap_RsfS; 1.
DR   InterPro; IPR004394; Iojap/RsfS/C7orf30.
DR   InterPro; IPR043519; NT_sf.
DR   PANTHER; PTHR21043; PTHR21043; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
DR   TIGRFAMs; TIGR00090; rsfS_iojap_ybeB; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphoprotein; Repressor; Translation regulation.
FT   CHAIN           1..118
FT                   /note="Ribosomal silencing factor RsfS"
FT                   /id="PRO_0000431473"
FT   MOD_RES         36
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000269|PubMed:25096199"
SQ   SEQUENCE   118 AA;  13054 MW;  755E7999A9DF0201 CRC64;
     MQTEQLVQVA IDALEDLKAQ DIVTLDVRDK TSVTDYMVIA CGSSSRQVKS LADNVLTKAK
     ENGVKPLGSE GLESGEWALL DLGDVVVHVM LPATRQFYDL ERLWQGAEQS RAQHQPEE