ID   IOJAP_STRPN             Reviewed;         117 AA.
AC   Q97P97;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Ribosomal silencing factor RsfS;
GN   Name=rsfS; OrderedLocusNames=SP_1744;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
RN   [2]
RP   INTERACTION WITH RPLN.
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=22829778; DOI=10.1371/journal.pgen.1002815;
RA   Hauser R., Pech M., Kijek J., Yamamoto H., Titz B., Naeve F.,
RA   Tovchigrechko A., Yamamoto K., Szaflarski W., Takeuchi N., Stellberger T.,
RA   Diefenbacher M.E., Nierhaus K.H., Uetz P.;
RT   "RsfA (YbeB) proteins are conserved ribosomal silencing factors.";
RL   PLoS Genet. 8:E1002815-E1002815(2012).
CC   -!- FUNCTION: Functions as a ribosomal silencing factor. Interacts with
CC       ribosomal protein L14 (rplN), blocking formation of intersubunit bridge
CC       B8. Prevents association of the 30S and 50S ribosomal subunits and the
CC       formation of functional ribosomes, thus repressing translation (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with ribosomal protein L14 (rplN).
CC       {ECO:0000269|PubMed:22829778}.
CC   -!- INTERACTION:
CC       Q97P97; Q97NX8: SP_1873; NbExp=4; IntAct=EBI-6473708, EBI-6473712;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Iojap/RsfS family. {ECO:0000305}.
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DR   EMBL; AE005672; AAK75820.1; -; Genomic_DNA.
DR   PIR; C95203; C95203.
DR   RefSeq; WP_001003023.1; NZ_AKVY01000001.1.
DR   AlphaFoldDB; Q97P97; -.
DR   SMR; Q97P97; -.
DR   IntAct; Q97P97; 1.
DR   STRING; 170187.SP_1744; -.
DR   EnsemblBacteria; AAK75820; AAK75820; SP_1744.
DR   KEGG; spn:SP_1744; -.
DR   eggNOG; COG0799; Bacteria.
DR   OMA; VVHVFQK; -.
DR   PhylomeDB; Q97P97; -.
DR   BioCyc; SPNE170187:G1FZB-1767-MON; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0042256; P:mature ribosome assembly; IEA:UniProtKB-UniRule.
DR   GO; GO:0090071; P:negative regulation of ribosome biogenesis; IEA:UniProtKB-UniRule.
DR   GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.460.10; -; 1.
DR   HAMAP; MF_01477; Iojap_RsfS; 1.
DR   InterPro; IPR004394; Iojap/RsfS/C7orf30.
DR   InterPro; IPR043519; NT_sf.
DR   PANTHER; PTHR21043; PTHR21043; 1.
DR   SUPFAM; SSF81301; SSF81301; 1.
DR   TIGRFAMs; TIGR00090; rsfS_iojap_ybeB; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Repressor; Translation regulation.
FT   CHAIN           1..117
FT                   /note="Ribosomal silencing factor RsfS"
FT                   /id="PRO_0000419625"
SQ   SEQUENCE   117 AA;  12819 MW;  0B1AF8068A1AA499 CRC64;
     MNEKELLELV VKAADEKRAE DILALDVQDL TSVTDYLVIT SSMNSRQLDA IAANIREKVA
     QAGFKGSHVE GDAAGGWVLL DLGAVVVHIF SEEMRAHYNL EKLWHEANSV DISEALA