ID   IOLB_BACSU              Reviewed;         271 AA.
AC   P42413;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 2.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=5-deoxy-glucuronate isomerase;
DE            Short=5DG isomerase;
DE            EC=5.3.1.30;
GN   Name=iolB; Synonyms=yxdB; OrderedLocusNames=BSU39750; ORFNames=E83B;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / BGSC1A1;
RX   PubMed=7952181; DOI=10.1099/13500872-140-9-2289;
RA   Yoshida K., Sano H., Miwa Y., Ogasawara N., Fujita Y.;
RT   "Cloning and nucleotide sequencing of a 15 kb region of the Bacillus
RT   subtilis genome containing the iol operon.";
RL   Microbiology 140:2289-2298(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 82.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-71.
RC   STRAIN=168 / BGSC1A1;
RX   PubMed=7584049; DOI=10.1093/dnares/2.2.61;
RA   Yoshida K., Seki S., Fujimura M., Miwa Y., Fujita Y.;
RT   "Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome
RT   between the gnt and iol operons.";
RL   DNA Res. 2:61-69(1995).
RN   [5]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=168 / 60015;
RX   PubMed=18310071; DOI=10.1074/jbc.m708043200;
RA   Yoshida K., Yamaguchi M., Morinaga T., Kinehara M., Ikeuchi M., Ashida H.,
RA   Fujita Y.;
RT   "Myo-inositol catabolism in Bacillus subtilis.";
RL   J. Biol. Chem. 283:10415-10424(2008).
CC   -!- FUNCTION: Involved in the isomerization of 5-deoxy-glucuronate (5DG) to
CC       5-dehydro-2-deoxy-D-gluconate (DKG or 2-deoxy-5-keto-D-gluconate).
CC       {ECO:0000269|PubMed:18310071}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-deoxy-D-glucuronate = 5-dehydro-2-deoxy-D-gluconate;
CC         Xref=Rhea:RHEA:25840, ChEBI:CHEBI:16669, ChEBI:CHEBI:58852;
CC         EC=5.3.1.30; Evidence={ECO:0000269|PubMed:18310071};
CC   -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA;
CC       acetyl-CoA from myo-inositol: step 4/7.
CC   -!- SIMILARITY: Belongs to the isomerase IolB family. {ECO:0000305}.
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DR   EMBL; D14399; BAA03291.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB16011.2; -; Genomic_DNA.
DR   EMBL; AB005554; BAA21610.1; -; Genomic_DNA.
DR   PIR; B69645; B69645.
DR   RefSeq; NP_391854.2; NC_000964.3.
DR   RefSeq; WP_003243247.1; NZ_JNCM01000034.1.
DR   AlphaFoldDB; P42413; -.
DR   SMR; P42413; -.
DR   STRING; 224308.BSU39750; -.
DR   PaxDb; P42413; -.
DR   PRIDE; P42413; -.
DR   EnsemblBacteria; CAB16011; CAB16011; BSU_39750.
DR   GeneID; 937580; -.
DR   KEGG; bsu:BSU39750; -.
DR   PATRIC; fig|224308.179.peg.4300; -.
DR   eggNOG; COG3718; Bacteria.
DR   InParanoid; P42413; -.
DR   OMA; HGYDLYY; -.
DR   PhylomeDB; P42413; -.
DR   BioCyc; BSUB:BSU39750-MON; -.
DR   BioCyc; MetaCyc:BSU39750-MON; -.
DR   BRENDA; 5.3.1.30; 658.
DR   UniPathway; UPA00076; UER00920.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0102482; F:5-deoxy-D-glucuronate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008880; F:glucuronate isomerase activity; IEA:InterPro.
DR   GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.120.10; -; 2.
DR   HAMAP; MF_01673; IolB; 1.
DR   InterPro; IPR024203; Deoxy-glucuronate_isom_IolB.
DR   InterPro; IPR023770; IolB_Bacilli.
DR   InterPro; IPR021120; KduI/IolB_isomerase.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   PANTHER; PTHR39193; PTHR39193; 1.
DR   Pfam; PF04962; KduI; 1.
DR   PIRSF; PIRSF036628; IolB; 1.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   TIGRFAMs; TIGR04378; myo_inos_iolB; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Reference proteome.
FT   CHAIN           1..271
FT                   /note="5-deoxy-glucuronate isomerase"
FT                   /id="PRO_0000084212"
FT   CONFLICT        82
FT                   /note="V -> Y (in Ref. 1; BAA03291)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   271 AA;  30706 MW;  8CD4C4DC1149BEF5 CRC64;
     MSYLLRKPQS HEVSNGVKLV HEVTTSNSDL TYVEFKVLDL ASGSSYTEEL KKQEICIVAV
     TGKITVTDHE STFENIGTRE SVFERKPTDS VYISNDRAFE ITAVSDARVA LCYSPSEKQL
     PTKLIKAEDN GIEHRGQFSN KRTVHNILPD SDPSANSLLV VEVYTDSGNW SSYPPHKHDQ
     DNLPEESFLE ETYYHELDPG QGFVFQRVYT DDRSIDETMT VGNENVVIVP AGYHPVGVPD
     GYTSYYLNVM AGPTRKWKFY NDPAHEWILE R