4HYPE_AGRFC
ID 4HYPE_AGRFC Reviewed; 345 AA.
AC A9CKB4;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=4-hydroxyproline 2-epimerase {ECO:0000303|PubMed:24980702};
DE Short=4Hyp 2-epimerase;
DE Short=4HypE {ECO:0000303|PubMed:24980702};
DE EC=5.1.1.8 {ECO:0000269|PubMed:24980702};
GN OrderedLocusNames=Atu0398 {ECO:0000312|EMBL:AAK86213.1};
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND INDUCTION.
RC STRAIN=C58 / ATCC 33970;
RX PubMed=24980702; DOI=10.7554/elife.03275;
RA Zhao S., Sakai A., Zhang X., Vetting M.W., Kumar R., Hillerich B.,
RA San Francisco B., Solbiati J., Steves A., Brown S., Akiva E., Barber A.,
RA Seidel R.D., Babbitt P.C., Almo S.C., Gerlt J.A., Jacobson M.P.;
RT "Prediction and characterization of enzymatic activities guided by sequence
RT similarity and genome neighborhood networks.";
RL Elife 3:E03275-E03275(2014).
CC -!- FUNCTION: Catalyzes the epimerization of trans-4-hydroxy-L-proline
CC (t4LHyp) to cis-4-hydroxy-D-proline (c4DHyp). May be involved in a
CC degradation pathway of t4LHyp, which would allow A.tumefaciens to grow
CC on t4LHyp as a sole carbon source. Can also catalyze the epimerization
CC of trans-3-hydroxy-L-proline (t3LHyp) to cis-3-hydroxy-D-proline
CC (c3DHyp) in vitro. Displays no proline racemase activity.
CC {ECO:0000269|PubMed:24980702}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=trans-4-hydroxy-L-proline = cis-4-hydroxy-D-proline;
CC Xref=Rhea:RHEA:21152, ChEBI:CHEBI:57690, ChEBI:CHEBI:58375;
CC EC=5.1.1.8; Evidence={ECO:0000269|PubMed:24980702};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=4.6 mM for trans-4-hydroxy-L-proline
CC {ECO:0000269|PubMed:24980702};
CC KM=25 mM for trans-3-hydroxy-L-proline {ECO:0000269|PubMed:24980702};
CC Note=kcat is 0.86 sec(-1) for t4LHyp epimerization. kcat is 4.0 sec(-
CC 1) for t3LHyp epimerization. {ECO:0000269|PubMed:24980702};
CC -!- INDUCTION: Is up-regulated when the bacterium is grown on t4LHyp or
CC t3LHyp as sole carbon source. {ECO:0000269|PubMed:24980702}.
CC -!- SIMILARITY: Belongs to the proline racemase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE007869; AAK86213.1; -; Genomic_DNA.
DR PIR; AE2625; AE2625.
DR PIR; D97407; D97407.
DR RefSeq; NP_353428.1; NC_003062.2.
DR RefSeq; WP_010970869.1; NC_003062.2.
DR AlphaFoldDB; A9CKB4; -.
DR SMR; A9CKB4; -.
DR STRING; 176299.Atu0398; -.
DR EnsemblBacteria; AAK86213; AAK86213; Atu0398.
DR KEGG; atu:Atu0398; -.
DR PATRIC; fig|176299.10.peg.389; -.
DR eggNOG; COG3938; Bacteria.
DR HOGENOM; CLU_036729_2_0_5; -.
DR OMA; ERRAYCM; -.
DR PhylomeDB; A9CKB4; -.
DR BioCyc; AGRO:ATU0398-MON; -.
DR SABIO-RK; A9CKB4; -.
DR Proteomes; UP000000813; Chromosome circular.
DR GO; GO:0047580; F:4-hydroxyproline epimerase activity; IEA:UniProtKB-EC.
DR InterPro; IPR008794; Pro_racemase_fam.
DR PANTHER; PTHR33442; PTHR33442; 1.
DR Pfam; PF05544; Pro_racemase; 1.
DR PIRSF; PIRSF029792; Pro_racemase; 1.
DR SFLD; SFLDS00028; Proline_Racemase; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome.
FT CHAIN 1..345
FT /note="4-hydroxyproline 2-epimerase"
FT /id="PRO_0000432264"
FT ACT_SITE 93
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:B9JQV3"
FT ACT_SITE 255
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:B9JQV3"
FT BINDING 85
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B9JQV3"
FT BINDING 94..95
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B9JQV3"
FT BINDING 251
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B9JQV3"
FT BINDING 256..257
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:B9JQV3"
SQ SEQUENCE 345 AA; 36953 MW; 0484710115F9FE91 CRC64;
MRWKRTLQLL DVHCEGEIGR VVTGGAPKIP GNTVAEQLHW MNTDPQGEAL RRFLTLEPRG
TPMGSVDLLL PPKHPDAHAA FVILQPDQAH ASSGSNSICA TTALLESGMV EMQEPETVII
LETAAGLVKA TATCRDGRCE KVKLTMVPSF VHELDVSIDT PEWGRVTMDI SYGGIFYALV
DVRQIGLTIE KANAAKLVAA GMTLKDLVNR EMTVVHPEIP AISGVAYVMF RDVDADGSIR
TCTTMWPGRA DRSPCGTGNS ANLATLYARG KVKVGDEYKS RSIIGSEFDV GLSAVTEVAG
RPAVIPTIAG RGFTFGLHQV GLDPFDPLGD GFAMTDVWGP EAGNI
