IOLE_BACC3
ID IOLE_BACC3 Reviewed; 298 AA.
AC C1EVJ4;
DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Inosose dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE EC=4.2.1.44 {ECO:0000255|HAMAP-Rule:MF_01672};
DE AltName: Full=2-keto-myo-inositol dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE Short=2KMI dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
GN Name=iolE {ECO:0000255|HAMAP-Rule:MF_01672}; OrderedLocusNames=BCA_2600;
OS Bacillus cereus (strain 03BB102).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus;
OC Bacillus cereus group.
OX NCBI_TaxID=572264;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=03BB102;
RA Dodson R.J., Jackson P., Munk A.C., Brettin T., Bruce D., Detter C.,
RA Tapia R., Han C., Sutton G., Sims D.;
RT "Genome sequence of Bacillus cereus 03BB102.";
RL Submitted (FEB-2009) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the dehydration of inosose (2-keto-myo-inositol,
CC 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-
CC trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).
CC {ECO:0000255|HAMAP-Rule:MF_01672}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=scyllo-inosose = 3D-3,5/4-trihydroxycyclohexane-1,2-dione +
CC H2O; Xref=Rhea:RHEA:14065, ChEBI:CHEBI:15377, ChEBI:CHEBI:17811,
CC ChEBI:CHEBI:28446; EC=4.2.1.44; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01672};
CC -!- COFACTOR:
CC Name=glutathione; Xref=ChEBI:CHEBI:57925;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA;
CC acetyl-CoA from myo-inositol: step 2/7. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
CC -!- SIMILARITY: Belongs to the IolE/MocC family. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
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DR EMBL; CP001407; ACO30511.1; -; Genomic_DNA.
DR RefSeq; WP_000471988.1; NZ_CP009318.1.
DR AlphaFoldDB; C1EVJ4; -.
DR SMR; C1EVJ4; -.
DR EnsemblBacteria; ACO30511; ACO30511; BCA_2600.
DR KEGG; bcx:BCA_2600; -.
DR PATRIC; fig|572264.18.peg.2549; -.
DR OMA; VHCKDIR; -.
DR UniPathway; UPA00076; UER00144.
DR Proteomes; UP000002210; Chromosome.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050114; F:myo-inosose-2 dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01672; IolE; 1.
DR InterPro; IPR023952; IolE.
DR InterPro; IPR030823; IolE/MocC.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR04379; myo_inos_iolE; 1.
PE 3: Inferred from homology;
KW Cobalt; Lyase; Manganese.
FT CHAIN 1..298
FT /note="Inosose dehydratase"
FT /id="PRO_1000187324"
SQ SEQUENCE 298 AA; 33639 MW; 0236087B12403135 CRC64;
MFKENTIKLG IAPIAWTNDD MPELGAENTF EQCISEMALA GFNGSEVGNK YPRNTVVLKK
SLELRNLEIA SAWFSTFLTT KPLEETVEEF IKHRDFLHDM GAKVIVVSEQ GHSIQGLMDV
PLFKNKPVFT EEEWNKLADG LHHLGKLAQE KGLHIVYHHH MGTGVQTTAE IEKLMDITDS
ALVSLLFDTG HLVFSGEEPL YILKKYLPRI KHVHLKDIRQ EVVDIVKENE LSFLQAVKNG
AFTVPGDGVI EFDEVFTILA NSDYQGWFVV EAEQDPALAN PFEYALKARE FIREKAGL