IOLE_CLOBB
ID IOLE_CLOBB Reviewed; 298 AA.
AC B2TJ88;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Inosose dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE EC=4.2.1.44 {ECO:0000255|HAMAP-Rule:MF_01672};
DE AltName: Full=2-keto-myo-inositol dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE Short=2KMI dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
GN Name=iolE {ECO:0000255|HAMAP-Rule:MF_01672}; OrderedLocusNames=CLL_A1306;
OS Clostridium botulinum (strain Eklund 17B / Type B).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=935198;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Eklund 17B / Type B;
RA Brinkac L.M., Brown J.L., Bruce D., Detter C., Munk C., Smith L.A.,
RA Smith T.J., Sutton G., Brettin T.S.;
RT "Complete sequence of Clostridium botulinum strain Eklund.";
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the dehydration of inosose (2-keto-myo-inositol,
CC 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-
CC trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).
CC {ECO:0000255|HAMAP-Rule:MF_01672}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=scyllo-inosose = 3D-3,5/4-trihydroxycyclohexane-1,2-dione +
CC H2O; Xref=Rhea:RHEA:14065, ChEBI:CHEBI:15377, ChEBI:CHEBI:17811,
CC ChEBI:CHEBI:28446; EC=4.2.1.44; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01672};
CC -!- COFACTOR:
CC Name=glutathione; Xref=ChEBI:CHEBI:57925;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA;
CC acetyl-CoA from myo-inositol: step 2/7. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
CC -!- SIMILARITY: Belongs to the IolE/MocC family. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
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DR EMBL; CP001056; ACD24438.1; -; Genomic_DNA.
DR RefSeq; WP_012425191.1; NC_018648.1.
DR AlphaFoldDB; B2TJ88; -.
DR SMR; B2TJ88; -.
DR EnsemblBacteria; ACD24438; ACD24438; CLL_A1306.
DR KEGG; cbk:CLL_A1306; -.
DR PATRIC; fig|935198.13.peg.1252; -.
DR HOGENOM; CLU_059523_0_0_9; -.
DR OMA; VHCKDIR; -.
DR OrthoDB; 1194699at2; -.
DR UniPathway; UPA00076; UER00144.
DR Proteomes; UP000001195; Chromosome.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050114; F:myo-inosose-2 dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01672; IolE; 1.
DR InterPro; IPR023952; IolE.
DR InterPro; IPR030823; IolE/MocC.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR04379; myo_inos_iolE; 1.
PE 3: Inferred from homology;
KW Cobalt; Lyase; Manganese.
FT CHAIN 1..298
FT /note="Inosose dehydratase"
FT /id="PRO_0000352363"
SQ SEQUENCE 298 AA; 33865 MW; 2EE623365131E5BA CRC64;
MFNNDKVKIG ICPIGWTNDD MPDLGKENTF EQAVSEMALA GFKGTEIGNK YPKDVKVLKR
ALEMRNLQIA SAWFSSFLTT KPYEETEKEF IAHRDFLHAM GSKVIVVSEQ GHSIQGQMET
PIFDGKHHFN EEEWNLLAHG LNKLGQLAAD KEMRIVYHHH MGTGVQTTEE IDKLMSVTDE
NLVYLLFDTG HLVYSGENPV EILKKYVQRI KHVHLKDIRP EIVSKVKNEK LSFLKGVRAG
AFTVPGDGSI DFEPIFKILA ENNYEGWLMI EAEQDPSIAN PLEYAIKGRQ YIKEKASI