IOLE_CLOPE
ID IOLE_CLOPE Reviewed; 297 AA.
AC Q8XP74;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 90.
DE RecName: Full=Inosose dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE EC=4.2.1.44 {ECO:0000255|HAMAP-Rule:MF_01672};
DE AltName: Full=2-keto-myo-inositol dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE Short=2KMI dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
GN Name=iolE {ECO:0000255|HAMAP-Rule:MF_01672}; OrderedLocusNames=CPE0091;
OS Clostridium perfringens (strain 13 / Type A).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=195102;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=13 / Type A;
RX PubMed=11792842; DOI=10.1073/pnas.022493799;
RA Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T.,
RA Ogasawara N., Hattori M., Kuhara S., Hayashi H.;
RT "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-
RT eater.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002).
CC -!- FUNCTION: Catalyzes the dehydration of inosose (2-keto-myo-inositol,
CC 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-
CC trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).
CC {ECO:0000255|HAMAP-Rule:MF_01672}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=scyllo-inosose = 3D-3,5/4-trihydroxycyclohexane-1,2-dione +
CC H2O; Xref=Rhea:RHEA:14065, ChEBI:CHEBI:15377, ChEBI:CHEBI:17811,
CC ChEBI:CHEBI:28446; EC=4.2.1.44; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01672};
CC -!- COFACTOR:
CC Name=glutathione; Xref=ChEBI:CHEBI:57925;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA;
CC acetyl-CoA from myo-inositol: step 2/7. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
CC -!- SIMILARITY: Belongs to the IolE/MocC family. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
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DR EMBL; BA000016; BAB79797.1; -; Genomic_DNA.
DR RefSeq; WP_003470045.1; NC_003366.1.
DR AlphaFoldDB; Q8XP74; -.
DR SMR; Q8XP74; -.
DR STRING; 195102.gene:10489335; -.
DR EnsemblBacteria; BAB79797; BAB79797; BAB79797.
DR KEGG; cpe:CPE0091; -.
DR HOGENOM; CLU_059523_0_0_9; -.
DR OMA; VHCKDIR; -.
DR UniPathway; UPA00076; UER00144.
DR Proteomes; UP000000818; Chromosome.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050114; F:myo-inosose-2 dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01672; IolE; 1.
DR InterPro; IPR023952; IolE.
DR InterPro; IPR030823; IolE/MocC.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR04379; myo_inos_iolE; 1.
PE 3: Inferred from homology;
KW Cobalt; Lyase; Manganese; Reference proteome.
FT CHAIN 1..297
FT /note="Inosose dehydratase"
FT /id="PRO_0000352364"
SQ SEQUENCE 297 AA; 33753 MW; A43253E0917A105F CRC64;
MFNSNVKLGI APIAWTNDDM PDLGKENTFE QCISEMALAG FKGSEVGNKY PRDVEVLKKA
LELRDMEIAS AWFSAFLTTK PYEETEKAFI EHRDFLNAMG AKVIVVSEQG HSIQGQMETP
IFDGKYVLNE EEWKTLAEGL NKLGALAKEK GMKLVYHHHM GTVVQTTEEI DKLMDLTDEN
LVYLLFDSGH LVYSGEDALE VLKKYVNRVK HVHLKDIRKK KVEEVKRDKL SFLQGVRKGA
FTVPGDGDID FEPIFKVLDD NNYEGYLLVE AEQDPAIANP LEYAIKARKY IKEKTNL
