IOLE_GEOKA
ID IOLE_GEOKA Reviewed; 308 AA.
AC Q5KYR1;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Inosose dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE EC=4.2.1.44 {ECO:0000255|HAMAP-Rule:MF_01672};
DE AltName: Full=2-keto-myo-inositol dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE Short=2KMI dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
GN Name=iolE {ECO:0000255|HAMAP-Rule:MF_01672}; OrderedLocusNames=GK1890;
OS Geobacillus kaustophilus (strain HTA426).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus;
OC Geobacillus thermoleovorans group.
OX NCBI_TaxID=235909;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HTA426;
RX PubMed=15576355; DOI=10.1093/nar/gkh970;
RA Takami H., Takaki Y., Chee G.-J., Nishi S., Shimamura S., Suzuki H.,
RA Matsui S., Uchiyama I.;
RT "Thermoadaptation trait revealed by the genome sequence of thermophilic
RT Geobacillus kaustophilus.";
RL Nucleic Acids Res. 32:6292-6303(2004).
CC -!- FUNCTION: Catalyzes the dehydration of inosose (2-keto-myo-inositol,
CC 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-
CC trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).
CC {ECO:0000255|HAMAP-Rule:MF_01672}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=scyllo-inosose = 3D-3,5/4-trihydroxycyclohexane-1,2-dione +
CC H2O; Xref=Rhea:RHEA:14065, ChEBI:CHEBI:15377, ChEBI:CHEBI:17811,
CC ChEBI:CHEBI:28446; EC=4.2.1.44; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01672};
CC -!- COFACTOR:
CC Name=glutathione; Xref=ChEBI:CHEBI:57925;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA;
CC acetyl-CoA from myo-inositol: step 2/7. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
CC -!- SIMILARITY: Belongs to the IolE/MocC family. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
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DR EMBL; BA000043; BAD76175.1; -; Genomic_DNA.
DR RefSeq; WP_011231380.1; NC_006510.1.
DR AlphaFoldDB; Q5KYR1; -.
DR SMR; Q5KYR1; -.
DR STRING; 235909.GK1890; -.
DR EnsemblBacteria; BAD76175; BAD76175; GK1890.
DR KEGG; gka:GK1890; -.
DR eggNOG; COG1082; Bacteria.
DR HOGENOM; CLU_059523_0_0_9; -.
DR OMA; VHCKDIR; -.
DR UniPathway; UPA00076; UER00144.
DR Proteomes; UP000001172; Chromosome.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050114; F:myo-inosose-2 dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01672; IolE; 1.
DR InterPro; IPR023952; IolE.
DR InterPro; IPR030823; IolE/MocC.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR04379; myo_inos_iolE; 1.
PE 3: Inferred from homology;
KW Cobalt; Lyase; Manganese; Reference proteome.
FT CHAIN 1..308
FT /note="Inosose dehydratase"
FT /id="PRO_0000352367"
SQ SEQUENCE 308 AA; 35448 MW; 41D4CE64C2968AB9 CRC64;
MEKTVNPFRI GIAPISWVND DIPGLGDHYT QDQVLSEMAE LGYVATEMGR LFSQDPPSLR
AKLNQYGIEL ASKFIGVLFS DRSRLEEELK TFRSWAEYLH DMGCKYAIVC EMGGSMHWDP
RRAPEEKTIQ RLTDSEWESL VDGLHRAAHI CQELGMKLVY HFHAGTVVET AEEIDRLMEL
TDPNLVHLLY DTGHALYGGY DPVELLHRYA DRIQYVHLKD VRHDVLELVR REQLDFRTAV
LRGMFTVPGD GCIDFVPIFA KLIEMDYNGW IIVEAEQDPA VAHPYTYAKM AKEYIDRLVH
HLLAAQKR
