IOLE_KLEP7
ID IOLE_KLEP7 Reviewed; 299 AA.
AC A6THJ4;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Inosose dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE EC=4.2.1.44 {ECO:0000255|HAMAP-Rule:MF_01672};
DE AltName: Full=2-keto-myo-inositol dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
DE Short=2KMI dehydratase {ECO:0000255|HAMAP-Rule:MF_01672};
GN Name=iolE {ECO:0000255|HAMAP-Rule:MF_01672};
GN OrderedLocusNames=KPN78578_46040; ORFNames=KPN_04677;
OS Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=272620;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700721 / MGH 78578;
RG The Klebsiella pneumonia Genome Sequencing Project;
RA McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the dehydration of inosose (2-keto-myo-inositol,
CC 2KMI or 2,4,6/3,5-pentahydroxycyclohexanone) to 3D-(3,5/4)-
CC trihydroxycyclohexane-1,2-dione (D-2,3-diketo-4-deoxy-epi-inositol).
CC {ECO:0000255|HAMAP-Rule:MF_01672}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=scyllo-inosose = 3D-3,5/4-trihydroxycyclohexane-1,2-dione +
CC H2O; Xref=Rhea:RHEA:14065, ChEBI:CHEBI:15377, ChEBI:CHEBI:17811,
CC ChEBI:CHEBI:28446; EC=4.2.1.44; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01672};
CC -!- COFACTOR:
CC Name=glutathione; Xref=ChEBI:CHEBI:57925;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- COFACTOR:
CC Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01672};
CC -!- SIMILARITY: Belongs to the IolE/MocC family. {ECO:0000255|HAMAP-
CC Rule:MF_01672}.
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DR EMBL; CP000647; ABR80028.1; -; Genomic_DNA.
DR RefSeq; WP_002886970.1; NC_009648.1.
DR AlphaFoldDB; A6THJ4; -.
DR SMR; A6THJ4; -.
DR STRING; 272620.KPN_04677; -.
DR jPOST; A6THJ4; -.
DR PRIDE; A6THJ4; -.
DR EnsemblBacteria; ABR80028; ABR80028; KPN_04677.
DR GeneID; 64297355; -.
DR KEGG; kpn:KPN_04677; -.
DR HOGENOM; CLU_059523_0_0_6; -.
DR OMA; YDSRPEL; -.
DR Proteomes; UP000000265; Chromosome.
DR GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050114; F:myo-inosose-2 dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019310; P:inositol catabolic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01672; IolE; 1.
DR InterPro; IPR023952; IolE.
DR InterPro; IPR030823; IolE/MocC.
DR InterPro; IPR036237; Xyl_isomerase-like_sf.
DR InterPro; IPR013022; Xyl_isomerase-like_TIM-brl.
DR Pfam; PF01261; AP_endonuc_2; 1.
DR SUPFAM; SSF51658; SSF51658; 1.
DR TIGRFAMs; TIGR04379; myo_inos_iolE; 1.
PE 3: Inferred from homology;
KW Cobalt; Lyase; Manganese; Reference proteome.
FT CHAIN 1..299
FT /note="Inosose dehydratase"
FT /id="PRO_0000352371"
SQ SEQUENCE 299 AA; 33316 MW; F99B30E2FB28670E CRC64;
MNKDNVKLAI APIGWTNDDM PELGSENTFQ QIVSEMALAG FTGSEVGSKY PRDPAVLKPM
LDIRGIQICN AWFSTFFANG QREKTIDEFV NHMNFLHAMG AKVIGCSEQS GSIQGLDKPI
LGDAKPCFSD EEWQRVAEGY NTLGRLAAEK GMQVCLHHHM GTGIQTTAEI DKFMSLVDER
VFLLFDTGHA WYSEGGEAPM LAILKKYLPR INHVHLKDVR PPVIDQVRRD GLSFLDGVKK
GTFTVPGDGV IDFRPVFKLL DDFGYKGWMV VEAEQDPALA NPFEYAVKAR KYIRETAGI
