IOLF_BACSU
ID IOLF_BACSU Reviewed; 438 AA.
AC P42417;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Minor myo-inositol transporter IolF;
GN Name=iolF; Synonyms=yxdF; OrderedLocusNames=BSU39710; ORFNames=E83F;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BGSC1A1;
RX PubMed=7952181; DOI=10.1099/13500872-140-9-2289;
RA Yoshida K., Sano H., Miwa Y., Ogasawara N., Fujita Y.;
RT "Cloning and nucleotide sequencing of a 15 kb region of the Bacillus
RT subtilis genome containing the iol operon.";
RL Microbiology 140:2289-2298(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 32 AND 352-363.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 273-438.
RC STRAIN=168 / 60015;
RX PubMed=1761221; DOI=10.1016/0378-1119(91)90496-x;
RA Fujita Y., Shindo K., Miwa Y., Yoshida K.;
RT "Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and
RT expression in Escherichia coli.";
RL Gene 108:121-125(1991).
RN [5]
RP FUNCTION.
RX PubMed=11807058; DOI=10.1128/jb.184.4.983-991.2002;
RA Yoshida K., Yamamoto Y., Omae K., Yamamoto M., Fujita Y.;
RT "Identification of two myo-inositol transporter genes of Bacillus
RT subtilis.";
RL J. Bacteriol. 184:983-991(2002).
CC -!- FUNCTION: Minor myo-inositol uptake transporter.
CC {ECO:0000269|PubMed:11807058}.
CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- MISCELLANEOUS: The growth-defect caused by the iolF inactivation was
CC only observed in the iolT-null background.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; D14399; BAA03295.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB16007.2; -; Genomic_DNA.
DR EMBL; M76431; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; F69645; F69645.
DR RefSeq; NP_391850.2; NC_000964.3.
DR RefSeq; WP_003244597.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P42417; -.
DR SMR; P42417; -.
DR STRING; 224308.BSU39710; -.
DR TCDB; 2.A.1.1.27; the major facilitator superfamily (mfs).
DR PRIDE; P42417; -.
DR EnsemblBacteria; CAB16007; CAB16007; BSU_39710.
DR GeneID; 937616; -.
DR KEGG; bsu:BSU39710; -.
DR PATRIC; fig|224308.179.peg.4296; -.
DR eggNOG; COG2814; Bacteria.
DR InParanoid; P42417; -.
DR OMA; ANYIDAG; -.
DR PhylomeDB; P42417; -.
DR BioCyc; BSUB:BSU39710-MON; -.
DR UniPathway; UPA00076; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..438
FT /note="Minor myo-inositol transporter IolF"
FT /id="PRO_0000050304"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..334
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 352..363
FT /note="YRASAQGLMFFL -> LIRCFRTRTDVFP (in Ref. 1; BAA03295
FT and 3; M76431)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 438 AA; 46864 MW; 11D2DDD4501DC9FF CRC64;
MGNTNGDSAF NKRTIAAALA NYIDAGSIVA GSAGLSLWVS YLKLSDTQIG LLGALSANAI
SAAVGALLGG FLADKVGRKA VYTNSMLVYA LGICLVLFGV NFPMLLSGYI IIGLSVGADI
TASWTIIAEN APKKNRARHC GVAQVAWAAG AVVVLLLSVL AGDLGLLGNK IVFAHLLVIA
LITYILRIRL PESDAWQTKN QPEEAQAEKP AVLNKTSYFD LLKPMYLKSI LFLMGVYLVW
NLAAGVMGFF MPYIYQQVGG VSANMANLLQ MGLFIFTGLG VALIFMPFAD KYRKTVFGIA
AFMAVIGWTL FLLPVEGLPI LLLFIVVIGI NNGAGQQANY QLWASEIFPT QYRASAQGLM
FFLVRISIGI WSLFVPMIIT NFGIGTMAAI LLGCVTASMI IGLLFAPNTS GKSLEQIQEE
LYGSPQSQVK KGTESKIM
