IOLT_BACSU
ID IOLT_BACSU Reviewed; 473 AA.
AC O34718; Q797C3;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Major myo-inositol transporter IolT;
GN Name=iolT; Synonyms=ydjK; OrderedLocusNames=BSU06230;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=9455482; DOI=10.1093/dnares/4.5.335;
RA Kasahara Y., Nakai S., Ogasawara N., Yata K., Sadaie Y.;
RT "Sequence analysis of the groESL-cotA region of the Bacillus subtilis
RT genome, containing the restriction/modification system genes.";
RL DNA Res. 4:335-339(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION.
RX PubMed=11807058; DOI=10.1128/jb.184.4.983-991.2002;
RA Yoshida K., Yamamoto Y., Omae K., Yamamoto M., Fujita Y.;
RT "Identification of two myo-inositol transporter genes of Bacillus
RT subtilis.";
RL J. Bacteriol. 184:983-991(2002).
CC -!- FUNCTION: Major myo-inositol uptake transporter.
CC {ECO:0000269|PubMed:11807058}.
CC -!- PATHWAY: Polyol metabolism; myo-inositol degradation into acetyl-CoA.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Negatively regulated by IolR. Induced by inositol.
CC -!- MISCELLANEOUS: When iolT is inactivated, inositol uptake is almost
CC abolished even though iolF is active.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sugar
CC transporter (TC 2.A.1.1) family. {ECO:0000305}.
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DR EMBL; AB007638; BAA22766.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12442.1; -; Genomic_DNA.
DR PIR; G69789; G69789.
DR RefSeq; NP_388504.1; NC_000964.3.
DR RefSeq; WP_003234027.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; O34718; -.
DR SMR; O34718; -.
DR STRING; 224308.BSU06230; -.
DR TCDB; 2.A.1.1.26; the major facilitator superfamily (mfs).
DR PaxDb; O34718; -.
DR EnsemblBacteria; CAB12442; CAB12442; BSU_06230.
DR GeneID; 936014; -.
DR KEGG; bsu:BSU06230; -.
DR PATRIC; fig|224308.179.peg.675; -.
DR eggNOG; COG2814; Bacteria.
DR InParanoid; O34718; -.
DR OMA; SCHEQEM; -.
DR PhylomeDB; O34718; -.
DR BioCyc; BSUB:BSU06230-MON; -.
DR UniPathway; UPA00076; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR003663; Sugar/inositol_transpt.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR PRINTS; PR00171; SUGRTRNSPORT.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00879; SP; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..473
FT /note="Major myo-inositol transporter IolT"
FT /id="PRO_0000050305"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 111..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..166
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 350..370
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 473 AA; 51633 MW; 5A44A4AA09A227FF CRC64;
MNKQGNQMSF LRTIILVSTF GGLLFGYDTG VLNGALPYMG EPDQLNLNAF TEGLVTSSLL
FGAALGAVFG GRMSDFNGRR KNILFLAVIF FISTIGCTFA PNVTVMIISR FVLGIAVGGA
SVTVPAYLAE MSPVESRGRM VTQNELMIVS GQLLAFVFNA ILGTTMGDNS HVWRFMLVIA
SLPALFLFFG MIRMPESPRW LVSKGRKEDA LRVLKKIRDE KRAAAELQEI EFAFKKEDQL
EKATFKDLSV PWVRRIVFIG LGIAIVQQIT GVNSIMYYGT EILRNSGFQT EAALIGNIAN
GVISVLATFV GIWLLGRVGR RPMLMTGLIG TTTALLLIGI FSLVLEGSPA LPYVVLSLTV
TFLAFQQGAI SPVTWLMLSE IFPLRLRGLG MGVTVFCLWM VNFAVSFTFP ILLAAIGLST
TFFIFVGLGI CSVLFVKRFL PETKGLSLEQ LEENFRAYDH SGAKKDSGAE VIG
