ID   IORF_CVMA5              Reviewed;         207 AA.
AC   P69614; P18453; P18456;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   29-SEP-2021, entry version 49.
DE   RecName: Full=Protein I;
DE   AltName: Full=Accessory protein N2;
DE   AltName: Full=N internal ORF protein;
DE            Short=IORF;
DE   AltName: Full=Protein in nucleocapsid ORF;
GN   Name=N; Synonyms=I; ORFNames=7b;
OS   Murine coronavirus (strain A59) (MHV-A59) (Murine hepatitis virus).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Betacoronavirus; Embecovirus.
OX   NCBI_TaxID=11142;
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6687635; DOI=10.1093/nar/11.3.883;
RA   Armstrong J., Smeekens S., Rottier P.J.M.;
RT   "Sequence of the nucleocapsid gene from murine coronavirus MHV-A59.";
RL   Nucleic Acids Res. 11:883-891(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=2171216; DOI=10.1016/0042-6822(90)90316-j;
RA   Parker M.M., Masters P.S.;
RT   "Sequence comparison of the N genes of five strains of the coronavirus
RT   mouse hepatitis virus suggests a three domain structure for the
RT   nucleocapsid protein.";
RL   Virology 179:463-468(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate C12 mutant;
RX   PubMed=9426441; DOI=10.1006/viro.1997.8877;
RA   Leparc-Goffart I., Hingley S.T., Chua M.M., Jiang X., Lavi E., Weiss S.R.;
RT   "Altered pathogenesis of a mutant of the murine coronavirus MHV-A59 is
RT   associated with a Q159L amino acid substitution in the spike protein.";
RL   Virology 239:1-10(1997).
RN   [4]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=8995618; DOI=10.1128/jvi.71.2.996-1003.1997;
RA   Fischer F., Peng D., Hingley S.T., Weiss S.R., Masters P.S.;
RT   "The internal open reading frame within the nucleocapsid gene of mouse
RT   hepatitis virus encodes a structural protein that is not essential for
RT   viral replication.";
RL   J. Virol. 71:996-1003(1997).
CC   -!- FUNCTION: Structural protein that is not essential for the viral
CC       replication either in tissue culture or in its natural host. Confers a
CC       small growth advantage. {ECO:0000269|PubMed:8995618}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305|PubMed:8995618}.
CC   -!- MISCELLANEOUS: The gene encoding this protein is included within the N
CC       gene (alternative ORF).
CC   -!- SIMILARITY: Belongs to the coronavirus I protein family. {ECO:0000305}.
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DR   EMBL; X00509; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; M35256; AAA46448.1; -; Genomic_RNA.
DR   EMBL; AF029248; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   PIR; E45340; E45340.
DR   Proteomes; UP000007192; Genome.
DR   CDD; cd21662; embe-CoV_Protein-I_like; 1.
DR   InterPro; IPR004876; Corona_nucI.
DR   InterPro; IPR044311; N2-like_embe-CoV.
DR   Pfam; PF03187; Corona_I; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Virion.
FT   CHAIN           1..207
FT                   /note="Protein I"
FT                   /id="PRO_0000106122"
FT   CONFLICT        126
FT                   /note="V -> E (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   207 AA;  22587 MW;  70C342F25FA97B02 CRC64;
     MESSRRPLGL TKPSVDQIIK IEAEGISQSR LQLLNPTPGV WFPITPGFLA LPSSKRERSF
     SLQKDKECLL PMESPLQSKR DIGIDTTAVL LKHLMGSRSN YCPDGIFTIL AQGPMLEPVM
     ETALKVSSGL QTAKRTPIPA LILSKGTQAV MRLFLLGLRP ARYCLRAFML KALEGLHLLA
     DLVRGHNPVG QIIALEAVPT SASLPLL