ID   IP21_TOBAC              Reviewed;         197 AA.
AC   Q40561;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Proteinase inhibitor type-2;
DE   AltName: Full=Proteinase inhibitor type II;
DE   Flags: Precursor;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Samsun NN; TISSUE=Leaf;
RX   PubMed=7766901; DOI=10.1007/bf00020893;
RA   Balandin M.T., van der Does C., Albert J.M., Bol J.F., Linthorst H.J.M.;
RT   "Structure and induction pattern of a novel proteinase inhibitor class II
RT   gene of tobacco.";
RL   Plant Mol. Biol. 27:1197-1204(1995).
CC   -!- INDUCTION: Locally induced in leaves subjected to different types of
CC       stress (TMV infection, wounding, UV irradiation).
CC   -!- SIMILARITY: Belongs to the protease inhibitor I20 (potato type II
CC       proteinase inhibitor) family. {ECO:0000305}.
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DR   EMBL; Z29537; CAA82652.1; -; Genomic_DNA.
DR   PIR; S56662; S56662.
DR   RefSeq; XP_016497298.1; XM_016641812.1.
DR   AlphaFoldDB; Q40561; -.
DR   SMR; Q40561; -.
DR   STRING; 4097.Q40561; -.
DR   MEROPS; I20.002; -.
DR   MEROPS; I20.951; -.
DR   MEROPS; I20.952; -.
DR   GeneID; 107816122; -.
DR   KEGG; nta:107816122; -.
DR   OMA; QECINCC; -.
DR   OrthoDB; 1220099at2759; -.
DR   PhylomeDB; Q40561; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR003465; Prot_inh_I20.
DR   Pfam; PF02428; Prot_inhib_II; 3.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Repeat;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..197
FT                   /note="Proteinase inhibitor type-2"
FT                   /id="PRO_0000025320"
FT   REPEAT          24..80
FT                   /note="1"
FT   REPEAT          81..140
FT                   /note="2"
FT   REPEAT          141..196
FT                   /note="3"
FT   SITE            29..30
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000255"
FT   DISULFID        27..115
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..111
FT                   /evidence="ECO:0000250"
FT   DISULFID        39..121
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..88
FT                   /evidence="ECO:0000250"
FT   DISULFID        54..72
FT                   /evidence="ECO:0000250"
FT   DISULFID        55..84
FT                   /evidence="ECO:0000250"
FT   DISULFID        61..97
FT                   /evidence="ECO:0000250"
FT   DISULFID        114..132
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   197 AA;  20985 MW;  F9CD5CB4267A9710 CRC64;
     MAVHKVSFVA HLLVLGMFLL LVDAKACTKE CGNFAYGICP RSQGTPDDPI CTTCCAGYKG
     CNYYSANGTF ICEGSSDPKN PNVCPQFCDP DIAYSKCPRS EGETIINPTG CTTCCTGYKG
     CYYFGQDGEF VCEGESDEPK SCTTECDPRV ATISCPFSGL VKINQECINC CNADKGCELY
     DNDGSLICTG GEPQSAA