IP6K3_MOUSE
ID IP6K3_MOUSE Reviewed; 396 AA.
AC Q8BWD2;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Inositol hexakisphosphate kinase 3;
DE Short=InsP6 kinase 3;
DE EC=2.7.4.21;
DE AltName: Full=Inositol hexaphosphate kinase 3;
GN Name=Ip6k3; Synonyms=Ihpk3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP TISSUE SPECIFICITY.
RX PubMed=11502751; DOI=10.1074/jbc.m106842200;
RA Saiardi A., Nagata E., Luo H.R., Snowman A.M., Snyder S.H.;
RT "Identification and characterization of a novel inositol hexakisphosphate
RT kinase.";
RL J. Biol. Chem. 276:39179-39185(2001).
CC -!- FUNCTION: Converts inositol hexakisphosphate (InsP6) to
CC diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts
CC 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1D-myo-inositol hexakisphosphate + ATP = 5-diphospho-1D-myo-
CC inositol 1,2,3,4,6-pentakisphosphate + ADP; Xref=Rhea:RHEA:12793,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:58130, ChEBI:CHEBI:58628,
CC ChEBI:CHEBI:456216; EC=2.7.4.21;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-diphospho-myo-inositol 2,3,4,5,6-pentakisphosphate + ATP +
CC H(+) = 1,5-bis(diphospho)-1D-myo-inositol 2,3,4,6-tetrakisphosphate +
CC ADP; Xref=Rhea:RHEA:37467, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:74946, ChEBI:CHEBI:77983, ChEBI:CHEBI:456216;
CC EC=2.7.4.21;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Highly expressed in cerebellum, brain cortex,
CC kidney, thymus and lung. Detected at lower levels in hippocampus,
CC testis, heart and olfactory bulb. {ECO:0000269|PubMed:11502751}.
CC -!- SIMILARITY: Belongs to the inositol phosphokinase (IPK) family.
CC {ECO:0000305}.
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DR EMBL; AK052857; BAC35176.1; -; mRNA.
DR CCDS; CCDS28562.1; -.
DR RefSeq; NP_766615.1; NM_173027.2.
DR AlphaFoldDB; Q8BWD2; -.
DR SMR; Q8BWD2; -.
DR STRING; 10090.ENSMUSP00000025046; -.
DR PhosphoSitePlus; Q8BWD2; -.
DR PaxDb; Q8BWD2; -.
DR PRIDE; Q8BWD2; -.
DR Antibodypedia; 45687; 146 antibodies from 29 providers.
DR DNASU; 271424; -.
DR Ensembl; ENSMUST00000025046; ENSMUSP00000025046; ENSMUSG00000024210.
DR GeneID; 271424; -.
DR KEGG; mmu:271424; -.
DR UCSC; uc008bfl.1; mouse.
DR CTD; 117283; -.
DR MGI; MGI:3045325; Ip6k3.
DR VEuPathDB; HostDB:ENSMUSG00000024210; -.
DR eggNOG; KOG1620; Eukaryota.
DR GeneTree; ENSGT00940000160887; -.
DR HOGENOM; CLU_014862_0_0_1; -.
DR InParanoid; Q8BWD2; -.
DR OMA; YDGPDHG; -.
DR OrthoDB; 1102768at2759; -.
DR PhylomeDB; Q8BWD2; -.
DR TreeFam; TF314066; -.
DR BRENDA; 2.7.4.21; 3474.
DR Reactome; R-MMU-1855167; Synthesis of pyrophosphates in the cytosol.
DR BioGRID-ORCS; 271424; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q8BWD2; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q8BWD2; protein.
DR Bgee; ENSMUSG00000024210; Expressed in hindlimb stylopod muscle and 32 other tissues.
DR Genevisible; Q8BWD2; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0052723; F:inositol hexakisphosphate 1-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0052724; F:inositol hexakisphosphate 3-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0000832; F:inositol hexakisphosphate 5-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0000831; F:inositol hexakisphosphate 6-kinase activity; ISO:MGI.
DR GO; GO:0000828; F:inositol hexakisphosphate kinase activity; ISO:MGI.
DR GO; GO:0000827; F:inositol-1,3,4,5,6-pentakisphosphate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016301; F:kinase activity; IBA:GO_Central.
DR GO; GO:0032958; P:inositol phosphate biosynthetic process; ISO:MGI.
DR GO; GO:0040011; P:locomotion; IMP:MGI.
DR GO; GO:0046488; P:phosphatidylinositol metabolic process; ISO:MGI.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0006468; P:protein phosphorylation; ISO:MGI.
DR Gene3D; 3.30.470.160; -; 1.
DR InterPro; IPR005522; IPK.
DR InterPro; IPR038286; IPK_sf.
DR PANTHER; PTHR12400; PTHR12400; 1.
DR Pfam; PF03770; IPK; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Reference proteome;
KW Transferase.
FT CHAIN 1..396
FT /note="Inositol hexakisphosphate kinase 3"
FT /id="PRO_0000066882"
FT BINDING 206..214
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 396 AA; 44418 MW; DB98CB79B4B36EE0 CRC64;
MVVRHSSDKG KIGVGVPLEP FLHQVGGHLS VLQYDAYTVC KPLVSQEQKF YESLPLAMKC
FTPKYKGTIT VRLRRDSRGH LGLVANPLKE NLEPFQVSPE SRAVALWQTL QQTTGSESSP
CPLTQLARSL KESAAKVLLR SDCHLSTQAS PLVESEDGSQ VERKGFNPWG LHCHQAHLTR
LCSQYPEDKR HRFLLLENVV SQYKQPCILD LKMGTRQHGD DASEEKKARH MKKCAQSTSA
CLGVRICGMQ VYQTDQKSFL CKDKYYGRKL SVEGFRQALS QFLHDGTRLR AELLEPILRR
LQALLTVIRS QSSYRFYSSS VLIIYDGEPP QTTQGSTSGG VTSGDPAKVD VRMIDFAHTT
FKGSWNEHTT YEGPDPGYIF GLENLIGILR DIQEGE
