IPAC_SHIFL
ID IPAC_SHIFL Reviewed; 363 AA.
AC P18012; Q8VSH7; Q9AJW2;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 31-AUG-2004, sequence version 2.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Invasin IpaC;
DE AltName: Full=42 kDa antigen;
GN Name=ipaC; OrderedLocusNames=CP0127;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, and Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=3057506; DOI=10.1073/pnas.85.23.9317;
RA Venkatesan M.M., Buysse J.M., Kopecko D.J.;
RT "Characterization of invasion plasmid antigen genes (ipaBCD) from Shigella
RT flexneri.";
RL Proc. Natl. Acad. Sci. U.S.A. 85:9317-9321(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=3071655; DOI=10.1016/0882-4010(88)90062-9;
RA Baudry B., Kaczorek M., Sansonetti P.J.;
RT "Nucleotide sequence of the invasion plasmid antigen B and C genes (ipaB
RT and ipaC) of Shigella flexneri.";
RL Microb. Pathog. 4:345-357(1988).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=2552264; DOI=10.1111/j.1365-2958.1989.tb00269.x;
RA Sasakawa C., Adler B., Tobe T., Okada N., Nagai S., Komatsu K.,
RA Yoshikawa M.;
RT "Functional organization and nucleotide sequence of virulence region-2 on
RT the large virulence plasmid in Shigella flexneri 2a.";
RL Mol. Microbiol. 3:1191-1201(1989).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [7]
RP PROTEIN SEQUENCE OF 1-20; 30-45 AND 299-316.
RC STRAIN=Serotype 2a;
RX PubMed=2663721; DOI=10.1128/iai.57.8.2364-2371.1989;
RA Sankaran K., Ramachandran V., Subrahmanyam Y.V.B.K., Rajarathnam S.,
RA Elango S., Roy R.K.;
RT "Congo red-mediated regulation of levels of Shigella flexneri 2a membrane
RT proteins.";
RL Infect. Immun. 57:2364-2371(1989).
RN [8]
RP REVIEW.
RX PubMed=11207575; DOI=10.1046/j.1462-5822.2000.00046.x;
RA Tran Van Nhieu G., Bourdet-Sicard R., Dumenil G., Blocker A.,
RA Sansonetti P.J.;
RT "Bacterial signals and cell responses during Shigella entry into epithelial
RT cells.";
RL Cell. Microbiol. 2:187-193(2000).
CC -!- FUNCTION: Forms a pore with IpaB, which is inserted into host cell
CC membrane through the Mxi/Spa apparatus, during cell contact. This pore
CC probably allows the translocation of IpaA. The C-terminus of IpaC could
CC be involved in actin polymerization that leads to the cell extension
CC formation and its N-terminus could be involved in the conversion of
CC filopodial extensions into lamellipodial extension. Most extensions
CC results from Cdc42 activation by IpaC whereas the lamellipodial
CC extensions result from the Rac activation by Cdc42.
CC -!- INTERACTION:
CC P18012; P35222: CTNNB1; Xeno; NbExp=4; IntAct=EBI-491541, EBI-491549;
CC -!- SUBCELLULAR LOCATION: Secreted. Note=Secreted through the specialized
CC type-III secretion system Mxi/Spa. Inserted into the host cell
CC membrane.
CC -!- INDUCTION: Synthesis of this immunogen is repressed at 30 degrees
CC Celsius and restored at 37 degrees Celsius.
CC -!- SIMILARITY: Belongs to the invasin protein C family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA26523.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAK18445.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA33382.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; J04117; AAA26523.1; ALT_INIT; Genomic_DNA.
DR EMBL; M34849; AAA98425.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05802.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18445.1; ALT_INIT; Genomic_DNA.
DR EMBL; X15319; CAA33382.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF386526; AAL72351.2; -; Genomic_DNA.
DR PIR; C31265; A60112.
DR RefSeq; NP_858260.2; NC_004851.1.
DR RefSeq; WP_031942475.1; NZ_QWST01000007.1.
DR RefSeq; YP_009062484.1; NC_024996.1.
DR AlphaFoldDB; P18012; -.
DR IntAct; P18012; 1.
DR STRING; 198214.CP0127; -.
DR TCDB; 1.C.36.3.1; the bacterial type iii-target cell pore (iiitcp) family.
DR PRIDE; P18012; -.
DR EnsemblBacteria; AAL72351; AAL72351; SF_p0127.
DR GeneID; 1238054; -.
DR KEGG; sfl:CP0127; -.
DR PATRIC; fig|198214.7.peg.5382; -.
DR HOGENOM; CLU_723395_0_0_6; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR InterPro; IPR005427; BacInvasinC.
DR Pfam; PF09599; IpaC_SipC; 1.
DR TIGRFAMs; TIGR02101; IpaC_SipC; 1.
PE 1: Evidence at protein level;
KW Actin-binding; Direct protein sequencing; Plasmid; Reference proteome;
KW Secreted; Virulence.
FT CHAIN 1..363
FT /note="Invasin IpaC"
FT /id="PRO_0000219858"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 11
FT /note="T -> I (in plasmid pMYSH6000 and plasmid pCP301)"
FT VARIANT 84
FT /note="L -> R (in plasmid pCP301)"
FT VARIANT 353
FT /note="A -> T (in plasmid pMYSH6000 and plasmid pCP301)"
FT CONFLICT 17
FT /note="S -> E (in Ref. 7; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="Q -> E (in Ref. 7; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 38776 MW; AE82D80C7501D289 CRC64;
MEIQNTKPTQ TLYTDISTKQ TQSSSETQKS QNYQQIAAHI PLNVGKNPVL TTTLNDDQLL
KLSEQVQHDS EIIARLTDKK MKDLSEMSHT LTPENTLDIS SLSSNAVSLI ISVAVLLSAL
RTAETKLGSQ LSLIAFDATK SAAENIVRQG LAALSSSITG AVTQVGITGI GAKKTHSGIS
DQKGALRKNL ATAQSLEKEL AGSKLGLNKQ IDTNITSPQT NSSTKFLGKN KLAPDNISLS
TEHKTSLSSP DISLQDKIDT QRRTYELNTL SAQQKQNIGR ATMETSAVAG NISTSGGRYA
SALEEEEQLI SQASSKQAEE ASQVSKEASQ ATNQLIQKLL NIIDSINQSK NSAASQIAGN
IRA
