IPDB_MYCBO
ID IPDB_MYCBO Reviewed; 250 AA.
AC P63653; A0A1R3Y4K3; P71848; X2BNK8;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 25-MAY-2022, entry version 82.
DE RecName: Full=Cholesterol ring-cleaving hydrolase IpdB subunit {ECO:0000250|UniProtKB:P9WPV9};
DE EC=4.1.99.- {ECO:0000250|UniProtKB:P9WPV9};
DE AltName: Full=(3E)-2-(2-carboxylatoethyl)-3-methyl-6-oxocyclohex-1-ene-1-carboxyl-CoA hydrolase beta subunit {ECO:0000250|UniProtKB:P9WPV9};
DE Short=COCHEA-CoA hydrolase beta subunit {ECO:0000250|UniProtKB:P9WPV9};
GN Name=ipdB {ECO:0000250|UniProtKB:P9WPV9}; OrderedLocusNames=BQ2027_MB3582;
OS Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=233413;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT "The complete genome sequence of Mycobacterium bovis.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=ATCC BAA-935 / AF2122/97;
RX PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA Robbe-Austerman S., Gordon S.V.;
RT "Updated reference genome sequence and annotation of Mycobacterium bovis
RT AF2122/97.";
RL Genome Announc. 5:E00157-E00157(2017).
CC -!- FUNCTION: Involved in the final steps of cholesterol and steroid
CC degradation. Opens the last steroid ring of cholesterol by catalyzing
CC the hydrolysis of (3E)-2-(2-carboxylatoethyl)-3-methyl-6-oxocyclohex-1-
CC ene-1-carboxyl-CoA (COCHEA-CoA) to 6-methyl-3,7-dioxodecanedioyl-CoA
CC (MeDODA-CoA). {ECO:0000250|UniProtKB:P9WPV9}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3E)-2-(2-carboxylatoethyl)-3-methyl-6-oxocyclohex-1-ene-1-
CC carboxyl-CoA + H2O = 6-methyl-3,7-dioxodecanedioyl-CoA;
CC Xref=Rhea:RHEA:66364, ChEBI:CHEBI:15377, ChEBI:CHEBI:167101,
CC ChEBI:CHEBI:167102; Evidence={ECO:0000250|UniProtKB:P9WPV9};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:66365;
CC Evidence={ECO:0000250|UniProtKB:P9WPV9};
CC -!- PATHWAY: Steroid metabolism; cholesterol degradation.
CC {ECO:0000250|UniProtKB:P9WPV9}.
CC -!- SUBUNIT: Heterotetramer composed of 2 IpdA subunits and 2 IpdB
CC subunits. {ECO:0000250|UniProtKB:P9WPV9}.
CC -!- SIMILARITY: Belongs to the 3-oxoacid CoA-transferase subunit B family.
CC {ECO:0000305}.
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DR EMBL; LT708304; SIU02209.1; -; Genomic_DNA.
DR RefSeq; NP_857221.1; NC_002945.3.
DR RefSeq; WP_003419321.1; NC_002945.4.
DR AlphaFoldDB; P63653; -.
DR SMR; P63653; -.
DR EnsemblBacteria; SIU02209; SIU02209; BQ2027_MB3582.
DR GeneID; 45427536; -.
DR PATRIC; fig|233413.5.peg.3925; -.
DR OMA; YWVGNHS; -.
DR UniPathway; UPA01058; -.
DR Proteomes; UP000001419; Chromosome.
DR GO; GO:0008410; F:CoA-transferase activity; IEA:InterPro.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0006707; P:cholesterol catabolic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR004165; CoA_trans_fam_I.
DR InterPro; IPR037171; NagB/RpiA_transferase-like.
DR SMART; SM00882; CoA_trans; 1.
DR SUPFAM; SSF100950; SSF100950; 1.
PE 3: Inferred from homology;
KW Cholesterol metabolism; Lipid metabolism; Lyase; Steroid metabolism;
KW Sterol metabolism.
FT CHAIN 1..250
FT /note="Cholesterol ring-cleaving hydrolase IpdB subunit"
FT /id="PRO_0000157932"
SQ SEQUENCE 250 AA; 27393 MW; 4278D0CDCBDCF6DE CRC64;
MSTRAEVCAV ACAELFRDAG EIMISPMTNM ASVGARLARL TFAPDILLTD GEAQLLADTP
ALGKTGAPNR IEGWMPFGRV FETLAWGRRH VVMGANQVDR YGNQNISAFG PLQRPTRQMF
GVRGSPGNTI NHATSYWVGN HCKRVFVEAV DVVSGIGYDK VDPDNPAFRF VNVYRVVSNL
GVFDFGGPDH SMRAVSLHPG VTPGDVRDAT SFEVHDLDAA EQTRLPTDDE LHLIRAVIDP
KSLRDREIRS