ID   IPDE_DICDI              Reviewed;         237 AA.
AC   P22549; Q54YU3;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Cyclic nucleotide phosphodiesterase inhibitor;
DE            Short=PDI;
DE   Flags: Precursor;
GN   Name=pdiA; Synonyms=pdi; ORFNames=DDB_G0277863;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX3;
RX   PubMed=2169446; DOI=10.1016/0378-1119(90)90161-j;
RA   Wu L., Franke J.;
RT   "A developmentally regulated and cAMP-repressible gene of Dictyostelium
RT   discoideum: cloning and expression of the gene encoding cyclic nucleotide
RT   phosphodiesterase inhibitor.";
RL   Gene 91:51-56(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX   PubMed=2049870; DOI=10.1002/dvg.1020120118;
RA   Franke J., Faure M., Wu L., Hall A.L., Podgorski G.J., Kessin R.H.;
RT   "Cyclic nucleotide phosphodiesterase of Dictyostelium discoideum and its
RT   glycoprotein inhibitor: structure and expression of their genes.";
RL   Dev. Genet. 12:104-112(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: PDI acts by binding stoichiometrically to cyclic nucleotide
CC       phosphodiesterase, changing the KM of the enzyme for cAMP from 10 uM to
CC       2 mM.
CC   -!- DEVELOPMENTAL STAGE: Expressed early in development with little
CC       transcript remaining following aggregation.
CC   -!- INDUCTION: By starvation. Repressed by cAMP.
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DR   EMBL; X16057; CAA34193.1; -; Genomic_DNA.
DR   EMBL; AAFI02000023; EAL68104.1; -; Genomic_DNA.
DR   PIR; JQ0684; S08073.
DR   RefSeq; XP_642109.1; XM_637017.1.
DR   AlphaFoldDB; P22549; -.
DR   STRING; 44689.DDB0214948; -.
DR   PaxDb; P22549; -.
DR   EnsemblProtists; EAL68104; EAL68104; DDB_G0277863.
DR   GeneID; 8621320; -.
DR   KEGG; ddi:DDB_G0277863; -.
DR   dictyBase; DDB_G0277863; pdiA.
DR   eggNOG; ENOG502RCST; Eukaryota.
DR   HOGENOM; CLU_102388_0_0_1; -.
DR   InParanoid; P22549; -.
DR   OMA; ADCHCTK; -.
DR   PhylomeDB; P22549; -.
DR   PRO; PR:P22549; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR   GO; GO:0004857; F:enzyme inhibitor activity; IDA:dictyBase.
DR   GO; GO:0031153; P:slug development involved in sorocarp development; IMP:dictyBase.
DR   InterPro; IPR001673; S_mold_repeat.
DR   Pfam; PF00526; Dicty_CTDC; 4.
PE   1: Evidence at protein level;
KW   Developmental protein; Direct protein sequencing; Glycoprotein;
KW   Reference proteome; Repeat; Signal; Stress response.
FT   SIGNAL          1..20
FT   CHAIN           21..237
FT                   /note="Cyclic nucleotide phosphodiesterase inhibitor"
FT                   /id="PRO_0000021517"
FT   REPEAT          57..81
FT                   /note="Cys-rich CT 1"
FT   REPEAT          82..105
FT                   /note="Cys-rich CT 2"
FT   REPEAT          116..139
FT                   /note="Cys-rich CT 3"
FT   REPEAT          140..162
FT                   /note="Cys-rich CT 4"
FT   REPEAT          163..186
FT                   /note="Cys-rich CT 5"
FT   CARBOHYD        28
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        65
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        70
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        153
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        207
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   237 AA;  25757 MW;  675198A50B9EB7D9 CRC64;
     MAKIIISLIL LLSLFSFSYG AYNCNKLNCS SKNTKCRTFS CTSVVGCYYT DKCTSPDLCH
     NSACNASTGN CTLTTISCND NNPCTDDFCH PGYGCYSVPN SCDPGVICQQ NCNDNDPCTY
     DFCDALNICR HSETYCNDGD ACTLNTCGVN GCNFTKISCD DNDPCTADYC STLYGCYHEP
     IECSIKVPCN IDSDCNRNNG CETFTCNLST NTCDYYAKNC GGWPCINNQC TTGSISN