IPI1_ORYSJ
ID IPI1_ORYSJ Reviewed; 455 AA.
AC Q5Z8R1; B7EHI9; Q0JMY2;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=E3 ubiquitin-protein ligase IPI1 {ECO:0000305};
DE EC=2.3.2.27 {ECO:0000269|PubMed:28298520};
DE AltName: Full=IPA1-interacting protein 1 {ECO:0000303|PubMed:28298520};
GN Name=IPI1 {ECO:0000303|PubMed:28298520};
GN OrderedLocusNames=Os01g0350900 {ECO:0000312|EMBL:BAS72030.1},
GN LOC_Os01g24880 {ECO:0000305};
GN ORFNames=B1051E10.48-1 {ECO:0000312|EMBL:BAD53853.1},
GN P0463A02.18-1 {ECO:0000312|EMBL:BAD52927.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP FUNCTION, CATALYTIC ACTIVITY, INTERACTION WITH SPL14, SUBCELLULAR LOCATION,
RP MUTAGENESIS OF HIS-74 AND CYS-80, AND DISRUPTION PHENOTYPE.
RX PubMed=28298520; DOI=10.1105/tpc.16.00879;
RA Wang J., Yu H., Xiong G., Lu Z., Jiao Y., Meng X., Liu G., Chen X.,
RA Wang Y., Li J.;
RT "Tissue-specific ubiquitination by IPA1 INTERACTING PROTEIN1 modulates IPA1
RT protein levels to regulate plant architecture in rice.";
RL Plant Cell 29:697-707(2017).
CC -!- FUNCTION: Functions as an E3 ligase that promotes polyubiquitination of
CC SPL14/IPA1 for subsequent proteasomal degradation (PubMed:28298520).
CC Regulates plant architecture by modulating SPL14/IPA1 abundance
CC (PubMed:28298520). Promotes the degradation of SPL14/IPA1 in panicles,
CC while it stabilizes SPL14/IPA1 in shoot apices (PubMed:28298520).
CC Ubiquitinates the SPL14/IPA1-mediated complex with 'Lys-48'-linked
CC polyubiquitin in panicles and 'Lys-63'-linked polyubiquitin chains in
CC the shoot apex (PubMed:28298520). {ECO:0000269|PubMed:28298520}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000269|PubMed:28298520};
CC -!- PATHWAY: Protein modification; protein ubiquitination. {ECO:0000305}.
CC -!- SUBUNIT: Interacts with SPL14/IPA1. {ECO:0000269|PubMed:28298520}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:28298520}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5Z8R1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5Z8R1-2; Sequence=VSP_061157;
CC -!- DISRUPTION PHENOTYPE: Altered plant architecture, including increased
CC number of tillers, enlarged panicles, and increased grain yield per
CC plant. {ECO:0000269|PubMed:28298520}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF04896.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AP003258; BAD52927.1; -; Genomic_DNA.
DR EMBL; AP003764; BAD53853.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF04896.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014957; BAS72030.1; -; Genomic_DNA.
DR EMBL; AK070217; BAG91836.1; -; mRNA.
DR EnsemblPlants; Os01t0350900-01; Os01t0350900-01; Os01g0350900. [Q5Z8R1-2]
DR EnsemblPlants; Os01t0350900-02; Os01t0350900-02; Os01g0350900. [Q5Z8R1-1]
DR Gramene; Os01t0350900-01; Os01t0350900-01; Os01g0350900. [Q5Z8R1-2]
DR Gramene; Os01t0350900-02; Os01t0350900-02; Os01g0350900. [Q5Z8R1-1]
DR HOGENOM; CLU_037685_0_1_1; -.
DR InParanoid; Q5Z8R1; -.
DR PlantReactome; R-OSA-9030908; Underwater shoot and internode elongation.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q5Z8R1; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR GO; GO:0044260; P:cellular macromolecule metabolic process; IEA:UniProt.
DR GO; GO:0048450; P:floral organ structural organization; IMP:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR GO; GO:0080050; P:regulation of seed development; IMP:UniProtKB.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR044274; RFI2.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR46798; PTHR46798; 1.
DR Pfam; PF13639; zf-RING_2; 1.
DR SMART; SM00184; RING; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Metal-binding; Nucleus; Reference proteome;
KW Transferase; Ubl conjugation pathway; Zinc; Zinc-finger.
FT CHAIN 1..455
FT /note="E3 ubiquitin-protein ligase IPI1"
FT /id="PRO_0000453486"
FT ZN_FING 51..97
FT /note="RING-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 286..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 426..455
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 288..309
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 304..455
FT /note="Missing (in isoform 2)"
FT /id="VSP_061157"
FT MUTAGEN 74
FT /note="H->Y: Loss of E3 ubiquitin-protein ligase activity."
FT /evidence="ECO:0000269|PubMed:28298520"
FT MUTAGEN 80
FT /note="C->S: Loss of E3 ubiquitin-protein ligase activity."
FT /evidence="ECO:0000269|PubMed:28298520"
SQ SEQUENCE 455 AA; 48894 MW; 31ECDF03DAD6ECBD CRC64;
MGAEEEEEPA SAVGREGGGG GGGARAAGAG AGGDTADDDD SGESAAAVVP CSICLDAVVA
GGGDRSTARL QCGHEFHLDC IGSAFNAKGV MQCPNCRQIE RGNWLYANGS RPSQDVSNDD
WGHDEDFYDA NQPETSRSVF LPFRFQWCPI GRLAQLPSVF DEGESAPPVT FHDFMGQNFT
SEHLPVSAPG ATPPGPYIAY FQPLQSSASS SSSHVTERTM DGTTYHDHWN PLPGPSDGRP
LATVHPIDFH HNHWTHLPNS YSQPNSNNGV AEQMAIPVVP MRVGGLDSDS QQRGSLPSVY
GNGSGSRSRI PSVPPMAPQF MRPHGNINEQ YQQNSSSLYA APQRRTAVQA VQDSMNFTLF
PQAPTGPNSM ETEDAGGNQF YAWERDRFAP YPLMPVDSEA NWWGSTPQSH GVTDHSAAPG
RRLFGQWIGA GRSPPPPPPP PADNSSYRQM HIPRM
