ID   IPIA_TOBAC              Reviewed;         107 AA.
AC   Q03198;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Proteinase inhibitor I-A;
DE            Short=PI-IA;
DE   AltName: Full=Inhibitor of microbial serine proteinases minor isoform;
DE   Flags: Precursor;
GN   Name=TIMPB;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8490144; DOI=10.1007/bf00023597;
RA   Linthorst H.J.M., Brederode F.T., van der Does C., Bol J.F.;
RT   "Tobacco proteinase inhibitor I genes are locally, but not systemically
RT   induced by stress.";
RL   Plant Mol. Biol. 21:985-992(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Samsun NN; TISSUE=Leaf;
RX   PubMed=8102364; DOI=10.1016/s0021-9258(19)85291-2;
RA   Heitz T., Geoffroy P., Stintzi A., Fritig B., Legrand M.;
RT   "cDNA cloning and gene expression analysis of the microbial proteinase
RT   inhibitor of tobacco.";
RL   J. Biol. Chem. 268:16987-16992(1993).
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- INDUCTION: By tobacco mosaic virus infection, wounding, UV light,
CC       salicylic acid and ethylene.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I13 (potato type I serine
CC       protease inhibitor) family. {ECO:0000305}.
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DR   EMBL; Z12619; CAA78265.1; -; mRNA.
DR   EMBL; X67075; CAA47460.1; -; mRNA.
DR   PIR; S33546; B47487.
DR   RefSeq; XP_016464920.1; XM_016609434.1.
DR   AlphaFoldDB; Q03198; -.
DR   SMR; Q03198; -.
DR   GeneID; 107787825; -.
DR   KEGG; nta:107787825; -.
DR   OMA; MDKLAHF; -.
DR   OrthoDB; 1582946at2759; -.
DR   PhylomeDB; Q03198; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   GO; GO:0009611; P:response to wounding; IEA:InterPro.
DR   InterPro; IPR000864; Prot_inh_pot1.
DR   InterPro; IPR036354; Prot_inh_pot1_sf.
DR   PANTHER; PTHR33091; PTHR33091; 1.
DR   Pfam; PF00280; potato_inhibit; 1.
DR   PRINTS; PR00292; POTATOINHBTR.
DR   SUPFAM; SSF54654; SSF54654; 1.
DR   PROSITE; PS00285; POTATO_INHIBITOR; 1.
PE   2: Evidence at transcript level;
KW   Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..36
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000025304"
FT   CHAIN           37..107
FT                   /note="Proteinase inhibitor I-A"
FT                   /id="PRO_0000025305"
FT   SITE            83..84
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   107 AA;  11880 MW;  9348A7C3344E87AE CRC64;
     MVKFAHVVAF LLLASLIQPL TARDLEINVL QLDVSQSGCP GVTKERWPEL LGTPAKFAMQ
     IIQKENPKLT NVQTILNGGP VTEDLRCNRV RLFVNVLDFI VQTPQIG