ID   IPK1_ASHGO              Reviewed;         278 AA.
AC   Q75EW5;
DT   26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Inositol-pentakisphosphate 2-kinase;
DE            EC=2.7.1.158;
DE   AltName: Full=Inositol-1,3,4,5,6-pentakisphosphate 2-kinase;
DE   AltName: Full=Ins(1,3,4,5,6)P5 2-kinase;
DE            Short=InsP5 2-kinase;
GN   Name=IPK1; OrderedLocusNames=AAL037C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Has kinase activity and phosphorylates inositol-1,3,4,5,6-
CC       pentakisphosphate (Ins(1,3,4,5,6)P5) to produce 1,2,3,4,5,6-
CC       hexakisphosphate (InsP6), also known as phytate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP = 1D-myo-
CC         inositol hexakisphosphate + ADP + H(+); Xref=Rhea:RHEA:20313,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57733,
CC         ChEBI:CHEBI:58130, ChEBI:CHEBI:456216; EC=2.7.1.158;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- DOMAIN: The EXKPK motif is conserved in inositol-pentakisphosphate 2-
CC       kinases of both family 1 and 2.
CC   -!- SIMILARITY: Belongs to the IPK1 type 1 family. {ECO:0000305}.
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DR   EMBL; AE016814; AAS50329.1; -; Genomic_DNA.
DR   RefSeq; NP_982505.1; NM_207858.1.
DR   AlphaFoldDB; Q75EW5; -.
DR   SMR; Q75EW5; -.
DR   STRING; 33169.AAS50329; -.
DR   EnsemblFungi; AAS50329; AAS50329; AGOS_AAL037C.
DR   GeneID; 4618627; -.
DR   KEGG; ago:AGOS_AAL037C; -.
DR   eggNOG; ENOG502S05I; Eukaryota.
DR   HOGENOM; CLU_046294_0_0_1; -.
DR   InParanoid; Q75EW5; -.
DR   OMA; NDYLRHK; -.
DR   Proteomes; UP000000591; Chromosome I.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0035299; F:inositol pentakisphosphate 2-kinase activity; IBA:GO_Central.
DR   GO; GO:0032958; P:inositol phosphate biosynthetic process; IBA:GO_Central.
DR   GO; GO:0052746; P:inositol phosphorylation; IBA:GO_Central.
DR   GO; GO:0070481; P:nuclear-transcribed mRNA catabolic process, non-stop decay; IEA:EnsemblFungi.
DR   InterPro; IPR009286; Ins_P5_2-kin.
DR   PANTHER; PTHR14456; PTHR14456; 1.
DR   Pfam; PF06090; Ins_P5_2-kin; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Kinase; Nucleotide-binding; Nucleus; Reference proteome;
KW   Transferase.
FT   CHAIN           1..278
FT                   /note="Inositol-pentakisphosphate 2-kinase"
FT                   /id="PRO_0000110521"
FT   MOTIF           123..127
FT                   /note="EXKPK motif"
SQ   SEQUENCE   278 AA;  31047 MW;  E2E62A8512B1F43A CRC64;
     MVKIIGKGGA NYVLAFGNDN DDLYRICVRG RSLRENNRST VDNYHYALEV VKPQLGEFVC
     RMELVDLPVC DSLRQVLKSK IEVWDATTVT CLKMPNLVPA GSLSHTVDHF TKIHIGERAI
     VWEFKPKWLS GNDKYCRNCT LNLLRGQTSI SYCHAQLLNP GQAGPILKSL FAGLNVPPAF
     IEDMEAYIAQ PCSVLQRLRV AQEQVDASLG PLDQPDTAAS PERCLSMTLK DVSCFVSWHK
     DASPVAVVVD LDMKPAAKSA HWTALQEQLD RFQPQVRH