IPK2_ORYSJ
ID IPK2_ORYSJ Reviewed; 295 AA.
AC Q6H545; A0A0P0VJQ8;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Inositol polyphosphate multikinase IPK2 {ECO:0000305};
DE Short=OsIPK2 {ECO:0000303|PubMed:17961936};
DE EC=2.7.1.140 {ECO:0000305};
DE EC=2.7.1.151 {ECO:0000305};
DE AltName: Full=Inositol polyphosphate 6-/3-/5-kinase;
GN Name=IPK2 {ECO:0000303|PubMed:17961936};
GN OrderedLocusNames=Os02g0523800 {ECO:0000312|EMBL:BAF08900.1},
GN LOC_Os02g32370 {ECO:0000305};
GN ORFNames=OsJ_06957 {ECO:0000312|EMBL:EAZ23262.1},
GN OSJNBa0047A17.37 {ECO:0000312|EMBL:BAD26154.1},
GN P0415B12.9 {ECO:0000312|EMBL:BAD25378.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Zhou T., Zhang Z.-B., Xia H.-J.;
RT "Isolation and identification of inositol polyphosphate kinase gene from
RT Oryza sativa.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=17961936; DOI=10.1016/j.gene.2007.09.006;
RA Suzuki M., Tanaka K., Kuwano M., Yoshida K.T.;
RT "Expression pattern of inositol phosphate-related enzymes in rice (Oryza
RT sativa L.): implications for the phytic acid biosynthetic pathway.";
RL Gene 405:55-64(2007).
CC -!- FUNCTION: Inositol phosphate kinase with a broad substrate specificity.
CC Phosphorylates inositol 1,4,5-trisphosphate (Ins(1,4,5)P3), inositol
CC 1,4,5,6-tetrakisphosphate (Ins(1,4,5,6)P4), inositol 1,3,4,5-
CC tetrakisphosphate (Ins(1,3,4,5)P4), inositol 1,3,4,6-tetrakisphosphate
CC (Ins(1,3,4,6)P4) and inositol 1,2,3,4,6-pentakisphosphate
CC (Ins(1,2,3,4,6)P5) but not inositol 1,4-bisphosphate (Ins(1,4)P2),
CC inositol 1,3,4-trisphosphate (Ins(1,3,4)P3), inositol 1,2,6-
CC trisphosphate (Ins(1,2,6)P3), inositol 3,4,5,6-tetrakisphosphate
CC (Ins(3,4,5,6)P4), inositol 1,3,4,5,6-pentakisphosphate
CC (Ins(1,3,4,5,6)P5), inositol 1,2,4,5,6-pentakisphosphate
CC (Ins(1,2,4,5,6)P5) or inositol hexakisphosphate (InsP6). Regulates
CC pollen and root development probably through the regulation of InsP3-
CC mediated calcium accumulation. {ECO:0000250|UniProtKB:Q9LY23}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1D-myo-inositol 1,4,5-trisphosphate + 2 ATP = 1D-myo-inositol
CC 1,3,4,5,6-pentakisphosphate + 2 ADP + 2 H(+); Xref=Rhea:RHEA:32359,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57733,
CC ChEBI:CHEBI:203600, ChEBI:CHEBI:456216; EC=2.7.1.151;
CC Evidence={ECO:0000250|UniProtKB:Q9LY23};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1D-myo-inositol 1,3,4,6-tetrakisphosphate + ATP = 1D-myo-
CC inositol 1,3,4,5,6-pentakisphosphate + ADP + H(+);
CC Xref=Rhea:RHEA:12717, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:57660, ChEBI:CHEBI:57733, ChEBI:CHEBI:456216;
CC EC=2.7.1.140; Evidence={ECO:0000250|UniProtKB:Q9LY23};
CC -!- TISSUE SPECIFICITY: Highly expressed in anthers and at lower levels in
CC roots, leaves, flowers and embryos. {ECO:0000269|PubMed:17961936}.
CC -!- SIMILARITY: Belongs to the inositol phosphokinase (IPK) family.
CC {ECO:0000305}.
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DR EMBL; AY529102; AAS93936.1; -; mRNA.
DR EMBL; AP004772; BAD25378.1; -; Genomic_DNA.
DR EMBL; AP005749; BAD26154.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF08900.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS78971.1; -; Genomic_DNA.
DR EMBL; CM000139; EAZ23262.1; -; Genomic_DNA.
DR RefSeq; XP_015623153.1; XM_015767667.1.
DR AlphaFoldDB; Q6H545; -.
DR SMR; Q6H545; -.
DR STRING; 4530.OS02T0523800-01; -.
DR PaxDb; Q6H545; -.
DR PRIDE; Q6H545; -.
DR EnsemblPlants; Os02t0523800-01; Os02t0523800-01; Os02g0523800.
DR GeneID; 4329509; -.
DR Gramene; Os02t0523800-01; Os02t0523800-01; Os02g0523800.
DR KEGG; osa:4329509; -.
DR eggNOG; KOG1620; Eukaryota.
DR HOGENOM; CLU_042569_1_2_1; -.
DR InParanoid; Q6H545; -.
DR OMA; KPECGER; -.
DR OrthoDB; 902814at2759; -.
DR PlantReactome; R-OSA-1119434; Phytic acid biosynthesis (lipid-independent).
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6H545; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0047326; F:inositol tetrakisphosphate 5-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0051765; F:inositol tetrakisphosphate kinase activity; IBA:GO_Central.
DR GO; GO:0008440; F:inositol-1,4,5-trisphosphate 3-kinase activity; IBA:GO_Central.
DR GO; GO:0000823; F:inositol-1,4,5-trisphosphate 6-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0016301; F:kinase activity; IBA:GO_Central.
DR GO; GO:0102732; F:myo-inositol-1,2,3,4,6-heptakisphosphate 5-kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0032958; P:inositol phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.470.160; -; 1.
DR InterPro; IPR005522; IPK.
DR InterPro; IPR038286; IPK_sf.
DR PANTHER; PTHR12400; PTHR12400; 1.
DR Pfam; PF03770; IPK; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..295
FT /note="Inositol polyphosphate multikinase IPK2"
FT /id="PRO_0000431883"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 295 AA; 31029 MW; 97932BDB26C65FCC CRC64;
MASDLRPPEH QVAGHRASAD KLGPLVDGEG LFYKPLQAGE RGEHEAAFYA AFTAHPAVPP
RVRGAFFPRF HGTRFLPAPA SPGGAPYPHI VLDDLLAGLP SPCVADVKIG ACTWPPRSPD
PYVAKCLAKD RETTSALLGF RVSGVRVVDA RGGAVWRPDR SELKGIDAAG VRRVLRRYVS
TGGGDGLDCA LAAAVYGGEG GVLAQLRELK AWFEEQTLYH FYSASILFGY DANAAAAAAP
GGGSGGVRVK LVDFAHVDDG DGVIDHNFLG GLCSLIKFIG DIVAEVTEKA SSDHS
