IPKB_HUMAN
ID IPKB_HUMAN Reviewed; 78 AA.
AC Q9C010; B2RCK2; Q567T9; Q5T0Z7;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=cAMP-dependent protein kinase inhibitor beta;
DE Short=PKI-beta;
GN Name=PKIB; Synonyms=PRKACN2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=10880337; DOI=10.1042/0264-6021:3490403;
RA Zheng L., Yu L., Tu Q., Zhang M., He H., Chen W., Gao J., Yu J., Wu Q.,
RA Zhao S.;
RT "Cloning and mapping of human PKIB and PKIG, and comparison of tissue
RT expression patterns of three members of the protein kinase inhibitor
RT family, including PKIA.";
RL Biochem. J. 349:403-407(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Subthalamic nucleus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Extremely potent competitive inhibitor of cAMP-dependent
CC protein kinase activity, this protein interacts with the catalytic
CC subunit of the enzyme after the cAMP-induced dissociation of its
CC regulatory chains. {ECO:0000250}.
CC -!- INTERACTION:
CC Q9C010-2; Q9BYR8: KRTAP3-1; NbExp=3; IntAct=EBI-12886396, EBI-9996449;
CC Q9C010-2; P17612: PRKACA; NbExp=3; IntAct=EBI-12886396, EBI-476586;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C010-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C010-2; Sequence=VSP_046922;
CC -!- SIMILARITY: Belongs to the PKI family. {ECO:0000305}.
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DR EMBL; AF225513; AAK00638.1; -; mRNA.
DR EMBL; AK315151; BAG37599.1; -; mRNA.
DR EMBL; AF087873; AAM10501.1; -; mRNA.
DR EMBL; AL512283; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL513481; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z99129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471051; EAW48171.1; -; Genomic_DNA.
DR EMBL; BC036011; AAH36011.1; -; mRNA.
DR EMBL; BC093027; AAH93027.1; -; mRNA.
DR CCDS; CCDS5126.1; -. [Q9C010-1]
DR CCDS; CCDS59033.1; -. [Q9C010-2]
DR RefSeq; NP_001257322.1; NM_001270393.1. [Q9C010-1]
DR RefSeq; NP_001257323.1; NM_001270394.1. [Q9C010-2]
DR RefSeq; NP_001257324.1; NM_001270395.1. [Q9C010-2]
DR RefSeq; NP_115860.1; NM_032471.5. [Q9C010-1]
DR RefSeq; NP_861459.1; NM_181794.2. [Q9C010-1]
DR RefSeq; NP_861460.1; NM_181795.2. [Q9C010-1]
DR RefSeq; XP_011534232.1; XM_011535930.2. [Q9C010-2]
DR RefSeq; XP_011534233.1; XM_011535931.2. [Q9C010-2]
DR RefSeq; XP_011534234.1; XM_011535932.2. [Q9C010-2]
DR RefSeq; XP_011534235.1; XM_011535933.2. [Q9C010-2]
DR RefSeq; XP_011534236.1; XM_011535934.2.
DR RefSeq; XP_011534237.1; XM_011535935.2. [Q9C010-1]
DR RefSeq; XP_011534238.1; XM_011535936.1. [Q9C010-1]
DR RefSeq; XP_011534239.1; XM_011535937.2. [Q9C010-1]
DR AlphaFoldDB; Q9C010; -.
DR BioGRID; 111557; 7.
DR IntAct; Q9C010; 5.
DR STRING; 9606.ENSP00000480824; -.
DR iPTMnet; Q9C010; -.
DR PhosphoSitePlus; Q9C010; -.
DR BioMuta; PKIB; -.
DR EPD; Q9C010; -.
DR MassIVE; Q9C010; -.
DR MaxQB; Q9C010; -.
DR PaxDb; Q9C010; -.
DR PeptideAtlas; Q9C010; -.
DR PRIDE; Q9C010; -.
DR ProteomicsDB; 79940; -. [Q9C010-1]
DR Antibodypedia; 32638; 114 antibodies from 20 providers.
DR DNASU; 5570; -.
DR Ensembl; ENST00000258014.3; ENSP00000258014.3; ENSG00000135549.15. [Q9C010-2]
DR Ensembl; ENST00000354275.2; ENSP00000346227.2; ENSG00000135549.15. [Q9C010-1]
DR Ensembl; ENST00000368448.5; ENSP00000357433.1; ENSG00000135549.15. [Q9C010-1]
DR Ensembl; ENST00000368452.7; ENSP00000357437.2; ENSG00000135549.15. [Q9C010-1]
DR Ensembl; ENST00000392490.5; ENSP00000376280.1; ENSG00000135549.15. [Q9C010-1]
DR Ensembl; ENST00000392491.6; ENSP00000376281.1; ENSG00000135549.15. [Q9C010-1]
DR Ensembl; ENST00000615438.4; ENSP00000480824.1; ENSG00000135549.15. [Q9C010-2]
DR GeneID; 5570; -.
DR KEGG; hsa:5570; -.
DR MANE-Select; ENST00000368452.7; ENSP00000357437.2; NM_181795.3; NP_861460.1.
DR UCSC; uc003pyz.4; human. [Q9C010-1]
DR CTD; 5570; -.
DR DisGeNET; 5570; -.
DR GeneCards; PKIB; -.
DR HGNC; HGNC:9018; PKIB.
DR HPA; ENSG00000135549; Tissue enhanced (brain, intestine, parathyroid gland, placenta).
DR MIM; 606914; gene.
DR neXtProt; NX_Q9C010; -.
DR OpenTargets; ENSG00000135549; -.
DR PharmGKB; PA33350; -.
DR VEuPathDB; HostDB:ENSG00000135549; -.
DR eggNOG; ENOG502S8BW; Eukaryota.
DR GeneTree; ENSGT00530000064264; -.
DR HOGENOM; CLU_163471_1_0_1; -.
DR InParanoid; Q9C010; -.
DR OMA; DAAMRKD; -.
DR OrthoDB; 1648219at2759; -.
DR PhylomeDB; Q9C010; -.
DR TreeFam; TF330809; -.
DR PathwayCommons; Q9C010; -.
DR SignaLink; Q9C010; -.
DR BioGRID-ORCS; 5570; 12 hits in 1077 CRISPR screens.
DR ChiTaRS; PKIB; human.
DR GeneWiki; PKIB; -.
DR GenomeRNAi; 5570; -.
DR Pharos; Q9C010; Tbio.
DR PRO; PR:Q9C010; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q9C010; protein.
DR Bgee; ENSG00000135549; Expressed in cerebellar vermis and 143 other tissues.
DR ExpressionAtlas; Q9C010; baseline and differential.
DR Genevisible; Q9C010; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004862; F:cAMP-dependent protein kinase inhibitor activity; IBA:GO_Central.
DR GO; GO:2000480; P:negative regulation of cAMP-dependent protein kinase activity; IBA:GO_Central.
DR GO; GO:0051973; P:positive regulation of telomerase activity; IMP:BHF-UCL.
DR GO; GO:1904355; P:positive regulation of telomere capping; IMP:BHF-UCL.
DR GO; GO:0032212; P:positive regulation of telomere maintenance via telomerase; IMP:BHF-UCL.
DR InterPro; IPR004171; cAMP_dep_PKI.
DR PANTHER; PTHR15416; PTHR15416; 1.
DR Pfam; PF02827; PKI; 1.
DR PIRSF; PIRSF001667; PKI; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Protein kinase inhibitor; Reference proteome.
FT CHAIN 1..78
FT /note="cAMP-dependent protein kinase inhibitor beta"
FT /id="PRO_0000154538"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..78
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 23
FT /note="Important for inhibition"
FT /evidence="ECO:0000250"
FT SITE 26
FT /note="Important for inhibition"
FT /evidence="ECO:0000250"
FT SITE 27
FT /note="Important for inhibition"
FT /evidence="ECO:0000250"
FT VAR_SEQ 1
FT /note="M -> MSHQDVAM (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046922"
SQ SEQUENCE 78 AA; 8468 MW; CA4AE267B9ECCD81 CRC64;
MRTDSSKMTD VESGVANFAS SARAGRRNAL PDIQSSAATD GTSDLPLKLE ALSVKEDAKE
KDEKTTQDQL EKPQNEEK
