ID   IPN2_LOTJA              Reviewed;         358 AA.
AC   E5L8F7;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   08-FEB-2011, sequence version 1.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Myb family transcription factor IPN2 {ECO:0000305};
DE   AltName: Full=Interacting protein of NSP2 {ECO:0000303|PubMed:24400899};
DE            Short=LjIPN2 {ECO:0000303|PubMed:24400899};
GN   Name=IPN2 {ECO:0000303|PubMed:24400899};
OS   Lotus japonicus (Lotus corniculatus var. japonicus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NSP2, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX   PubMed=24400899; DOI=10.1111/nph.12593;
RA   Kang H., Chu X., Wang C., Xiao A., Zhu H., Yuan S., Yang Z., Ke D.,
RA   Xiao S., Hong Z., Zhang Z.;
RT   "A MYB coiled-coil transcription factor interacts with NSP2 and is involved
RT   in nodulation in Lotus japonicus.";
RL   New Phytol. 201:837-849(2014).
CC   -!- FUNCTION: Transcriptional regulator required for Nod-factor-induced
CC       gene expression (PubMed:24400899). Transcription activator involved in
CC       the induction of NIN and ENOD40 genes, which are required for rhizobial
CC       infection and early nodule development (PubMed:24400899). Possesses
CC       strong transactivation activity in vitro (PubMed:24400899). Does not
CC       seem to contribute to the early steps of the arbuscular mycorrhizal
CC       fungus infection and colonization processes in roots (PubMed:24400899).
CC       {ECO:0000269|PubMed:24400899}.
CC   -!- SUBUNIT: Interacts with NSP2. {ECO:0000269|PubMed:24400899}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC       ECO:0000269|PubMed:24400899}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves, stems, nodules and roots.
CC       {ECO:0000269|PubMed:24400899}.
CC   -!- INDUCTION: Induced in roots after inoculation with Mesorhizobium loti.
CC       {ECO:0000269|PubMed:24400899}.
CC   -!- MISCELLANEOUS: Roots of plants silencing IPN2 exhibit decreased
CC       rhizobial infection and nodule organogenesis under nitrogen limitation
CC       when inoculated with Mesorhizobium loti. {ECO:0000269|PubMed:24400899}.
CC   -!- SIMILARITY: Belongs to the MYB-CC family. {ECO:0000305}.
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DR   EMBL; HQ343457; ADQ08683.1; -; mRNA.
DR   AlphaFoldDB; E5L8F7; -.
DR   SMR; E5L8F7; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0009877; P:nodulation; IEA:UniProtKB-KW.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR025756; Myb_CC_LHEQLE.
DR   InterPro; IPR017930; Myb_dom.
DR   InterPro; IPR006447; Myb_dom_plants.
DR   InterPro; IPR044848; PHR1-like.
DR   PANTHER; PTHR31499; PTHR31499; 1.
DR   Pfam; PF14379; Myb_CC_LHEQLE; 1.
DR   Pfam; PF00249; Myb_DNA-binding; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR   PROSITE; PS51294; HTH_MYB; 1.
PE   1: Evidence at protein level;
KW   Activator; Coiled coil; DNA-binding; Nodulation; Nucleus; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..358
FT                   /note="Myb family transcription factor IPN2"
FT                   /id="PRO_0000448274"
FT   DOMAIN          32..92
FT                   /note="HTH myb-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        63..88
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          310..358
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          127..171
FT                   /evidence="ECO:0000255"
FT   MOTIF           139..144
FT                   /note="LHEQLE"
FT                   /evidence="ECO:0000305"
FT   COMPBIAS        310..334
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        344..358
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   358 AA;  40180 MW;  05FF432E6BE707D0 CRC64;
     MERMFPPKKP STMNSHDRPM CVQGDSGLVL TTDPKPRLRW TVELHERFVD AVTQLGGPDK
     ATPKTIMRVM GVKGLTLYHL KSHLQKFRLG KQPHKEFNDH SIKDGMRASA LELQRNTASS
     SAMIGRNMNE MQIEVQRRLH EQLEVQKHLQ LRIEAQGKYM QSILEKAYQT LAGENMASAA
     TNLKGIGPQT IPDMGIMKEF GSPLGFSFQD LDLYGGGGGD QLELQQNMEK PPLDGFMPMN
     HENLCLGKKR PNPYSGNNGK SPLMWSDDLR LQDLGSCLQD DPFKGDHHHQ IQIAPPSLDR
     GTEMDPMSEI YDSKPEEKKF DASMKLERPS PRRAPLGERM SPMITTGTMA QGRSSPFG