IPO11_MOUSE
ID IPO11_MOUSE Reviewed; 975 AA.
AC Q8K2V6; Q8BU45; Q8K0B4;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Importin-11;
DE Short=Imp11;
DE AltName: Full=Ran-binding protein 11;
DE Short=RanBP11;
GN Name=Ipo11; Synonyms=Ranbp11;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Retina;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-842 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Ovary;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP FUNCTION, AND INTERACTION WITH RPL12.
RX PubMed=11809816; DOI=10.1128/mcb.22.4.1266-1275.2002;
RA Plafker S.M., Macara I.G.;
RT "Ribosomal protein L12 uses a distinct nuclear import pathway mediated by
RT importin 11.";
RL Mol. Cell. Biol. 22:1266-1275(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Functions in nuclear protein import as nuclear transport
CC receptor. Serves as receptor for nuclear localization signals (NLS) in
CC cargo substrates. Is thought to mediate docking of the
CC importin/substrate complex to the nuclear pore complex (NPC) through
CC binding to nucleoporin and the complex is subsequently translocated
CC through the pore by an energy requiring, Ran-dependent mechanism. At
CC the nucleoplasmic side of the NPC, Ran binds to the importin, the
CC importin/substrate complex dissociates and importin is re-exported from
CC the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The
CC directionality of nuclear import is thought to be conferred by an
CC asymmetric distribution of the GTP- and GDP-bound forms of Ran between
CC the cytoplasm and nucleus (By similarity). Mediates the nuclear import
CC of RPL12, and of UBE2E3 (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:11809816}.
CC -!- SUBUNIT: Interacts with UBE2E3 and RPL12.
CC {ECO:0000269|PubMed:11809816}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K2V6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K2V6-2; Sequence=VSP_010935;
CC -!- SIMILARITY: Belongs to the importin beta family. {ECO:0000305}.
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DR EMBL; BC029746; AAH29746.1; -; mRNA.
DR EMBL; BC031900; AAH31900.1; -; mRNA.
DR EMBL; AK087793; BAC40005.1; -; mRNA.
DR CCDS; CCDS36776.1; -. [Q8K2V6-1]
DR CCDS; CCDS88519.1; -. [Q8K2V6-2]
DR RefSeq; NP_083941.2; NM_029665.3. [Q8K2V6-1]
DR RefSeq; XP_006517849.1; XM_006517786.3.
DR AlphaFoldDB; Q8K2V6; -.
DR SMR; Q8K2V6; -.
DR BioGRID; 218189; 2.
DR IntAct; Q8K2V6; 2.
DR MINT; Q8K2V6; -.
DR STRING; 10090.ENSMUSP00000079667; -.
DR iPTMnet; Q8K2V6; -.
DR PhosphoSitePlus; Q8K2V6; -.
DR SwissPalm; Q8K2V6; -.
DR EPD; Q8K2V6; -.
DR MaxQB; Q8K2V6; -.
DR PaxDb; Q8K2V6; -.
DR PeptideAtlas; Q8K2V6; -.
DR PRIDE; Q8K2V6; -.
DR ProteomicsDB; 269078; -. [Q8K2V6-1]
DR ProteomicsDB; 269079; -. [Q8K2V6-2]
DR Antibodypedia; 23693; 218 antibodies from 26 providers.
DR DNASU; 76582; -.
DR Ensembl; ENSMUST00000080856; ENSMUSP00000079667; ENSMUSG00000042590. [Q8K2V6-1]
DR Ensembl; ENSMUST00000186033; ENSMUSP00000140046; ENSMUSG00000042590. [Q8K2V6-2]
DR GeneID; 76582; -.
DR KEGG; mmu:76582; -.
DR UCSC; uc007rtx.1; mouse. [Q8K2V6-1]
DR CTD; 51194; -.
DR MGI; MGI:2442377; Ipo11.
DR VEuPathDB; HostDB:ENSMUSG00000042590; -.
DR eggNOG; KOG1993; Eukaryota.
DR GeneTree; ENSGT00390000014071; -.
DR HOGENOM; CLU_003886_0_0_1; -.
DR InParanoid; Q8K2V6; -.
DR OMA; SFHYVFH; -.
DR OrthoDB; 228179at2759; -.
DR PhylomeDB; Q8K2V6; -.
DR TreeFam; TF324336; -.
DR BioGRID-ORCS; 76582; 26 hits in 74 CRISPR screens.
DR ChiTaRS; Ipo11; mouse.
DR PRO; PR:Q8K2V6; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q8K2V6; protein.
DR Bgee; ENSMUSG00000042590; Expressed in floor plate of midbrain and 257 other tissues.
DR ExpressionAtlas; Q8K2V6; baseline and differential.
DR Genevisible; Q8K2V6; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IDA:MGI.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0006606; P:protein import into nucleus; IBA:GO_Central.
DR GO; GO:0006610; P:ribosomal protein import into nucleus; IDA:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR001494; Importin-beta_N.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Repeat; Transport.
FT CHAIN 1..975
FT /note="Importin-11"
FT /id="PRO_0000120757"
FT DOMAIN 28..100
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REPEAT 123..160
FT /note="HEAT 1"
FT REPEAT 283..317
FT /note="HEAT 2"
FT REPEAT 318..356
FT /note="HEAT 3"
FT REPEAT 422..459
FT /note="HEAT 4"
FT REPEAT 473..509
FT /note="HEAT 5"
FT REPEAT 511..548
FT /note="HEAT 6"
FT REPEAT 555..593
FT /note="HEAT 7"
FT REPEAT 600..636
FT /note="HEAT 8"
FT REPEAT 640..677
FT /note="HEAT 9"
FT REPEAT 683..720
FT /note="HEAT 10"
FT REPEAT 731..773
FT /note="HEAT 11"
FT REPEAT 819..849
FT /note="HEAT 12"
FT REPEAT 850..887
FT /note="HEAT 13"
FT REPEAT 957..974
FT /note="HEAT 14"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q9UI26"
FT MOD_RES 343
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UI26"
FT VAR_SEQ 698
FT /note="L -> LPSSCLANEL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_010935"
FT CONFLICT 349
FT /note="A -> V (in Ref. 2; BAC40005)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 975 AA; 112416 MW; 3DEA79E8028C483D CRC64;
MDLNSASSVV LQVLTQATSQ DTAVLKPAEE QLKQWETQPG FYSVLLNIFT NHTLDINVRW
LAVLYFKHGI DRYWRRVAPH ALSEEEKSTL RAGLITNFNE PINQIATQIA VLIAKVARLD
CPRQWPELIP TLVESVKVQD DLRQHRALLT FYHVTKTLAS KRLAADRKLF YDLASGIYNF
ACSLWNHHTD TFLQHVSSGN EAAVLSSLER TLLSLKVLRK LTVNGFVEPH KNMEVMGFLH
GIFERLKQFL ECSRSIGTDN VCRDRLEKTI ILFTKVLLDF LDQHPISFTP LIQRSLEFSV
SYVFTEVGEG VTFERFIVQC MNLIKMIVKN YAYKPSKNFE DSSPETLEAH KIKMAFFTYP
TLTEICRRLV SHYFLLTEEE LTMWEEDPEG FTVEETGGDS WKYSLRPCTE VLFIDIFHEY
NQTLTPVLLE MMQTLEGPTN VEDMNALLIK DAVYNAVGLA AFELFDSVDF DQWFKTQLLP
ELQVSHNRYK PLRRRVIWLI GQWISVKFKS DLRPMLYEAI CNLLQDQDLV VRIETATTLK
LTVDDFEFRT DQFLPYLETM FTLLFQLLQQ VTECDTKMHV LHVLSCVIER VNVQIRPYVG
CLVQYLPLLW KQSEEHNMLR CAILTTLIHL VQGLGADSKN LYPFLLPVIQ LSTDVSQPPH
VYLLEDGLEL WLVTLENSPC VTPELLRIFQ NMSPLLELSS ENLRTCFKII NGYIFLSSTE
FLQTYAAGLC QSFYELLPEI TTEGQVQVLK VVENALKVNP VLGPQMFQRI LPCVFRGVIE
GERYPVVMSI YLAVMGRVLL QNTSFFSSLL NEMGHEFNQE MDQLLGNVIE MWVDRMDNIT
QPERKKLSAL ALLSLLPSDN SVIQDKFCGI INISVEALHD VMTEDPETRT YKDCMLMSQH
EEPKVTEDEE PPTEQDKRKK MLALKDPVHT VSLQQFIYEK LKAQQEILGE QGFQSLMETV
DTEIVTQLQE FLQGF
