IPO13_MOUSE
ID IPO13_MOUSE Reviewed; 963 AA.
AC Q8K0C1; Q6ZQ60;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Importin-13;
DE Short=Imp13;
GN Name=Ipo13; Synonyms=Kiaa0724;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Embryo;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [2]
RP SEQUENCE REVISION.
RA Okazaki N., Kikuno R., Nagase T., Ohara O., Koga H.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Heart;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Functions in nuclear protein import as nuclear transport
CC receptor. Serves as receptor for nuclear localization signals (NLS) in
CC cargo substrates. Is thought to mediate docking of the
CC importin/substrate complex to the nuclear pore complex (NPC) through
CC binding to nucleoporin and the complex is subsequently translocated
CC through the pore by an energy requiring, Ran-dependent mechanism. At
CC the nucleoplasmic side of the NPC, Ran binds to the importin, the
CC importin/substrate complex dissociates and importin is re-exported from
CC the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The
CC directionality of nuclear import is thought to be conferred by an
CC asymmetric distribution of the GTP- and GDP-bound forms of Ran between
CC the cytoplasm and nucleus (By similarity). Mediates the nuclear import
CC of UBC9, the RBM8A/MAGOH complex, PAX6 and probably other members of
CC the paired homeobox family. Also mediates nuclear export of eIF-1A, and
CC the cytoplasmic release of eIF-1A is triggered by the loading of import
CC substrates onto IPO13 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with UBC9, RAN, RBM8A, eIF-1A and PAX6.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8K0C1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8K0C1-2; Sequence=VSP_010936, VSP_010937;
CC -!- SIMILARITY: Belongs to the importin beta family. {ECO:0000305}.
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DR EMBL; AK129200; BAC98010.2; -; Transcribed_RNA.
DR EMBL; BC031823; AAH31823.1; -; mRNA.
DR EMBL; AK052257; BAC34899.1; -; mRNA.
DR CCDS; CCDS18542.1; -. [Q8K0C1-1]
DR RefSeq; NP_666264.1; NM_146152.3. [Q8K0C1-1]
DR AlphaFoldDB; Q8K0C1; -.
DR SMR; Q8K0C1; -.
DR BioGRID; 230998; 2.
DR STRING; 10090.ENSMUSP00000035989; -.
DR iPTMnet; Q8K0C1; -.
DR PhosphoSitePlus; Q8K0C1; -.
DR EPD; Q8K0C1; -.
DR MaxQB; Q8K0C1; -.
DR PaxDb; Q8K0C1; -.
DR PeptideAtlas; Q8K0C1; -.
DR PRIDE; Q8K0C1; -.
DR ProteomicsDB; 269080; -. [Q8K0C1-1]
DR ProteomicsDB; 269081; -. [Q8K0C1-2]
DR Antibodypedia; 32405; 119 antibodies from 20 providers.
DR DNASU; 230673; -.
DR Ensembl; ENSMUST00000036156; ENSMUSP00000035989; ENSMUSG00000033365. [Q8K0C1-1]
DR GeneID; 230673; -.
DR KEGG; mmu:230673; -.
DR UCSC; uc008ujg.1; mouse. [Q8K0C1-1]
DR CTD; 9670; -.
DR MGI; MGI:2385205; Ipo13.
DR VEuPathDB; HostDB:ENSMUSG00000033365; -.
DR eggNOG; KOG2022; Eukaryota.
DR GeneTree; ENSGT00530000063347; -.
DR HOGENOM; CLU_005996_3_0_1; -.
DR InParanoid; Q8K0C1; -.
DR OMA; DTFMYCY; -.
DR OrthoDB; 1032285at2759; -.
DR PhylomeDB; Q8K0C1; -.
DR TreeFam; TF314539; -.
DR BioGRID-ORCS; 230673; 27 hits in 73 CRISPR screens.
DR PRO; PR:Q8K0C1; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8K0C1; protein.
DR Bgee; ENSMUSG00000033365; Expressed in hindlimb stylopod muscle and 228 other tissues.
DR Genevisible; Q8K0C1; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0035259; F:nuclear glucocorticoid receptor binding; ISO:MGI.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0006606; P:protein import into nucleus; ISO:MGI.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR013598; Exportin-1/Importin-b-like.
DR InterPro; IPR001494; Importin-beta_N.
DR InterPro; IPR040709; Importin_rep_1.
DR InterPro; IPR040944; Importin_rep_2.
DR InterPro; IPR040520; Importin_rep_3.
DR Pfam; PF03810; IBN_N; 1.
DR Pfam; PF18773; Importin_rep; 1.
DR Pfam; PF18786; Importin_rep_2; 2.
DR Pfam; PF18806; Importin_rep_3; 1.
DR Pfam; PF08389; Xpo1; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Protein transport;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..963
FT /note="Importin-13"
FT /id="PRO_0000120759"
FT REPEAT 24..54
FT /note="HEAT 1"
FT DOMAIN 45..111
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REPEAT 56..88
FT /note="HEAT 2"
FT REPEAT 95..135
FT /note="HEAT 3"
FT REPEAT 142..179
FT /note="HEAT 4"
FT REPEAT 194..231
FT /note="HEAT 5"
FT REPEAT 236..268
FT /note="HEAT 6"
FT REPEAT 276..325
FT /note="HEAT 7"
FT REPEAT 330..372
FT /note="HEAT 8"
FT REPEAT 375..438
FT /note="HEAT 9"
FT REPEAT 440..476
FT /note="HEAT 10"
FT REPEAT 487..522
FT /note="HEAT 11"
FT REPEAT 524..558
FT /note="HEAT 12"
FT REPEAT 562..600
FT /note="HEAT 13"
FT REPEAT 603..648
FT /note="HEAT 14"
FT REPEAT 676..716
FT /note="HEAT 15"
FT REPEAT 720..754
FT /note="HEAT 16"
FT REPEAT 761..803
FT /note="HEAT 17"
FT REPEAT 815..845
FT /note="HEAT 18"
FT REPEAT 860..893
FT /note="HEAT 19"
FT REPEAT 897..931
FT /note="HEAT 20"
FT VAR_SEQ 369..375
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_010936"
FT VAR_SEQ 872..886
FT /note="AIGGQASRSLMDCFA -> VSWSKWLGVGRPSLLRRLALCCG (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:14621295"
FT /id="VSP_010937"
SQ SEQUENCE 963 AA; 108229 MW; 3ABBCE15668B6BCB CRC64;
MERREEQLGA AGAGAAPALD FTVENVEKAL HQLYYDPNID NKNLAQKWLM QAQVSPQAWH
FSWQLLQPDK VPEIQYFGAS ALHIKISRYW SDIPTDQYES LKAQLFTQIT RFASGSKIVL
TRLCVALASL ALSMMPDAWP CAVADMVRLF QAEDSPVDSQ GRCLALLELL TVLPEEFQTS
RLPQYRKGLV RTSLAVECGT VFPLLEQLLQ QPSSPSCVRQ KVLKCFSSWV QLEVPLQDCE
ALIQAAFAAL QDSELFDSSV EAIVNAISQP DAQRYVNTLL KLIPLVLGLQ EQLRQAVQNG
DMETSHGICR IAVALGENHS RALLDQVEHW QSFLALVNMI MFCTGIPGHY PVNETTSSLT
LTFWYTLQDD ILSFEAEKQA VYQQVYRPVY FQLVDVLLHK AQFPSDEEYG FWSSDEKEQF
RIYRVDISDT LMYVYEMLGA ELLSNLYDKL GRLLTSSEEP YSWQHTEALL YGFQSIAETI
DVNYSDVVPG LIGLIPRISI SNVQLADTVM FTIGALSEWL ADHPVMINSV LPLVLHALGN
PELSVSSVST LKKICRECKY DLPPYAANIV AVSQDVLMKQ IHKTSQCMWL MQALGFLLSA
LQVEEILKNL HSLISPYIQQ LEKLAEEIPN PSNKLAIVHI LGLLSNLFTT LDVSHHEDDH
EGPELRKLPV PQGPNPVVVV LQQVFQLIQK VLSKWLNDAQ VVEAVCAIFE KSVKTLLDDF
APMVPQLCEM LGRMYSTVPQ ASALDLTRQL VHIFAHEPAH FPPIEALFLL VTSVTLSLFQ
QGPRDHPDIV DSFMQLLAQA LKRKPDLFLC ERLDVKAVFQ CAVLALKFPE APTVKASCGF
FTELLPRCGE IESVGKVVQE DGRMLLIAVL EAIGGQASRS LMDCFADILF ALNKHCFSLL
SMWIKEALQP PGFPSARLSP EQKDTFSQQI LRERVNKRRV KEMVKEFTLL CRGLHGTDYT
ADY
