IPO9_DROME
ID IPO9_DROME Reviewed; 1018 AA.
AC Q9VGP4;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Importin-9 {ECO:0000303|PubMed:33632744};
DE AltName: Full=Ran-binding protein 9 {ECO:0000303|Ref.1};
GN Name=Ipo9 {ECO:0000303|PubMed:33632744, ECO:0000312|FlyBase:FBgn0037894};
GN Synonyms=Ranbp9 {ECO:0000303|Ref.1, ECO:0000312|FlyBase:FBgn0037894};
GN ORFNames=CG5252 {ECO:0000312|FlyBase:FBgn0037894};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hartmann E., Goerlich D.;
RT "Drosophila melanogaster RanBP9.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=33632744; DOI=10.1242/jcs.258391;
RA Palacios V., Kimble G.C., Tootle T.L., Buszczak M.;
RT "Importin-9 regulates chromosome segregation and packaging in Drosophila
RT germ cells.";
RL J. Cell Sci. 134:0-0(2021).
CC -!- FUNCTION: Nuclear transport receptor that mediates nuclear import of
CC proteins (PubMed:33632744). Serves as receptor for nuclear localization
CC signals (NLS) in cargo substrates (By similarity). Is thought to
CC mediate docking of the importin/substrate complex to the nuclear pore
CC complex (NPC) through binding to nucleoporin and the complex is
CC subsequently translocated through the pore by an energy requiring, Ran-
CC dependent mechanism (By similarity). Mediates the import of pre-
CC assembled proteasomes into the nucleus during the late stages of sperm
CC development (PubMed:33632744). {ECO:0000250|UniProtKB:Q96P70,
CC ECO:0000269|PubMed:33632744}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:33632744}. Nucleus
CC {ECO:0000269|PubMed:33632744}.
CC -!- DISRUPTION PHENOTYPE: Male and female sterility caused by disruption of
CC chromosome segregation and condensation during meiosis
CC (PubMed:33632744). Impaired nuclear localization of proteasome
CC components (PubMed:33632744). Males form abnormally structured sperm
CC and fail to properly exchange histones for protamines
CC (PubMed:33632744). {ECO:0000269|PubMed:33632744}.
CC -!- SIMILARITY: Belongs to the importin beta family. {ECO:0000305}.
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DR EMBL; AF245516; AAF73427.1; -; mRNA.
DR EMBL; AY128454; AAM75047.1; -; mRNA.
DR EMBL; AE014297; AHN57283.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF54632.1; -; Genomic_DNA.
DR RefSeq; NP_001287284.1; NM_001300355.1.
DR RefSeq; NP_650068.1; NM_141811.2.
DR AlphaFoldDB; Q9VGP4; -.
DR SMR; Q9VGP4; -.
DR IntAct; Q9VGP4; 1.
DR STRING; 7227.FBpp0081862; -.
DR PaxDb; Q9VGP4; -.
DR PRIDE; Q9VGP4; -.
DR EnsemblMetazoa; FBtr0082386; FBpp0081862; FBgn0037894.
DR EnsemblMetazoa; FBtr0346638; FBpp0312218; FBgn0037894.
DR GeneID; 41367; -.
DR KEGG; dme:Dmel_CG5252; -.
DR UCSC; CG5252-RA; d. melanogaster.
DR CTD; 10048; -.
DR FlyBase; FBgn0037894; Ipo9.
DR VEuPathDB; VectorBase:FBgn0037894; -.
DR eggNOG; KOG2274; Eukaryota.
DR GeneTree; ENSGT00390000008224; -.
DR HOGENOM; CLU_008920_1_0_1; -.
DR InParanoid; Q9VGP4; -.
DR OMA; FINCIVT; -.
DR OrthoDB; 546100at2759; -.
DR PhylomeDB; Q9VGP4; -.
DR BioGRID-ORCS; 41367; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Ranbp9; fly.
DR GenomeRNAi; 41367; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037894; Expressed in ovary and 25 other tissues.
DR ExpressionAtlas; Q9VGP4; baseline and differential.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005654; C:nucleoplasm; IDA:FlyBase.
DR GO; GO:0061608; F:nuclear import signal receptor activity; ISS:FlyBase.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0031144; P:proteasome localization; IMP:UniProtKB.
DR GO; GO:0006606; P:protein import into nucleus; IMP:FlyBase.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR001494; Importin-beta_N.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Nucleus; Protein transport; Reference proteome; Transport.
FT CHAIN 1..1018
FT /note="Importin-9"
FT /id="PRO_0000455442"
FT DOMAIN 35..114
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT REGION 921..950
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1018 AA; 114407 MW; A7E353A722477F1E CRC64;
MSLQFQNDCG DSVKQAIIEE LQNLLSSDTG VLQQTEKRIK QLEYTEGYGV YLSEIIMNQA
HELPLRQIAI VMLTRYVENH WTDDDDVKRK ANGCMASEQA KRTIRNILPN GLYDPNSKIR
SSVAHTISTI AATDYPHCWA ELFDIIVKCL GGNEDSIHGA MQVLQDFSYD VEQIKELGPV
VIPEVYRIFD SEQNYSIKTR VSAIRILKPL FASIAALITN KEEQSTMMSS ILTNFMDKLM
HYLSMNSGAV SSFLLRTEII KVFTHLVNEM PKYINPFMDR VLPIVWQLLT QIAETYVKVS
VNQTETSPLA SGDSEEDDEQ TNFQSLIIQI LEFINCILTC NKLRGSIKNV LADLIYITIV
YIQLSEEQLE DWHDDPEKFV DDEDDGGVEL TVRMCGRDVL LAINDEFGAN AIQPLQEALG
RHFSVADAEK AANNPNWWKI QEACMDAVHA FRDVILAGDS TFDLLNYLTI VRNLLVHQES
PPLVGRALWT LSIYSKSDLY NPQMLTEILD VTLCSLSPEK SHILRISAVR TLNGFLQANE
TIDGEKRTLL VSKLPGFLDG IMALVPGSKA AVLALLMEAL TFMVKFDAEF AFASQAKITP
LAIAVFLKYT EDPYVLETVQ DLIKALCQRK ECLGPLQEKF IPTIVSILGL TGAASTEKQD
IALDVLNTIV RYTEPPLNNS LLETAFPAII NCVLHTDDHA VMVAGGECLR SFINVSPEQI
CSYKNGEGIN CIMQVVATVL LNPMNSEMTA AGQIGRLVIT IITKMGSMLG QNVDMLLKAV
ISKMQNLECL KVIMNLVLIF AHLFLTQMDA VLNFLSTVPG PNGEPAMQFV LTNWLSRQNS
FFGNYERKVT TMALCKLFEY GVATQDNRLT TITFKELVDD PTDTRRRTRS VAATTQKWVT
IPALVKIFKV LISEYQHFQE GKSDEPLTDS EEDGDDEDAP GNPDKPRYIS DLFESDEDNA
EDEQLLQELL KETNYQGDIA DNLQKFLTTF TQNEHFPTFY EHLTEGERLI LLSKVQQK
