IPOU_DROME
ID IPOU_DROME Reviewed; 396 AA.
AC P24350; O77214; Q26465; Q59E44; Q59E45; Q8IR36; Q9VXW1;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 3.
DT 03-AUG-2022, entry version 190.
DE RecName: Full=Inhibitory POU protein;
DE Short=I-POU;
DE AltName: Full=Abnormal chemosensory jump 6 protein;
GN Name=acj6; Synonyms=Ipou; ORFNames=CG9151;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), AND TISSUE SPECIFICITY.
RX PubMed=1673230; DOI=10.1038/350577a0;
RA Treacy M.N., He X., Rosenfeld M.G.;
RT "I-POU: a POU-domain protein that inhibits neuron-specific gene
RT activation.";
RL Nature 350:577-584(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S;
RX PubMed=10069339; DOI=10.1016/s0896-6273(00)81094-6;
RA Clyne P.J., Certel S.J., de Bruyne M., Zaslavsky L., Johnson W.A.,
RA Carlson J.R.;
RT "The odor specificities of a subset of olfactory receptor neurons are
RT governed by Acj6, a POU-domain transcription factor.";
RL Neuron 22:339-347(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 318-327 (ISOFORMS B/C/D), AND
RP FUNCTION.
RX PubMed=1346754; DOI=10.1016/0092-8674(92)90186-g;
RA Treacy M.N., Neilson L.I., Turner E.E., He X., Rosenfeld M.G.;
RT "Twin of I-POU: a two amino acid difference in the I-POU homeodomain
RT distinguishes an activator from an inhibitor of transcription.";
RL Cell 68:491-505(1992).
RN [6]
RP DNA-BINDING.
RX PubMed=8986770; DOI=10.1073/pnas.93.26.15097;
RA Turner E.E.;
RT "Similar DNA recognition properties of alternatively spliced Drosophila POU
RT factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:15097-15101(1996).
CC -!- FUNCTION: Modulates gene transcription; simultaneously generates both a
CC specific activator and an inhibitor of gene transcription, capable of
CC modulating two distinct regulatory programs during neural development.
CC Has a role in olfactory behavior. {ECO:0000269|PubMed:10069339,
CC ECO:0000269|PubMed:1346754}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=A; Synonyms=TI-POU, Twin-of-I-POU;
CC IsoId=P24350-2; Sequence=Displayed;
CC Name=C; Synonyms=I-POU;
CC IsoId=P24350-1; Sequence=VSP_018342, VSP_018343, VSP_002342;
CC Name=B;
CC IsoId=P24350-3; Sequence=VSP_018342, VSP_002342;
CC Name=D;
CC IsoId=P24350-4; Sequence=VSP_002342;
CC -!- TISSUE SPECIFICITY: Coexpressed with vvl in overlapping subsets of
CC neurons in the embryonic central nervous system. Expressed in olfactory
CC neurons. {ECO:0000269|PubMed:10069339, ECO:0000269|PubMed:1673230}.
CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-4
CC subfamily. {ECO:0000305}.
CC -!- CAUTION: Was originally thought that the I-POU homeobox is unable to
CC bind DNA because it lacks two N-terminal basic residues.
CC {ECO:0000305|PubMed:1673230}.
CC -!- CAUTION: Was originally thought to interact with vvl.
CC {ECO:0000305|PubMed:1673230}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA41342.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X58436; CAA41342.1; ALT_FRAME; mRNA.
DR EMBL; AF086816; AAC35369.1; -; mRNA.
DR EMBL; AE014298; AAF48447.2; -; Genomic_DNA.
DR EMBL; AE014298; AAN09663.1; -; Genomic_DNA.
DR EMBL; AE014298; AAX52497.1; -; Genomic_DNA.
DR EMBL; AE014298; AAX52498.1; -; Genomic_DNA.
DR EMBL; AH003854; AAB21441.1; -; Genomic_DNA.
DR PIR; A42089; A42089.
DR PIR; S14795; S14795.
DR RefSeq; NP_001014743.1; NM_001014743.3. [P24350-4]
DR RefSeq; NP_001014744.1; NM_001014744.2. [P24350-1]
DR RefSeq; NP_524876.1; NM_080137.3. [P24350-2]
DR RefSeq; NP_727845.1; NM_167438.2. [P24350-3]
DR AlphaFoldDB; P24350; -.
DR SMR; P24350; -.
DR BioGRID; 70572; 5.
DR STRING; 7227.FBpp0073832; -.
DR PaxDb; P24350; -.
DR DNASU; 47080; -.
DR EnsemblMetazoa; FBtr0074015; FBpp0073832; FBgn0000028. [P24350-2]
DR EnsemblMetazoa; FBtr0100326; FBpp0099732; FBgn0000028. [P24350-1]
DR EnsemblMetazoa; FBtr0100328; FBpp0099734; FBgn0000028. [P24350-4]
DR EnsemblMetazoa; FBtr0290063; FBpp0288502; FBgn0000028. [P24350-3]
DR GeneID; 47080; -.
DR KEGG; dme:Dmel_CG9151; -.
DR CTD; 47080; -.
DR FlyBase; FBgn0000028; acj6.
DR VEuPathDB; VectorBase:FBgn0000028; -.
DR eggNOG; KOG1168; Eukaryota.
DR GeneTree; ENSGT00940000169264; -.
DR InParanoid; P24350; -.
DR PhylomeDB; P24350; -.
DR Reactome; R-DME-6804759; Regulation of TP53 Activity through Association with Co-factors.
DR BioGRID-ORCS; 47080; 0 hits in 3 CRISPR screens.
DR ChiTaRS; acj6; fly.
DR GenomeRNAi; 47080; -.
DR PRO; PR:P24350; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000028; Expressed in brain and 22 other tissues.
DR ExpressionAtlas; P24350; baseline and differential.
DR Genevisible; P24350; DM.
DR GO; GO:0005634; C:nucleus; ISS:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0003714; F:transcription corepressor activity; IGI:FlyBase.
DR GO; GO:0007411; P:axon guidance; IMP:FlyBase.
DR GO; GO:0007636; P:chemosensory jump behavior; IMP:FlyBase.
DR GO; GO:0070983; P:dendrite guidance; IMP:FlyBase.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IDA:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0008039; P:synaptic target recognition; IMP:FlyBase.
DR CDD; cd00086; homeodomain; 1.
DR CDD; cd00093; HTH_XRE; 1.
DR Gene3D; 1.10.260.40; -; 1.
DR InterPro; IPR001387; Cro/C1-type_HTH.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR InterPro; IPR013847; POU.
DR InterPro; IPR000327; POU_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00157; Pou; 1.
DR PRINTS; PR00028; POUDOMAIN.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00352; POU; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF47413; SSF47413; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00035; POU_1; 1.
DR PROSITE; PS00465; POU_2; 1.
DR PROSITE; PS51179; POU_3; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Homeobox; Nucleus; Olfaction;
KW Reference proteome; Repressor; Sensory transduction; Transcription;
KW Transcription regulation.
FT CHAIN 1..396
FT /note="Inhibitory POU protein"
FT /id="PRO_0000100775"
FT DOMAIN 222..299
FT /note="POU-specific"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT DNA_BIND 320..377
FT /note="Homeobox; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 302..328
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 86..95
FT /note="POU-IV box"
FT VAR_SEQ 54..72
FT /note="Missing (in isoform B and isoform C)"
FT /evidence="ECO:0000303|PubMed:1673230"
FT /id="VSP_018342"
FT VAR_SEQ 132..138
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|PubMed:1673230"
FT /id="VSP_018343"
FT VAR_SEQ 323..324
FT /note="Missing (in isoform B, isoform C and isoform D)"
FT /evidence="ECO:0000303|PubMed:1673230"
FT /id="VSP_002342"
FT CONFLICT 166
FT /note="L -> V (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 216
FT /note="A -> P (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="L -> V (in Ref. 1; CAA41342)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 43704 MW; AEA80A7EF633B30D CRC64;
MTMSMYSTTD KMKMSAPSCF PGRYSPSYRS SEQMRRCMPN PSIHISSSCD SLESRLLEDA
SLLCNSWSAR QNGDIFAGIN DGILSRAEAL AAVDIQKHQA QHVHSQMPSQ IKHDVMYHHH
SMSGPPQRPL QENPFSRQMH HSMDQLDMLD PTGSMTTLAP ISESPLTPTH QHLHGSYHSM
NHMMSHHHPG TLSGHTGGHH GHSAVHHPVI TAAVAAAGLH PDTDTDPREL EAFAERFKQR
RIKLGVTQAD VGKALANLKL PGVGALSQST ICRFESLTLS HNNMIALKPI LQAWLEEAEA
QAKNKRRDPD APSVLPAGEK KRKRTSIAAP EKRSLEAYFA VQPRPSGEKI AAIAEKLDLK
KNVVRVWFCN QRQKQKRIVS SVTPSMTGHG SAGFGY
