IPP2B_HUMAN
ID IPP2B_HUMAN Reviewed; 205 AA.
AC Q6NXS1; G1AUC5;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 28-MAR-2018, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein phosphatase inhibitor 2 family member B {ECO:0000305};
DE AltName: Full=PPP1R2 family member B;
DE AltName: Full=Protein phosphatase 1, regulatory subunit 2 pseudogene 3;
DE AltName: Full=Protein phosphatase inhibitor 2-like protein 3;
GN Name=PPP1R2B {ECO:0000312|HGNC:HGNC:16318}; Synonyms=PPP1R2P3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=21382349; DOI=10.1016/j.bcp.2011.02.018;
RA Fardilha M., Korrodi-Gregorio L., Vintem A.P., Domingues S.C.T.S.,
RA Rebelo S., Morrice N., Cohen P.T.W., da Cruz e Silva O.A.B.,
RA da Cruz e Silva E.F.;
RT "Identification of the human testis protein phosphatase 1 interactome.";
RL Biochem. Pharmacol. 82:1403-1415(2011).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY,
RP PHOSPHORYLATION AT SER-121; SER-122 AND SER-127, AND INTERACTION WITH
RP PPP1CC.
RX PubMed=23506001; DOI=10.1186/1471-2121-14-15;
RA Korrodi-Gregorio L., Ferreira M., Vintem A.P., Wu W., Muller T., Marcus K.,
RA Vijayaraghavan S., Brautigan D.L., da Cruz E Silva O.A., Fardilha M.,
RA da Cruz E Silva E.F.;
RT "Identification and characterization of two distinct PPP1R2 isoforms in
RT human spermatozoa.";
RL BMC Cell Biol. 14:15-15(2013).
CC -!- FUNCTION: Inhibitor of protein-phosphatase 1.
CC {ECO:0000269|PubMed:23506001}.
CC -!- SUBUNIT: Interacts with PPP1CC. {ECO:0000269|PubMed:23506001}.
CC -!- INTERACTION:
CC Q6NXS1; Q7Z2G1: H2BW1; NbExp=3; IntAct=EBI-10251630, EBI-18200422;
CC Q6NXS1; P62136: PPP1CA; NbExp=3; IntAct=EBI-10251630, EBI-357253;
CC Q6NXS1; P62140: PPP1CB; NbExp=8; IntAct=EBI-10251630, EBI-352350;
CC Q6NXS1; P36873: PPP1CC; NbExp=5; IntAct=EBI-10251630, EBI-356283;
CC -!- TISSUE SPECIFICITY: Only detected in spermatozoa, both heads and tails.
CC {ECO:0000269|PubMed:23506001}.
CC -!- SIMILARITY: Belongs to the protein phosphatase inhibitor 2 family.
CC {ECO:0000305}.
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DR EMBL; JF438008; AEB77711.1; -; mRNA.
DR EMBL; AC011414; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC066922; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS87340.1; -.
DR AlphaFoldDB; Q6NXS1; -.
DR IntAct; Q6NXS1; 4.
DR GlyGen; Q6NXS1; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q6NXS1; -.
DR MetOSite; Q6NXS1; -.
DR PhosphoSitePlus; Q6NXS1; -.
DR BioMuta; PPP1R2P3; -.
DR DMDM; 74736994; -.
DR EPD; Q6NXS1; -.
DR jPOST; Q6NXS1; -.
DR MassIVE; Q6NXS1; -.
DR PeptideAtlas; Q6NXS1; -.
DR PRIDE; Q6NXS1; -.
DR ProteomicsDB; 66768; -.
DR TopDownProteomics; Q6NXS1; -.
DR Antibodypedia; 79322; 15 antibodies from 6 providers.
DR Ensembl; ENST00000522232.3; ENSP00000490297.1; ENSG00000231989.5.
DR MANE-Select; ENST00000522232.3; ENSP00000490297.1; NM_206858.3; NP_996740.2.
DR GeneCards; PPP1R2B; -.
DR HGNC; HGNC:16318; PPP1R2B.
DR HPA; ENSG00000231989; Tissue enriched (testis).
DR neXtProt; NX_Q6NXS1; -.
DR OpenTargets; ENSG00000231989; -.
DR VEuPathDB; HostDB:ENSG00000231989; -.
DR GeneTree; ENSGT00390000004757; -.
DR InParanoid; Q6NXS1; -.
DR OMA; YHGIMGD; -.
DR PathwayCommons; Q6NXS1; -.
DR SignaLink; Q6NXS1; -.
DR Pharos; Q6NXS1; Tdark.
DR PRO; PR:Q6NXS1; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q6NXS1; protein.
DR Bgee; ENSG00000231989; Expressed in left testis and 8 other tissues.
DR GO; GO:0004864; F:protein phosphatase inhibitor activity; IBA:GO_Central.
DR GO; GO:0005977; P:glycogen metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0043666; P:regulation of phosphoprotein phosphatase activity; IBA:GO_Central.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR InterPro; IPR007062; PPI-2.
DR PANTHER; PTHR12398; PTHR12398; 1.
DR Pfam; PF04979; IPP-2; 1.
PE 1: Evidence at protein level;
KW Acetylation; Carbohydrate metabolism; Glycogen metabolism; Phosphoprotein;
KW Protein phosphatase inhibitor; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P11845"
FT CHAIN 2..205
FT /note="Protein phosphatase inhibitor 2 family member B"
FT /id="PRO_0000286138"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 12..17
FT /note="Required for binding PPP1CC"
FT /evidence="ECO:0000250"
FT REGION 43..55
FT /note="Required for binding PPP1CC"
FT /evidence="ECO:0000250"
FT REGION 111..142
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..150
FT /note="Required for binding PPP1CC catalytic center,
FT displacing metal ions and inhibition of PPP1CC catalytic
FT activity"
FT /evidence="ECO:0000250"
FT REGION 163..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..30
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 166..180
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..205
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:P11845"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P41236"
FT MOD_RES 89
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P41236"
FT MOD_RES 92
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P41236"
FT MOD_RES 121
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:23506001"
FT MOD_RES 122
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:23506001"
FT MOD_RES 127
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:23506001"
FT MOD_RES 130
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P50411"
FT CONFLICT 98
FT /note="D -> G (in Ref. 3; BC066922)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 205 AA; 23106 MW; 69AF42846313A728 CRC64;
MAASTASHRP IKGILKNKTS TTSSMVASAE QPRRSVDEEL SKKSQKWDEI NILATYHPAD
KGYGLMKIDE PSPPYHSMMG DDEDACRDTE TTEAMAPDIL AKKLAAAEGL EPKYRIQEQE
SSGEEDSDLS PEEREKKRQF EMRRKLHYNE GLNIKLARQL ISKDLHDDDE DEEMLETADG
ESMNTEESNQ GSTPSDQQQN KLRSS
