IPT6_ARATH
ID IPT6_ARATH Reviewed; 342 AA.
AC Q9C6L1;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Adenylate isopentenyltransferase 6, chloroplastic;
DE Short=AtIPT6;
DE EC=2.5.1.112;
DE AltName: Full=Adenylate dimethylallyltransferase 6;
DE AltName: Full=Cytokinin synthase 6;
DE Flags: Precursor;
GN Name=IPT6; OrderedLocusNames=At1g25410; ORFNames=F2J7.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=11313355; DOI=10.1074/jbc.m102130200;
RA Takei K., Sakakibara H., Sugiyama T.;
RT "Identification of genes encoding adenylate isopentenyltransferase, a
RT cytokinin biosynthesis enzyme, in Arabidopsis thaliana.";
RL J. Biol. Chem. 276:26405-26410(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND GENE FAMILY.
RC STRAIN=cv. Wassilewskija;
RX PubMed=11479373; DOI=10.1093/pcp/pce112;
RA Kakimoto T.;
RT "Identification of plant cytokinin biosynthetic enzymes as dimethylallyl
RT diphosphate:ATP/ADP isopentenyltransferases.";
RL Plant Cell Physiol. 42:677-685(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=14675438; DOI=10.1046/j.1365-313x.2003.01945.x;
RA Miyawaki K., Matsumoto-Kitano M., Kakimoto T.;
RT "Expression of cytokinin biosynthetic isopentenyltransferase genes in
RT Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and
RT nitrate.";
RL Plant J. 37:128-138(2004).
CC -!- FUNCTION: Involved in cytokinin biosynthesis. Catalyzes the transfer of
CC an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and
CC ADP. {ECO:0000269|PubMed:11313355}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ADP + dimethylallyl diphosphate = diphosphate + N(6)-
CC (dimethylallyl)adenosine 5'-diphosphate; Xref=Rhea:RHEA:36327,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:73533,
CC ChEBI:CHEBI:456216; EC=2.5.1.112;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + dimethylallyl diphosphate = diphosphate + N(6)-
CC (dimethylallyl)adenosine 5'-triphosphate; Xref=Rhea:RHEA:36331,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57623,
CC ChEBI:CHEBI:73532; EC=2.5.1.112;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in siliques, at the mRNA level.
CC {ECO:0000269|PubMed:14675438}.
CC -!- MISCELLANEOUS: Due to a nucleotide deletion that would cause a
CC frameshift, IPT6 seems to be a pseudogene in cv. Wassilewskija.
CC -!- SIMILARITY: Belongs to the IPP transferase family. {ECO:0000305}.
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DR EMBL; AB062612; BAB59045.1; -; mRNA.
DR EMBL; AC079281; AAG50809.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30619.1; -; Genomic_DNA.
DR PIR; B86384; B86384.
DR RefSeq; NP_173912.1; NM_102352.1.
DR AlphaFoldDB; Q9C6L1; -.
DR SMR; Q9C6L1; -.
DR STRING; 3702.AT1G25410.1; -.
DR PaxDb; Q9C6L1; -.
DR PRIDE; Q9C6L1; -.
DR ProteomicsDB; 247040; -.
DR EnsemblPlants; AT1G25410.1; AT1G25410.1; AT1G25410.
DR GeneID; 839127; -.
DR Gramene; AT1G25410.1; AT1G25410.1; AT1G25410.
DR KEGG; ath:AT1G25410; -.
DR Araport; AT1G25410; -.
DR TAIR; locus:2031205; AT1G25410.
DR eggNOG; KOG1384; Eukaryota.
DR HOGENOM; CLU_032616_4_1_1; -.
DR InParanoid; Q9C6L1; -.
DR OMA; SRELRYN; -.
DR OrthoDB; 1003231at2759; -.
DR PhylomeDB; Q9C6L1; -.
DR BRENDA; 2.5.1.112; 399.
DR PRO; PR:Q9C6L1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6L1; baseline and differential.
DR Genevisible; Q9C6L1; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0052623; F:ADP dimethylallyltransferase activity; ISS:TAIR.
DR GO; GO:0009824; F:AMP dimethylallyltransferase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0052622; F:ATP dimethylallyltransferase activity; ISS:TAIR.
DR GO; GO:0052381; F:tRNA dimethylallyltransferase activity; IBA:GO_Central.
DR GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006400; P:tRNA modification; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR039657; Dimethylallyltransferase.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11088; PTHR11088; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chloroplast; Cytokinin biosynthesis; Nucleotide-binding;
KW Plastid; Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..33
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 34..342
FT /note="Adenylate isopentenyltransferase 6, chloroplastic"
FT /id="PRO_0000391074"
FT BINDING 52..59
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 342 AA; 38363 MW; 65292BEC0FD626D9 CRC64;
MQQLMTLLSP PLSHSSLLPT VTTKFGSPRL VTTCMGHAGR KNIKDKVVLI TGTTGTGKSR
LSVDLATRFF PAEIINSDKM QIYKGFEIVT NLIPLHEQGG VPHHLLGQFH PQDGELTPAE
FRSLATLSIS KLISSKKLPI VVGGSNSFNH ALLAERFDPD IDPFSPGSSL STICSDLRYK
CCILWVDVLE PVLFQHLCNR VDQMIESGLV EQLAELYDPV VDSGRRLGVR KTIGVEEFDR
YFRVYPKEMD KGIWDLARKA AYEETVKGMK ERTCRLVKKQ KEKIMKLIRG GWEIKRLDAT
AAIMAELNQS TAKGEGKNGR EIWEKHIVDE SVEIVKKFLL EV
