ID   IPTZ_AGRT7              Reviewed;         243 AA.
AC   P06524;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
DE   AltName: Full=Trans-zeatin producing protein;
GN   Name=tzs;
OS   Agrobacterium tumefaciens (strain T37).
OG   Plasmid pTiT37.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176300;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987868; DOI=10.1093/nar/13.8.2773;
RA   Akiyoshi D.E., Regier D.A., Jen G.C., Gordon M.P.;
RT   "Cloning and nucleotide sequence of the tzs gene from Agrobacterium
RT   tumefaciens strain T37.";
RL   Nucleic Acids Res. 13:2773-2788(1985).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
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DR   EMBL; X02423; CAA26275.1; -; Genomic_DNA.
DR   PIR; A23000; JJAG3Z.
DR   RefSeq; NP_053379.1; NC_002147.1.
DR   AlphaFoldDB; P06524; -.
DR   SMR; P06524; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; Cytokinin biosynthesis; Plasmid; Transferase.
FT   CHAIN           1..243
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216442"
SQ   SEQUENCE   243 AA;  27577 MW;  13D9D5F60693DD6E CRC64;
     MILHLIYGPT CSGKTDMAIQ IAQETGWPVV ALDRVQCCPQ IATGSGRPLE SELQSTRRIY
     LDSRPLTEGI LDAESAHRRL IFEVDWRKSE DGLILEGGSI SLLNCMAKSP FWRSGFQWHV
     KRLRLGDSDA FLTRAKQRVA EMFAIREDRP SLLEELAELW NYPAARPILE DIDGYRCAIR
     FARKHDLAIS QLPNIDAGRH VELIEAIANE YLEHALSQER DFPQWPEDGA GQPVCPVTLT
     RIR