ID   IPT_AGRT4               Reviewed;         240 AA.
AC   P03869;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
GN   Name=izt; Synonyms=tmr;
OS   Agrobacterium tumefaciens (strain Ach5).
OG   Plasmid pTiAch5.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176298;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6312414; DOI=10.1093/nar/11.18.6211;
RA   Heidekamp F., Dirkse W.G., Hille J., van Ormondt H.;
RT   "Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmid-
RT   encoded tmr gene.";
RL   Nucleic Acids Res. 11:6211-6223(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6327292; DOI=10.1002/j.1460-2075.1984.tb01894.x;
RA   Gielen J., de Beuckeleer M., Seurinck J., Deboeck F., de Greve H.,
RA   Lemmers M., van Montagu M., Schell J.;
RT   "The complete nucleotide sequence of the TL-DNA of the Agrobacterium
RT   tumefaciens plasmid pTiAch5.";
RL   EMBO J. 3:835-846(1984).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; X00010; CAA24911.1; -; Genomic_DNA.
DR   EMBL; X00493; CAA25170.1; -; Genomic_DNA.
DR   PIR; A00589; JJAG5T.
DR   RefSeq; NP_059677.1; NC_002377.1.
DR   AlphaFoldDB; P03869; -.
DR   SMR; P03869; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; Cytokinin biosynthesis; Plasmid; Transferase.
FT   CHAIN           1..240
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216431"
SQ   SEQUENCE   240 AA;  27004 MW;  AD9D0AD553A5106A CRC64;
     MDLHLIFGPT CTGKTTTAIA LAQQTGLPVL SLDRVQCCPQ LSTGSGRPTV EELKGTTRLY
     LDDRPLVEGI IAAKQAHHRL IEEVYNHEAN GGLILEGGST SLLNCMARNS YWSADFRWHI
     IRHKLPDQET FMKAAKARVK QMLHPAAGHS IIQELVYLWN EPRLRPILKE IDGYRYAMLF
     ASQNQITADM LLQLDANMEG KLINGIAQEY FIHARQQEQK FPQVNAAAFD GFEGHPFGMY