ID   IPT_AGRT7               Reviewed;         240 AA.
AC   P0A3L6; P04380;
DT   20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-1987, sequence version 1.
DT   25-MAY-2022, entry version 53.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
GN   Name=izt; Synonyms=ipt, tmr;
OS   Agrobacterium tumefaciens (strain T37).
OG   Plasmid pTiT37.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176300;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6330678; DOI=10.1093/nar/12.11.4665;
RA   Goldberg S.B., Flick J.S., Rogers S.G.;
RT   "Nucleotide sequence of the tmr locus of Agrobacterium tumefaciens pTi T37
RT   T-DNA.";
RL   Nucleic Acids Res. 12:4665-4677(1984).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=16593495; DOI=10.1073/pnas.81.15.4776;
RA   Barry G.F., Rogers S.G., Fraley R.T., Brand L.;
RT   "Identification of a cloned cytokinin biosynthetic gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:4776-4780(1984).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000269|PubMed:16593495}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC         Evidence={ECO:0000269|PubMed:16593495};
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; X00639; CAA25268.1; -; Genomic_DNA.
DR   PIR; A00588; JJAG3T.
DR   RefSeq; NP_053424.1; NC_002147.1.
DR   AlphaFoldDB; P0A3L6; -.
DR   SMR; P0A3L6; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Crown gall tumor; Cytokinin biosynthesis; Plasmid; Transferase.
FT   CHAIN           1..240
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216433"
SQ   SEQUENCE   240 AA;  27025 MW;  350859D21B97D817 CRC64;
     MDLRLIFGPT CTGKTSTAVA LAQQTGLPVL SLDRVQCCPQ LSTGSGRPTV EELKGTSRLY
     LDDRPLVKGI IAAKQAHERL MGEVYNYEAH GGLILEGGSI SLLKCMAQSS YWSADFRWHI
     IRHELADEET FMNVAKARVK QMLRPAAGLS IIQELVDLWK EPRLRPILKE IDGYRYAMLF
     ASQNQITSDM LLQLDADMED KLIHGIAQEY LIHARRQEQK FPRVNAAAYD GFEGHPFGMY