ID   IPT_AGRVI               Reviewed;         240 AA.
AC   P25018;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
GN   Name=ipt;
OS   Agrobacterium vitis (Rhizobium vitis).
OG   Plasmid pTiTM4.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium.
OX   NCBI_TaxID=373;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TM4;
RX   PubMed=2546041; DOI=10.1007/bf00334387;
RA   Bonnard G., Tinland B., Paulus F., Szegedi E., Otten L.;
RT   "Nucleotide sequence, evolutionary origin and biological role of a
RT   rearranged cytokinin gene isolated from a wide host range biotype III
RT   Agrobacterium strain.";
RL   Mol. Gen. Genet. 216:428-438(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CG474;
RA   Otten L., de Ruffray P.;
RL   Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; X56185; CAA39647.1; -; Genomic_DNA.
DR   EMBL; X17428; CAA35471.1; -; Genomic_DNA.
DR   EMBL; U83987; AAB41875.1; -; Genomic_DNA.
DR   RefSeq; WP_032488312.1; NZ_WPHX01000055.1.
DR   AlphaFoldDB; P25018; -.
DR   SMR; P25018; -.
DR   GeneID; 60685090; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; Cytokinin biosynthesis; Plasmid; Transferase.
FT   CHAIN           1..240
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216435"
SQ   SEQUENCE   240 AA;  26814 MW;  0E433C9BA7E6EC3D CRC64;
     MDLRLIFGPT CTGKTSTAIA LAQQTGLPVL SLDRVQCCPQ LSTGSGRPTV EELKGTTRLY
     LDDRPLVKGI IAAEQAHERL IAEVYNYEAH GGLILEGGSI SLLKCMAQSG YWSADFRWHI
     IRHKLADEET FMKAAKARVK QMLCPAIGPS LIQELVYLWN EPRLRPILKE IDGYRYAMLF
     ASQNRITPDM LLQLDADMEG KLIHGIAQEY LIHARRQEHE FPPVSAAAFE GFEGPPFGAY