ID   IPT_DICDI               Reviewed;         283 AA.
AC   Q86K86; Q550C3;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 103.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Isopentenyl transferase;
GN   Name=iptA; ORFNames=DDB_G0277215;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=12097910; DOI=10.1038/nature00847;
RA   Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA   Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA   Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA   Noegel A.A.;
RT   "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL   Nature 418:79-85(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   CATALYTIC ACTIVITY.
RX   PubMed=11710395; DOI=10.1038/271545a0;
RA   Taya Y., Tanaka Y., Nishimura S.;
RT   "5'-AMP is a direct precursor of cytokinin in Dictyostelium discoideum.";
RL   Nature 271:545-547(1978).
RN   [4]
RP   FUNCTION.
RX   PubMed=18216168; DOI=10.1242/dev.018051;
RA   Anjard C., Loomis W.F.;
RT   "Cytokinins induce sporulation in Dictyostelium.";
RL   Development 135:819-827(2008).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones like isopentenyl adenine or
CC       discadenine which controle spore formation and viability.
CC       {ECO:0000269|PubMed:18216168}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC         Evidence={ECO:0000269|PubMed:11710395};
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000019; EAL68793.1; -; Genomic_DNA.
DR   RefSeq; XP_642693.1; XM_637601.1.
DR   AlphaFoldDB; Q86K86; -.
DR   SMR; Q86K86; -.
DR   STRING; 44689.DDB0233672; -.
DR   PaxDb; Q86K86; -.
DR   EnsemblProtists; EAL68793; EAL68793; DDB_G0277215.
DR   GeneID; 8620883; -.
DR   KEGG; ddi:DDB_G0277215; -.
DR   dictyBase; DDB_G0277215; iptA.
DR   eggNOG; ENOG502RHQV; Eukaryota.
DR   HOGENOM; CLU_984925_0_0_1; -.
DR   InParanoid; Q86K86; -.
DR   OMA; VINYIEY; -.
DR   PRO; PR:Q86K86; -.
DR   Proteomes; UP000002195; Chromosome 2.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IMP:dictyBase.
DR   GO; GO:0052381; F:tRNA dimethylallyltransferase activity; IBA:GO_Central.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IDA:dictyBase.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IDA:dictyBase.
DR   GO; GO:0006400; P:tRNA modification; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   Cytokinin biosynthesis; Reference proteome; Sporulation; Transferase.
FT   CHAIN           1..283
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000365733"
SQ   SEQUENCE   283 AA;  32393 MW;  FED51CA2112ADC59 CRC64;
     MKILLIIGST GVGKTDLSIN YSKKYNAPVV VLDRIQCFPE LSITSGRPDE SEYFGSKRIY
     LTDLLVEPGN ENIKKTFYVN KLINILNEIK NNYDTQNLPN EKGYGCIFEG GSISLLKELL
     TKINKLPYKI TCVIYIRPSD SIDNHKLYKA KIFKRVSQML FPTEEGNDSQ ILEVKRILNK
     GKTVNAQGEI NLEYYEKIKQ VLISLVGLVG IEDVIHFLDK QYDQKNITSK LDPNYLNEIQ
     SQLIETITLA HYNYALSQIE LIDSLIKQLP KSIEYCLKNI EIN