ID   IPT_PSESS               Reviewed;         234 AA.
AC   P06619;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Adenylate dimethylallyltransferase;
DE            EC=2.5.1.27;
DE   AltName: Full=Dimethylallyl transferase;
DE   AltName: Full=Isopentenyl transferase;
DE   AltName: Full=Trans-zeatin producing protein;
GN   Name=ptz;
OS   Pseudomonas savastanoi (Pseudomonas syringae pv. savastanoi).
OG   Plasmid pCK1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=29438;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1006;
RX   PubMed=3515320; DOI=10.1093/nar/14.6.2555;
RA   Powell G.K., Morris R.O.;
RT   "Nucleotide sequence and expression of a Pseudomonas savastanoi cytokinin
RT   biosynthetic gene: homology with Agrobacterium tumefaciens tmr and tzs
RT   loci.";
RL   Nucleic Acids Res. 14:2555-2565(1986).
CC   -!- FUNCTION: Transfers dimethylallyl groups to AMP as part of the
CC       biosynthesis of cytokinin phytohormones. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=AMP + dimethylallyl diphosphate = diphosphate + N(6)-
CC         (dimethylallyl)adenosine 5'-phosphate; Xref=Rhea:RHEA:15285,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57526, ChEBI:CHEBI:57623,
CC         ChEBI:CHEBI:456215; EC=2.5.1.27;
CC   -!- SIMILARITY: Belongs to the isopentenyl transferase family.
CC       {ECO:0000305}.
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DR   EMBL; X03679; CAA27315.1; -; Genomic_DNA.
DR   PIR; A24937; A24937.
DR   RefSeq; WP_031598371.1; NZ_NIAX01000031.1.
DR   AlphaFoldDB; P06619; -.
DR   SMR; P06619; -.
DR   GO; GO:0009824; F:AMP dimethylallyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009691; P:cytokinin biosynthetic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR039657; Dimethylallyltransferase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR002648; Tzs.
DR   PANTHER; PTHR11088; PTHR11088; 1.
DR   PANTHER; PTHR11088:SF28; PTHR11088:SF28; 1.
DR   PIRSF; PIRSF000507; IPT; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Cytokinin biosynthesis; Plasmid; Transferase.
FT   CHAIN           1..234
FT                   /note="Adenylate dimethylallyltransferase"
FT                   /id="PRO_0000216439"
SQ   SEQUENCE   234 AA;  26811 MW;  1380875F5390BD9A CRC64;
     MKIYLIWGAT CTGKTEHSIK LSKSTGWPVI VLDRVQCCFD IATGSGRPHP EELQSTRRIY
     LDNRRISEGV ISAEEANDRL KLEVNKHIDS GGVILEGGSI SLLKLISKDP YWCDRFIWSQ
     HRMRLQDTDV FMDKAKARVR RMLVGSTETT GLLDELVAAQ SDLNAKLAIQ DIDGYRYIMN
     YAQARRLSIT QLLNVMTGDM KEELINGIAL EYYEHAKWQE RDFPAEWLAE RSTR